Development of a sensitive and specific enzyme-linked immunosorbent assay based on recombinant antigens for rapid detection of antibodies against Mycoplasma agalactiae in sheep

Clinical and Vaccine Immunology

Volumn 14, Issue 4, 2007, Pages 420-425

  Fusco, Marisa ^a   Corona, Lucia ^a   Onni, Toniangelo ^a   Marras, Elisabetta ^b   Longheu, Carla ^a   Idini, Graziano ^a   Tola, Sebastiana ^a  

^a ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA SARDEGNA   (Italy)

^b Edificio 3 Loc Piscinamanna ^*  (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; PROTEIN P55; PROTEIN P80; RECOMBINANT ANTIGEN;

AGALACTIA; ANIMAL EXPERIMENT; ANIMAL MODEL; ANTIBODY DETECTION; ANTIBODY RESPONSE; ARTICLE; BACTERIAL INFECTION; BACTERIAL STRAIN; BLOOD SAMPLING; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPERIMENTAL INFECTION; FEMALE; INSTRUMENTATION; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; MYCOPLASMA AGALACTIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RECEIVER OPERATING CHARACTERISTIC; SENSITIVITY AND SPECIFICITY; SERODIAGNOSIS; SHEEP; STATISTICAL SIGNIFICANCE; WESTERN BLOTTING;

ANIMALS; ANTIBODIES, BACTERIAL; ANTIGENS, BACTERIAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; MYCOPLASMA AGALACTIAE; MYCOPLASMA INFECTIONS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY; SHEEP;

EID: 34248345117     PISSN: 15566811     EISSN: None     Source Type: Journal    
DOI: 10.1128/CVI.00439-06     Document Type: Article

References (22)

1. Bergonier, D., X. Berthelot, and F. Poumarat. 1997. Contagious agalactia of small ruminants: current knowledge concerning epidemiology, diagnosis and control. Rev. Sci. Tech. Off. Int. Epizoot. 16:848-873.
2. Clyde, W. A. 1983. Growth inhibition tests, p. 405-410. In S. Razin and J. Tully (ed.), Methods in mycoplasmology, vol. 1. Mycoplasma characterization. Academic Press, New York, NY.
3. Fleury, B., D. Bergonier, X. Berthelot, Y. Schlatter, J. Frey, and E. M. Vilei. 2001. Characterization and analysis of a stable serotype-associated membrane protein (P30) of Mycoplasma agalactiae. J. Clin. Microbiol. 39:2814-2822.
4. Fleury, B., D. Bergonier, X. Berthelot, E. Peterhans, J. Frey, and E. M. Vilei. 2002. Characterization of P40, a cytoadhesin of Mycoplasma agalactiae. Infect. Immun. 70:5612-5621.
5. Flitman-Tene, R., S. Levisohn, I. Lysnyansky, E. Rapoport, and D. Yogev. 2000. A chromosomal region of Mycoplasma agalactiae containing vsp-related genes undergoes in vivo rearrangement in naturally infected animals. FEMS Microbiol. Lett. 191:205-212.
6. Glew, M. D., L. Papazisi, F. Poumarat, D. Bergonier, R. Rosengarten, and C. Citti. 2000. Characterization of a multigene family undergoing high-frequency DNA rearrangements and coding for abundant variable surface proteins in Mycoplasma agalactiae. Infect. Immun. 68:4539-4548.
7. Hanley, J. A., and B. J. McNeil. 1982. The meaning and use of the area under receiver operating characteristic (ROC) curve. Radiology 143:29-36.
8. Hanley, J. A., and B. J. McNeil. 1983. A method of comparing the areas under receiver operating characteristic curves derived from the same cases. Radiology 148:839-843.
9. Kittelberger, R., J. S. O'Keefe, R. Meynell, M. Sewell, S. Rosati, M. Lambert, P. Dufour, and M. Pepin. 2006. Comparison of four diagnostic tests for the identification of serum antibodies in small ruminants infected with Mycoplasma agalactiae. N. Z. Vet. J. 54:10-15.
10. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
11. Levisohn, S., I. Davidson, M. R. Caro Vergara, and E. Rapoport. 1991. Use of an ELISA for differential diagnosis of Mycoplasma agalactiae and M. mycoides subspecies mycoides (LC) in naturally infected goat herds. Res. Vet. Sci. 51:66-71.
12. Pepin, M., P. Dufour, M. Lambert, M. Aubert, A. Valognes, T. Rotis, A. Van de Wiele, and D. Bergonier. 2003. Comparison of three enzyme-linked immunosorbent assays for serologic diagnosis of contagious agalactia in sheep. J. Vet. Diagn. Investig. 15:281-285.
13. Rodwell, A. W., and R. F. Whitcomb. 1983. Methods for direct and indirect measurement of mycoplasma growth, p. 185-196. In S. Razin and J. Tully (ed.), Methods in mycoplasmology, vol. 1. Mycoplasma characterization. Academic Press, New York, NY.
14. Rosati, S., S. Pozzi, P. Robino, B. Montinaro, A. Conti, M. Fadda, and M. Pittau. 1999. P48 major surface antigen of Mycoplasma agalactiae is homologous to a malp product of Mycoplasma fermentans and belongs to a selected family of bacterial lipoprotein. Infect. Immun. 67:6213-6216.
15. Rosati, S., P. Robino, M. Fadda, S. Pozzi, A. Mannelli, and M. Pittau. 2000. Expression and antigenic characterization of recombinant Mycoplasma agalactiae P48 major surface protein. Vet. Microbiol. 71:201-210.
16. Santona, A., F. Carta, P. Fraghì, and F. Turrini. 2002. Mapping antigenic sites of an immunodominant surface lipoprotein of Mycoplasma agalactiae, AvgC, with the use of synthetic peptides. Infect. Immun. 70:171-176.
17. Solsona, M., M. Lambert, and F. Poumarat. 1996. Genomic, protein homogeneity and antigenic variability of Mycoplasma agalactiae. Vet. Microbiol. 50:45-58.
18. Tola, S., G. Idini, D. Manunta, G. Galleri, A. Angioi, A. M. Rocchigiani, and G. Leori. 1996. Rapid and specific detection of Mycoplasma agalactiae by polymerase chain reaction. Vet. Microbiol. 51:77-84.
19. Tola, S., G. Idini, D. Manunta, I. Casciano, A. M. Rocchigiani, A. Angioi, and G. Leori. 1996. Comparison of Mycoplasma agalactiae isolates by pulsed field gel electrophoresis, SDS-PAGE and immunoblotting. FEMS Microbiol. Lett. 143:259-265.
20. Tola, S., A. Angioi, A. M. Rocchigiani, G. Idini, D. Manunta, G. Galleri, and G. Leori. 1997. Detection of Mycoplasma agalactiae in sheep milk samples by polymerase chain reaction. Vet. Microbiol. 54:17-22.
21. Tola, S., D. Manunta, M. Cocco, F. Turrini, A. M. Rocchigiani, G. Idini, A. Angioi, and G. Leori. 1997. Characterization of membrane surface proteins of Mycoplasma agalactiae during natural infection. FEMS Microbiol. Lett. 154:355-362.
22. Tola, S., S. Crobeddu, G. Chessa, S. Uzzau, G. Idini, B. Ibba, and S. Rocca. 2001. Sequence, cloning, expression and characterisation of the 81-kDa surface membrane protein (P80) of Mycoplasma agalactiae. FEMS Microbiol. Lett. 202:45-50.