Structural and mechanistic studies reveal the functional role of bicovalent flavinylation in berberine bridge enzyme

Journal of Biological Chemistry

Volumn 284, Issue 30, 2009, Pages 19993-20001

  Winkler, Andreas ^a   Motz, Kerstin ^a   Riedl, Sabrina ^a,c   Puhl, Martin ^a   Macheroux, Peter ^a   Gruber, Karl ^b  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF GRAZ   (Austria)

^c ERICH SCHMID INSTITUTE OF MATERIALS SCIENCE   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

CATALYZED REACTIONS; COFACTOR; COVALENT LINKAGE; CYSTEINE RESIDUES; DUAL MODES; EXPRESSION LEVELS; FLAVIN COFACTOR; FLAVINYLATION; ISOLATION AND PURIFICATION; MECHANISTIC STUDIES; METHYL GROUP; MUTANT PROTEINS; OVER-EXPRESSION; PROTEIN DISULFIDE ISOMERASE; PROTEIN VARIANTS; REDOX POTENTIALS; STRUCTURAL CHARACTERIZATION; SUBSTRATE BINDING; WILD-TYPE ENZYMES; X RAY CRYSTAL STRUCTURES;

CHAINS; ENZYMES; HYDROCARBONS; SECONDARY BATTERIES;

PROTEIN FOLDING;

BERBERINE BRIDGE ENZYME; BERBERINE DERIVATIVE; MUTANT PROTEIN; PROTEIN C166A; PROTEIN H104A; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL REACTION; CONTROLLED STUDY; COVALENT BOND; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME METABOLISM; ENZYME STRUCTURE; ENZYME SUBSTRATE; MUTANT; OXIDATION REDUCTION POTENTIAL; PRIORITY JOURNAL; PROTEIN EXPRESSION; WILD TYPE;

CRYSTALLOGRAPHY, X-RAY; ESCHSCHOLZIA; FLAVIN-ADENINE DINUCLEOTIDE; GENE EXPRESSION; KINETICS; MODELS, MOLECULAR; MUTANT PROTEINS; OXIDATION-REDUCTION; OXIDOREDUCTASES, N-DEMETHYLATING; PROTEIN BINDING; PROTEIN CONFORMATION;

EID: 67749096156     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.015727     Document Type: Article

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