Analysis of PCR-RAPD DNA and antibiotic susceptibility profiles of antrum and corpus isolates of Helicobacter pylori from Malaysian patients

Malaysian Journal of Pathology

Volumn 31, Issue 1, 2009, Pages 29-34

  Norazah, Ahmad ^a   Wan Rasinah, Z ^a   Zaili, Z ^a   Aminuddin, A ^b   Ramelah, M ^c  

^a INSTITUTE FOR MEDICAL RESEARCH   (Malaysia)

^b UNIVERSITI TEKNOLOGI MARA   (Malaysia)

^c UNIVERSITI KEBANGSAAN MALAYSIA   (Malaysia)

Author keywords

Helicobacter pylori; Malaysian patients; Metronidazole resistance; PCR RAPD

Indexed keywords

AMOXICILLIN; CIPROFLOXACIN; CLARITHROMYCIN; LEVOFLOXACIN; METRONIDAZOLE; TETRACYCLINE;

ADULT; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATE; CLINICAL ARTICLE; CLUSTER ANALYSIS; EPSILOMETER TEST; FEMALE; GENETIC HETEROGENEITY; HELICOBACTER INFECTION; HELICOBACTER PYLORI; HUMAN; HUMAN TISSUE; MALAYSIA; MALE; METHODOLOGY; MINIMUM INHIBITORY CONCENTRATION; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA; STOMACH ANTRUM;

ADULT; ANTI-BACTERIAL AGENTS; DNA, BACTERIAL; DRUG RESISTANCE, BACTERIAL; FEMALE; HELICOBACTER INFECTIONS; HELICOBACTER PYLORI; HUMANS; MALE; METRONIDAZOLE; MICROBIAL SENSITIVITY TESTS; MIDDLE AGED; PHYLOGENY; POLYMERASE CHAIN REACTION; POLYMORPHISM, GENETIC; PYLORIC ANTRUM; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE;

EID: 67749094904     PISSN: 01268635     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Gottke MU, Fallone CA, Barkun AN, et al. Genetic variability determinants of Helicobacter pylori: influence of clinical background and geographic origin isolates. J Infect Dis 2000; 181: 1674-1681
2. Caroll IM, Ahmed N, Beesley SM, et al. Fine-struture molecular typing of Irish Helicobacter isolates and their genetic relatedness to strains from four different continents. J Clin Microbiol 2003; 41: 5755-5759
3. Berg DE, Gilman RH, Lelwala-Guruge J, et al. Helicobacter pylori populations in individual Peruvian patients. Clin Infect Dis 1997; 25: 996-1002.
4. Caroll IM, Ahmed N, Beesley SM., et al. Microevolution between paired antral and paired antrum and corpus Helicobacter pylori isolates recovered from individual patients. J Med Microbiol 2004; 53: 669-677
5. Han FC, Ng HC, Ho B. Stability of randomly amplified polymorphic DNA fingerprinting in genotyping clinical isolates of Helicobacter pylori. World J Gastroenterol 2003; 9: 2021-2024 (Pubitemid 37168435)
6. Kim JJ, Kim JG, Kwon DH. Mixed infection of antibiotic susceptible and resistant Helicobacter pylori isolates in a single patient and underestimation of antimicrobial susceptibility testing. Helicobacter 2003; 8: 202-206
7. Raymond J, Nguyen B, Bergeret M, Dupont C, Kalach N. Heterogenous susceptibility to metronidazole and clarithromycin of Helicobacter pylori isolates from a single biopsy in adults is confirmed in children. Int J Antimicrob Agents 2005; 26: 272-278
8. Weel JFL, van der Hulst RWM, Gerrits Y, Tytgat GNJ, van der Ende A, Dankert J. Heterogeneity in susceptibility to metronidazole among Helicobacter pylori isolates from patients with gastritis or peptic ulcer disease. J Clin Microbiol 1996; 34: 2158-2162
9. Kim JM, Kim JS, Kim N, Jung HC, Song IS. Distribution of fluoroquinolone MICs in Helicobacter pylori strains from Korean patients. J Antimicrob Chemother 2005; 56: 965-967
10. Andrews JM. BSAC standardized disc susceptibility testing method (version 7). J Antimicrob Chemother 2008; 62: 256-278
11. Burucoa C, Lhomme V, Fauchere JL. Performance criteria of DNA fingerprinting methods for typing of Helicobacter pylori isolates: experimental results and meta-analysis. J Clin Microbiol 1999; 37: 4071-4080
12. Wong BC, Wang WH, Berg DE, et al. High prevalence of mixed infections by Helicobacter pylori in Hong Kong: metronidazole sensitivity and overall genotype. Aliment Pharmacol Ther 2001; 15: 493-503.
13. Thoreson AC, Hosseini N, Svennerholm AM, Bolin I. Different Helicobacter pylori strains colonize the antral and duodenal mucosa of duodenal ulcer patients. Helicobacter. 2000; 5: 69-78. (Pubitemid 34948011)
14. Marshall BJ, McCallum RW, Gluerrant RL. Helicobacter pylori in peptic ulceration and gastritis. Blackwell Scientific Publications; 1991.
15. Miehlke S, Thomas R, Guiterrez O, Graham DY, Go MF. DNA fingerprinting of single colonies of Helicobacter pylori from gastric cancer patients suggests infection with a single predominant strain. J Clin Microbiol 1999; 37: 245-247
16. Jorgensen M, Daskalopoulos G, Waburton V, Mitchel HM, Hazell SL. Multiple strain colonization and metronidazole resistance in Helicobacter pyloriinfected patients: identification from sequential and multiple biopsy specimens. J Infect Dis 1996; 174: 631-635
17. Yakoob J, Fan XG, Hu GL, et al. Polycolonization of Helicobacter pylori among Chinese subjects. Clin Microbiol Inf Dis 2001; 7: 187-192
18. Kuo CH, Poon SK, Su YC, Su R, Chang CS, Wang WC. Heterogeneous Helicobacter pylori isolates from H. pylori-infected couples in Taiwan. J Infect Dis 1999; 180: 2064-2068
19. Kim JW, Kim JG, Chae SL, Cha YJ, Park SM. High prevalence of multiple strain colonization of Helicobacter pylori in Korean patients: DNA diversity among clinical isolates from the gastric corpus, antrum and duodenum. Korean J Intern Med. 2004; 19: 1-9.
20. Van der Wouden EJ, de Jong A, Thijs JC, Kleibeuker JH, van Zwer AA. Subpopulations of Helicobacter pylori are responsible for discrepancies in the outcome of nitroimidazole susceptibility testing. Antimicrob Agents Chemother 1999; 43: 1484-1486