Fluorescence correlation spectroscopy of phosphatidylinositol-specific phospholipase C monitors the interplay of substrate and activator lipid binding

Biochemistry

Volumn 48, Issue 29, 2009, Pages 6835-6845

  Pu, Mingming ^a   Roberts, Mary F ^a   Gershenson, Anne ^b  

^a BOSTON COLLEGE   (United States)

^b BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVITY MEASUREMENTS; ANIONIC LIPIDS; BACILLUS THURINGIENSIS; CATALYTIC ACTIVITY; DIRECT ANALYSIS; ENZYMATIC ACTIVITIES; FLUORESCENCE CORRELATION SPECTROSCOPY; LIPID BINDING; MEMBRANE AFFINITY; MEMBRANE BINDING; MEMBRANE SURFACE; MOLE FRACTION; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLINOSITOL; PHOSPHOLIPASE C; SINGLE MUTATION; WILD TYPES;

BACTERIOLOGY; CATALYST ACTIVITY; ELECTROCHEMICAL SENSORS; ENZYMES; FLUORESCENCE SPECTROSCOPY; LIPIDS; PLANTS (BOTANY); SUBSTRATES;

BINDING SITES;

PHOSPHATIDYLINOSITOL; PHOSPHOLIPASE C;

ARTICLE; BACILLUS THURINGIENSIS; BINDING AFFINITY; DIMERIZATION; FLUORESCENCE CORRELATION SPECTROSCOPY; MEMBRANE VESICLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN INTERACTION;

BACILLUS THURINGIENSIS; BIOCATALYSIS; LIPID METABOLISM; MODELS, MOLECULAR; PHOSPHATIDYLINOSITOLS; SPECTROMETRY, FLUORESCENCE; SUBSTRATE SPECIFICITY; TYPE C PHOSPHOLIPASES;

BACILLUS THURINGIENSIS;

EID: 67651230535     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi900633p     Document Type: Article

References (27)

1. Johnson, J. E., and Cornell, R. B. (1999) Amphitropic proteins: regulation by reversible membrane interactions. Mol. Membr. Biol. 16, 217-235.
2. Gelb, M. H., Cho, W., and Wilton, D. C. (1999) Interfacial binding of secreted phospholipases A2: more than electrostatics and a major role for tryptophan. Curr. Opin. Struct. Biol. 9, 428-432.
3. Daugherty, S., and Low, M. G. (1993) Cloning, expression, and mutagenesis of phosphatidylinositol-specific phospholipase C from Staphylococcus aureus: a potential staphylococcal virulence factor. Infect. Immun. 61, 5078-5089.
4. Mengaud, J., Braun-Breton, C., and Cossart, P. (1991) Identification of phosphatidylinositol-specific phospholipase C activity in Listeria monocytogenes: a novel type of virulence factor? Mol. Microbiol. 5, 367-372.
5. Hirose, S., Knez, J. J., Edward Medof, M., Patrick, J. C., and Janice, E. B. (1995) Mammalian glycosylphosphatidylinositol-anchored proteins and intracellular precursors. Methods Enzymol. 250, 582-614.
6. Zhou, C., Qian, X., and Roberts, M. F. (1997) Allosteric activation of phosphatidylinositol-specific phospholipase C: specific phospholipid binding anchors the enzyme to the interface. Biochemistry 36, 10089-10097.
7. Zhou, C., and Roberts, M. F. (1998) Nonessential activation and competitive inhibition of bacterial phosphatidylinositol-specific phospholipase C by short-chain phospholipids and analogues. Biochemistry 37, 16430-16439.
8. Zhou, C., Wu, Y., and Roberts, M. F. (1997) Activation of phosphatidylinositol-specific phospholipase C toward inositol 1,2-(cyclic)-phosphate. Biochemistry 36, 347-355.
9. Feng, J., Bradley, W. D., and Roberts, M. F. (2003) Optimizing the interfacial binding and activity of a bacterial phosphatidylinositol-specific phospholipase C. J. Biol. Chem. 278, 24651-24657.
10. Feng, J., Wehbi, H., and Roberts, M. F. (2002) Role of tryptophan residues in interfacial binding of phosphatidylinositol-specific phospholipase C. J. Biol. Chem. 277, 19867-19875.
11. Heinz, D. W., Ryan, M., Bullock, T. L., and Griffith, O. H. (1995) Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol. EMBO J. 14, 3855-3863.
12. Shao, C., Shi, X., Wehbi, H., Zambonelli, C., Head, J. F., Seaton, B. A., and Roberts, M. F. (2007) Dimer structure of an interfacially impaired phosphatidylinositol-specific phospholipase C. J. Biol. Chem. 282, 9228-9235.
13. Guo, S., Zhang, X., Seaton, B. A., and Roberts, M. F. (2008) Role of helix B residues in interfacial activation of a bacterial phosphatidylinositol- specific phospholipase C. Biochemistry 47, 4201-4210.
14. Shi, X., Shao, C., Zhang, X., Zambonelli, C., Redfield, A. G., Head, J. F., Seaton, B. A., and Roberts, M. F. (2009) Modulation of Bacillus thuringiensis phosphatidylinositol-specific phospholipase C activity by mutations in the putative dimerization interface. J. Biol. Chem. 284, 15607-15618.
15. Pu, M., Fang, X., Redfield, A. G., Gershenson, A., and Roberts, M. F. (2009) Correlation of vesicle binding and phospholipid dynamics with phospholipase C activity: insights into phosphatidylcholine activation and surface dilution inhibition. J. Biol. Chem. 284, 16099-16107.
16. Gasteiger, E., Hoogland, C., Gattiker, A., Duvaud, S., Wilkins, M. R., Appel, R. D., and Bairoch, A. (2005) Protein identification and analysis tools on the ExPASy server, in The Proteomics Protocols Handbook (Walker, J. M., Ed.) pp 571-607, Humana Press, Totowa, NJ.
17. Volwerk, J. J., Shashidhar, M. S., Kuppe, A., and Griffith, O. H. (1990) Phosphatidylinositol-specific phospholipase C from Bacillus cereus combines intrinsic phosphotransferase and cyclic phosphodiesterase activities: a phosphorus-31 NMR study. Biochemistry 29, 8056-8062.
18. Liu, L., Mushero, N., Hedstrom, L., and Gershenson, A. (2006) Conformational distributions of protease-serpin complexes: a partially translocated complex. Biochemistry 45, 10865-10872.
19. Elson, E. L., and Magde, D. (1974) Fluorescence correlation spectroscopy. I. Conceptual basis and theory. Biopolymers 13, 1-27.
20. Takakuwa, Y., Pack, C. G., An, X. L., Manno, S., Ito, E., and Kinjo, M. (1999) Fluorescence correlation spectroscopy analysis of the hydrophobic interactions of protein 4.1 with phosphatidylserine liposomes. Biophys. Chem. 82, 149-155.
21. Thompson, N. L. (1991) Fluorescence correlation spectroscopy, in Topics in Fluorescence Spectroscopy (Lakowicz, J. R., Ed.) pp 337-378, Plenum Press, New York.
22. Magde, D., Elson, E. L., and Webb, W. W. (1974) Fluorescence correlation spectroscopy. II. An experimental realization. Biopolymers 13, 29-61.
23. Goñi, F., and Alonso, A. (2000) Membrane fusion induced by phospholipase C and sphingomyelinases. Biosci. Rep. 20, 443-463.
24. Wehbi, H., Feng, J., Kolbeck, J., Ananthanarayanan, B., Cho, W., and Roberts, M. F. (2003) Investigating the interfacial binding of bacterial phosphatidylinositol-specific phospholipase C. Biochemistry 42, 9374-9382.
25. Gässler, C. S., Ryan, M., Liu, T., Griffith, O. H., and Heinz, D. W. (1997) Probing the roles of active site residues in phosphatidylinositol- specific phospholipase C from Bacillus cereus by site-directed mutagenesis. Biochemistry 36, 12802-12813.
26. Hondal, R. J., Zhao, Z., Kravchuk, A. V., Liao, H., Riddle, S. R., Yue, X., Bruzik, K. S., and Tsai, M.-D. (1998) Mechanism of phosphatidylinositol- specific phospholipase C: a unified view of the mechanism of catalysis. Biochemistry 37, 4568-4580.
27. Viguera, A. R., and Serrano, L. (1999) Stable proline box motif at the N-terminal end of R-helices. Protein Sci. 8, 1733-1742.