Modulation of Bacillus thuringiensis phosphatidylinositolspecific phospholipase C activity by mutations in the putative dimerization interface

Journal of Biological Chemistry

Volumn 284, Issue 23, 2009, Pages 15607-15618

  Shi, Xiaomeng ^a   Shao, Chenghua ^b   Zhang, Xin ^a   Zambonelli, Carlo ^a   Redfield, Alfred G ^c   Head, James F ^b   Seaton, Barbara A ^b   Roberts, Mary F ^a  

^a BOSTON COLLEGE   (United States)

^b BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c BRANDEIS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; ASSAY SYSTEM; BACILLUS THURINGIENSIS; CATALYTIC ACTIVITY; CRYSTALLOGRAPHIC ANALYSIS; DIMER INTERFACE; ENZYMATIC ACTIVITIES; ENZYME BINDING; ENZYME FUNCTIONS; KINETIC ASSAY; LIPID BINDING; MEMBRANE BINDING; MUTANT PROTEINS; MYO-INOSITOL; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLINOSITOL; PHOSPHOLIPASE; PHOSPHOLIPASE C; PHOSPHOLIPID BINDING; PROTEIN DIMERIZATION; TYROSINE RESIDUES;

ALCOHOLS; AMINO ACIDS; ASSAYS; BACTERIOLOGY; BINDING ENERGY; DIMERIZATION; ENZYME ACTIVITY; ENZYMES; HYDROLYSIS; MONOMERS; PHOSPHOLIPIDS; PLANTS (BOTANY); SUGARS;

BINDING SITES;

DIMER; INOSITOL; MONOMER; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLINOSITOL 4,5 BISPHOSPHATE PHOSPHODIESTERASE; PHOSPHOLIPID; TYROSINE; BACTERIAL PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; BINDING SITE; CONTROLLED STUDY; CRYSTAL STRUCTURE; DIMERIZATION; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN SECONDARY STRUCTURE; STRUCTURE ANALYSIS; THERMOSTABILITY; AMINO ACID SUBSTITUTION; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; MUTATION; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; SPECTROFLUOROMETRY; X RAY CRYSTALLOGRAPHY;

BACILLUS THURINGIENSIS;

AMINO ACID SUBSTITUTION; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOSPHOINOSITIDE PHOSPHOLIPASE C; PROTEIN BINDING; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SPECTROMETRY, FLUORESCENCE; TYROSINE;

EID: 67650123206     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M901601200     Document Type: Article

References (45)

1. Heinz, D. W., Essen, L. O., and Williams, R. L. (1998) J. Mol. Biol. 275, 635-650
2. Rebecchi, M. J., and Pentyala, S. N. (2000) Physiol. Rev. 80, 1291-1335
3. Toker, A. (2002) Cell. Mol. Life Sci. 59, 761-779
4. Zhou, C., Wu, Y., and Roberts, M. F. (1997) Biochemistry 36, 347-355
5. Zhou, C., Qian, X., and Roberts, M. F. (1997) Biochemistry 36, 10089-10097
6. Qian, X., Zhou, C., and Roberts, M. F. (1998) Biochemistry 37, 6513-6522
7. Feng, J., Wehbi, H., and Roberts, M. F. (2002) J. Biol. Chem. 277, 19867-19875
8. Feng, J., Bradley, W. D., and Roberts, M. F. (2003) J. Biol. Chem. 278, 24651-24657
9. Shao, C., Shi, X., Wehbi, H., Zambonelli, C., Head, J. F., Seaton, B. A., and Roberts, M. F. (2007) J. Biol. Chem. 282, 9228-9235
10. Heinz, D. W., Ryan, M., Bullock, T. L., and Griffith, O. H. (1995) EMBO J. 14, 3855-3863
11. Wehbi, H., Feng, J., Kolbeck, J., Ananthanarayanan, B., Cho, W., and Roberts, M. F. (2003) Biochemistry 42, 9374-9382
12. Böhm, G., Muhr, R., and Jaenicke, R. (1992) Protein Eng. 5, 191-195
13. Andrade, M. A., Chacón, P., Merelo, J. J., and Morán, F. (1993) Protein Eng. 6, 383-390
14. Zhang, X., Wehbi, H., and Roberts, M. F. (2004) J. Biol. Chem. 279, 20490-20500
15. Roberts, M. F., Cui, Q., Turner, C. J., Case, D. A., and Redfield, A. G. (2004) Biochemistry 43, 3637-3650
16. Roberts, M. F., and Redfield, A. G. (2004) J. Am. Chem. Soc. 126, 13765-13777
17. Roberts, M. F., and Redfield, A. G. (2004) Proc. Natl. Acad. Sci. U. S. A. 101, 17066-17071
18. Lipari, G., and Szabo, A. (1982) J. Am. Chem. Soc. 104, 4546-4559
19. Herzfeld, J., Griffin, R. G., and Haberkorn, R. A. (1978) Biochemistry 17, 2711-2718
20. Woessner, D. E. (1995) in Encyclopedia of NMR (Grant, D. M., ed.) pp. 1068-1083, John Wiley & Sons, Inc., New York
21. Klauda, J. B., Roberts, M. F., Redfield, A. G., Brooks, B. R., and Pastor, R. W. (2008) Biophys. J. 94, 3074-3083
22. Otwinowski, Z., and Minor, W. (1997) Methods Enzymol. 276, 306-326
23. Kissinger, C. R., Gehlhaar, D. K., and Fogel, D. B. (1999) Acta Crystallogr. D Biol. Crystallogr. 55, 484-491
24. Jones, T. A., and Kjeldgaard, M. (1992) O: The Manual, Uppsala Software Factory, Uppsala, Sweden
25. Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. D Biol. Crystallogr. 54, 905-921
26. DeLano, W. L. (2002) The PyMOL Molecular Graphics System, DeLano Scientific, San Carlos, CA
27. Tsou, L. K., Tatko, C. D., and Waters, M. L. (2002) J. Am. Chem. Soc. 124, 14917-14921
28. Wu, Y., and Roberts, M. F. (1997) Biochemistry 36, 8514-8521
29. Wehbi, H., Feng, J., and Roberts, M. F. (2003) Biochim. Biophys. Acta 1613, 15-27
30. Volwerk, J. J., Shashidhar, M. S., Kuppe, A., and Griffith, O. H. (1990) Biochemistry 29, 8056-8062
31. Zhou, C., and Roberts, M. F. (1998) Biochemistry 37, 16430-16439
32. Bian, J., and Roberts, M. F. (1992) J. Colloid Interface Sci. 153, 420-428
33. Berg, O. G., Yu, B. Z., Apitz-Castro, R. J., and Jain, M. K. (2004) Biochemistry 43, 2080-2090
34. Lin, T. L., Chen, S. H., Gabriel, N. E., and Roberts, M. F. (1987) J. Phys. Chem. 91, 406-413
35. Barrall, G. A., Schmidt-Rohr, K., Lee, Y. K., Landfester, K., Zimmermann, H., Chingas, G. C., and Pines, A. (1995) J. Chem. Phys. 104, 509-520
36. Lin, T. L., Chen, S. H., Gabriel, N. E., and Roberts, M. F. (1986) J. Am. Chem. Soc. 108, 3499-3507
37. Sopkova-De Oliveira Santos, J., Fischer, S., Guilbert, C., Lewit-Bentley, A., and Smith, J. C. (2000) Biochemistry 39, 14065-14074
38. Ghosh, A. K., Rukmini, R., and Chattopadhyay, A. (1997) Biochemistry 36, 14291-14305
39. Han, S. K., Kim, K. P., Koduri, R., Bittova, L., Munoz, N. M., Leff, A. R., Wilton, D. C., Gelb, M. H., and Cho, W. (1999) J. Biol. Chem. 274, 11881-11888
40. Wallace, B. A., and Janes, R. W. (1999) Adv. Exp. Med. Biol. 467, 789-799
41. Hong, H., Park, S., Jiménez, R. H., Rinehart, D., and Tamm, L. K. (2007) J. Am. Chem. Soc. 129, 8320-8327
42. Schiffer, M., Chang, C. H., and Stevens, F. J. (1992) Protein Eng. 5, 213-214
43. Safferling, M., Griffith, H., Jin, J., Sharp, J., De Jesus, M., Ng, C., Krulwich, T. A., and Wang, D. N. (2003) Biochemistry 42, 13969-13976
44. Jain, M. K., and Berg, O. G. (1989) Biochim. Biophys. Acta 1002, 127-156
45. Gelb, M. H., Min, J. H., and Jain, M. K. (2000) Biochim. Biophys. Acta 1488, 20-27