Thymidine kinase and uridine-cytidine kinase from Entamoeba histolytica: Cloning, characterization, and search for specific inhibitors

Parasitology

Volumn 136, Issue 6, 2009, Pages 595-602

  Lossani, A ^a   Torti, A ^a   Gatti, S ^b   Bruno, A ^b   Maserati, R ^b   Wright, G E ^c   Focher, F ^a  

^a CNR   (Italy)

^b FONDAZIONE IRCCS POLICLINICO SAN MATTEO   (Italy)

^c GLSYNTHESIS INC   (United States)

Author keywords

Amoebiasis; Drugs; Entamoeba; Inhibitors; Kinases

Indexed keywords

2 PHENYLAMINO 9 (4 HYDROXYBUTYL) 6 OXOPURINE; 3' ETHYL 5 METHYL 2' DEOXYCYTIDINE; 5 PROPYLDEOXYURIDINE; 6 METHYLURIDINE; ADENOSINE TRIPHOSPHATE; ALPHA DEXTRO 5 ETHYL 2' DEOXYURIDINE; ALPHA THYMIDINE; BETA DEXTRO 5 ETHYL 2' DEOXYURIDINE; CHLOROQUINE; DEXTRO 5 (BROMOVINYL) 2' DEOXYURIDINE; EMETINE; ENZYME INHIBITOR; IDOXURIDINE; LEVO 5 (BROMOVINYL) 2' DEOXYURIDINE; METRONIDAZOLE; NITAZOXANIDE; NUCLEOSIDE ANALOG; PENCICLOVIR; TELBIVUDINE; THYMIDINE KINASE; TRIFLURIDINE; UNCLASSIFIED DRUG; URIDINE KINASE; ANTIPROTOZOAL AGENT; PROTOZOAL PROTEIN; RECOMBINANT PROTEIN;

AMEBIASIS; ARTICLE; CELL CULTURE; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENANTIOSELECTIVITY; ENTAMOEBA HISTOLYTICA; ENZYME ANALYSIS; ENZYME INHIBITION; ENZYME PHOSPHORYLATION; ENZYME SUBSTRATE; INTESTINE PARASITE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; CYTOLOGY; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; GENETICS; HUMAN; METABOLISM; MOLECULAR GENETICS; SEQUENCE ALIGNMENT;

ENTAMOEBA; ENTAMOEBA HISTOLYTICA; PROTOZOA;

AMINO ACID SEQUENCE; ANIMALS; ANTIPROTOZOAL AGENTS; CELL PROLIFERATION; CLONING, MOLECULAR; ENTAMOEBA HISTOLYTICA; HUMANS; MOLECULAR SEQUENCE DATA; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; SEQUENCE ALIGNMENT; THYMIDINE KINASE; URIDINE KINASE;

EID: 67651165418     PISSN: 00311820     EISSN: 14698161     Source Type: Journal    
DOI: 10.1017/S0031182009005964     Document Type: Article

References (21)

1. Bansal, D., Sehgal, R., Chawla, Y., Mahajan, R. C. and Malla, N. (2004). In vitro activity of antiamoebic drugs against clinical isolates of Entamoeba histolytica and Entamoeba dispar. Annals of Clinical Microbiology and Antimicrobials 21, 3-27. doi: 10.1186/1476-0711-3-27. (Pubitemid 44650230)
2. Bansal, D., Malla, N. and Mahajan, R. C. (2006). Drug resistance in amoebiasis. Indian Journal of Medical Research 123, 115-118.
3. Gatti, S., Swierczynski, G., Robinson, F., Anselmi, M., Corrales, J., Moreira, J., Montalvo, G., Bruno, A., Maserati, R., Bisoffi, Z. and Scaglia, M. (2002). Amebic infections due to the Entamoeba histolytica- Entamoeba dispar complex: a study of the incidence in a remote rural area of Ecuador. American Journal of Tropical Medicine and Hygiene 67, 123-127. (Pubitemid 35034332)
4. Leitsch, D., Kolarich, D., Wilson, I. B. H., Altmann, F. and Duchene, M. (2007). Metronidazole action in Entamoeba histolytica: a central role for thioredoxin reductase. PLoS Biology 5, 1820-1834.
5. Loftus, B., Anderson, I., Davies, R., Alsmark, U. C., Samuelson, J., Amedeo, P., Roncaglia, P., Berriman, M., Hirt, R. P., Mann, B. J., Nozaki, T., Suh, B., Pop, M., Duchene, M., Ackers, J., Tannich, E., Leippe, M., Hofer, M., Bruchhaus, I., Willhoeft, U., Bhattacharya, A., Chillingworth, T., Churcher, C., Hance, Z., Harris, B., Harris, D., Jagels, K., Moule, S., Mungall, K., Ormond, D., Squares, R., Whitehead, S., Quail, M. A., Rabbinowitsch, E., Norbertczak, H., Price, C., Wang, Z., Guillén, N., Gilchrist, C., Stroup, S. E., Bhattacharya, S., Lohia, A., Foster, P. G., Sicheritz-Ponten, T., Weber, C., Singh, U., Mukherjee, C., El-Sayed, N. M., Petri, W. A. Jr, Clark, C. G., Embley, T. M., Barrell, B., Fraser, C. M. and Hall, N. (2005). The genome of the protist parasite Entamoeba histolytica. Nature, London 433, 865-868. doi: 10.1038/nature03291.
6. Makioka, A., Kumagai, M., Kobayashi, S. and Takeuchi, T. (2002). Inhibition of excystation and metacystic development of Entamoeba invadens by the dinitroaniline herbicide oryzalin. Journal of Parasitology 88, 994-999. (Pubitemid 35278750)
7. Maga, G., Spadari, S., Wright, G. E. and Focher, F. (1994). Identification, partial purification and inhibition by guanine analogues of a novel enzymic activity which phosphorylates guanosine to GMP in the protozoan parasite Eimeria tenella. The Biochemical Journal 298, 289-294. (Pubitemid 24072468)
8. Ranque, S., Molet, B., Christmann, D. and Candolfi, E. (2004). In vitro activity of azithromycin and dirithromycin against axenic Entamoeba histolytica. European Journal of Clinical Microbiology & Infectious Diseases 23, 932-933. doi: 10.1007/s10096-004-1250-1. (Pubitemid 40104754)
9. Rossignol, J. F., Kabil, S. M., El-Gohary, Y. and Younis, A. M. (2007). Nitazoxanide in the treatment of amoebiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 101, 1025-1031. doi: 10.1016/j.trstmh.2007.04. 001.
10. Roy, B., Verri, A., Lossani, A., Spadari, S., Focher, F., Aubertin, A. M., Gosselin, G., Mathé, C. and Périgaud, C. (2004). Enantioselectivity of ribonucleotide reductase: a first study using stereoisomers of pyrimidine 2'-azido-2'deoxynucleosides. Biochemical Pharmacology 68, 711-718. doi: 10.1016/j.bcp.2004.05.002. (Pubitemid 38953452)
11. Samarawickrema, N. A., Brown, D. M., Upcroft, J. A., Thammapalerd, N. and Upcroft, P. (1997). Involvement of superoxide dismutase and pyruvate:ferredoxin oxidoreductase in mechanisms of metronidazole resistance in Entamoeba histolytica. Journal of Antimicrobial Chemotherapy 40, 833-840. (Pubitemid 28022148)
12. Sargeaunt, P. G., Williams, J. E. and Grene, J. D. (1978). The differentiation of invasive and non-invasive Entamoeba histolytica by isoenzyme electrophoresis. Transactions of the Royal Society of Tropical Medicine and Hygiene 72, 519-520.
13. Seifert, K., Duchene, M., Wernsdorfer, W. H., Kollaritsch, H., Scheiner, O., Wiedermann, G., Hottkowitz, T. and Eibl, H. (2001). Effects of miltefosine and other alkylphosphocholines on human intestinal parasite Entamoeba histolytica. Antimicrobial Agents and Chemotherapy 45, 1505-1510. doi: 10.1128/AAC.45.5.1505-1510.2001.
14. Spadari, S., Maga, G., Focher, F., Ciarrocchi, G., Manservigi, R., Arcamone, F., Capobianco, M., Carcuro, A., Colonna, F., Iotti, S. and Garbesi, A. (1992). L-thymidine is phosphorylated by Herpes simplex virus type 1 thymidine kinase and inhibits viral growth. Journal of Medicinal Chemistry 35, 4214-4220.
15. Spadari, S., Maga, G., Verri, A. and Focher, F. (1998). Molecular basis for the antiviral and anticancer activities of unnatural L-β-nucleosides. Expert Opinion on Investigational Drugs 7, 1285-1300. (Pubitemid 28359493)
16. Stanley, S. L. Jr. (2003). Amoebiasis. Lancet 361, 1025-1034.
17. Striepen, B., Pruijssers, A. J. P., Huang, J., Li, C., Gubbels, M.-J., Umejiego, N. N., Hedstrom, L. and Kissinger, J. C. (2004). Gene transfer in the evolution of parasite nucleoside biosynthesis. Proceedings of the National Academy of Sciences, USA 101, 3154-3159. doi: 10.1073/pnas.0304686101. (Pubitemid 38327750)
18. Strosselli, S., Spadari, S., Walker, R. T., Basnak, I. and Focher, F. (1998). Trichomonas vaginalis thymidine kinase: purification, characterization and search for inhibitors. The Biochemical Journal 334, 15-22. (Pubitemid 28420965)
19. Suzuki, N. N., Koizumi, K., Fukushima, M., Matsuda, A. and Inagaki, F. (2004). Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase. Structure 12, 751-764. doi: 10.1016/j.cell.2006.11. 023. (Pubitemid 38595764)
20. Wassmann, C., Hellberg, A., Tannich, E. and Bruchhaus, I. (1999). Metronidazole resistance in the protozoan parasite Entamoeba histolytica is associated with increased expression of iron-containing superoxide dismutase and peroxiredoxin and decreased expression of ferredoxin 1 and flavin reductase. Journal of Biological Chemistry 274, 26051-26056. (Pubitemid 129520027)
21. World Health Organization (1997). A consultation with experts on amoebiasis. Epidemiological Bulletin 18, 13-14.