Effect of lysis strategy in accuracy and repeatability of sex determination by single cell polymerase chain reaction method

Journal of Biological Sciences

Volumn 9, Issue 1, 2009, Pages 78-82

  Farzaneh Dehkordi, F ^a   Ahadi, A M ^b   Shirazi, A ^b   Sadeghizade, M ^c  

^a ISLAMIC AZAD UNIVERSITY   (Iran)

^b SHAHREKORD UNIVERSITY   (Iran)

^c TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

Cell lysis; Sex determination; Single cell PCR

Indexed keywords

DITHIOTHREITOL; DNA; NITROGEN; POTASSIUM HYDROXIDE; PROTEINASE K; TRITON X 114;

ACCURACY; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; BLASTOMERE; CONTROLLED STUDY; COW; CYTOLYSIS; DEVELOPMENTAL STAGE; DNA EXTRACTION; EMBRYO; EMBRYO DEVELOPMENT; FREEZE THAWING; GENE AMPLIFICATION; INTERMETHOD COMPARISON; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SEX DETERMINATION;

BOVINAE;

EID: 67650652273     PISSN: 17273048     EISSN: 18125719     Source Type: Journal    
DOI: 10.3923/jbs.2009.78.82     Document Type: Article

References (27)

1. Berezovski, M.V., T.W. Mak and S.N. Kryloy, 2007. Cell lysis inside the capillary facilitated by transverse diffusion of laminar flow profiles (TDLFP). Bioanal. Chem., 387:91-96.
2. Bredbackra, P. and O.Y. Finnzymes, 2001. Progress on methods of gene detection in preimplantation embryos. Theriogenology, 55: 23-34.
3. Brown, R.B. and J. Audet, 2008. Current techniques for single-cell lysis. J. R. Soc. Interface, 5: 131-138.
4. Chen, C.M., C.L. Hu, C.H. Wang, C.M. Hung and H.K. Wu et al., 1999. Gender determination in single bovine blastomeres by polymerase chain reaction amplification of sex-specific polymorphic fragments in the amelogenin gene. Mol. Reprod. Dev., 54: 209-214.
5. Chrenek, P., L. Boulanger, Y. Heyman, P. Uhrin, J. Laurinick, J. Bulla and J.P. Renard, 2001. Sexing and multiple genotype analysis from a single cell of bovine embryo. Theriogenology, 55: 1071-1081.
6. Di-Carlo, D., K.H. Jeong and L.P. Lee, 2003. Reagentless mechanical cell lysis by nanoscale barbs in microchannels for sample preparation. Lab. Chip, 3: 287-291.
7. Di-Carlo, D., C. Ionescu-Zanetti, Y. Zhang, P. Hung and L.P. Lee, 2005. On-chip cell lysis by local hydroxide generation. Lab. Chip. 5: 171-178.
8. Grifo, J.A., Y.X. Tang and J. Cohen, 1992. Pregnancy after embryo biopsy and coamplification of DNA from X and Y chromosornes. J. Am. Med. Assoc., 268: 727-729.
9. Handyside, A.K. E.H. Kontogianni and K. Handy, 1990. Pregnancies from biopsied human preimplantation embryos sexed by Y specific DNA amplification. Nature, 344: 768-770.
10. Hardy, K., R.M.L. Winston and A.H. Handyside, 1993. Binucleate blastomeres in human preimplantation embryos in vitro: Failure of cytokinesis during early cleavage. J Reprod. Fertil, 98: 549-558.
11. Hasler, J.F., E. Cardey, J.E. Stokes and P. Bradbacka, 2002. Nonelectrophoretic PCR-sexing of bovine embryos: in a commercial environment. Theriogenology, 58: 1457-1469.
12. Hussey. N.D., H. Donggue and D.A. Froiland, 1999. Analysis of five duchenne muscular dystrophy exones and gender determination using conventional duplex polymerase chain reaction on single cells. Mol. Hum. Reprod., 5: 1089-1094.
13. Kontogianni, E.H., D.X. Griffin and A.H. Handyside, 1996. Identifying the sex of human preimplantation embryos in X-linked disease: Amplification efficiency of Y-specific alphoid repeat from single blastomeres with two lysis protocols. J. Assisted Reprod. Genet., 13: 125-132.
14. Lee, J.H., J.H. Park, Lee, S.H. Park and D.I. Jim, 2004. Sexing using single blastomere derived from IVF bovine embryos by flourescence in situ hybridization (FISH). Theriogenology, 62: 1452-1458.
15. Lu, K.Y., A.M. Wo, Y.J. Lo, K.C. Chen, C.M. Lin and C.R. Yang, 2006. Three dimensional electrode array for cell lysis via electroporation. Biosens. Bioelectron, 22: 568-574.
16. Machaty, Z., Paldi, T. Csaki, Z. Varga, I. Kiss, Z. Barandi and G.. Vajta, 1993. Biopsy and sex determination by PCR of IVF bovine embryos. J. Reprod. Fertil., 98: 467-470.
17. Marc, P.J., C.E. Sims and N.L. Allbritton, 2007. Coaxial flow system for chemical cytometry. Anal. Chem., 79: 9054-9059.
18. Park, J.H., J.H. Lee, K.M. Choi, S.Y. Joung and J.Y. Kim et al., 2001. Rapid sexing of preimplantation bovine embryo using consecutive and multiplex polymerase chain reaction with biopsied single blastomere. Theriogenology, 55: 1843-1853.
19. Pierce, E.K., J.E. Rire, J.A. Sanchez and J. Lawrence, 2002. QuantiLyse: Reliable DNA amplification from single cells. Biotechniques, 32: 1106-1111.
20. Ramalho, M.F.P.D.T., J.M. Garciaa, C.R. Esper, R. Vantini and B.C.A. Alves et al., 2004. Sexing of murine and bovine embryos by developmental arrest induced by high-titer H-Y antisera. Theriogenology, 62: 1569-1576.
21. Ray, F.P., M. Vekemans and A. Munnich, 2001. Single cell multiplex PCR amplification of five dystrophin gene exones combined with gender determination. Mol. Hum. Reprod., 7: 489-494.
22. Schwers, S., A. Ehlich, & Kobsch, A. Missel and D. Loffert, 2000. Single-cell PCR and RT-PCR amplification specificity. QIAGEN News, 3: 14-18.
23. Thornhill, A., 2007. Single Cell Diagnostics: Methods and Protocols. Humana Press Inc., Totowa, New Jersey.
24. Tsuchiya, S., K. Sueoka, N. Matsuda, R. Tanigaki and H. Asada et al., 2005. The spanning protocol: A new DNA extraction method for efficient single-cell genetic diagnosis. J. Assisted Reprod. Genet., 22: 407-411.
25. Weikard, R., C. Kuehn, R. Brunner, D. Roschlau, C. Pitra, P. Laurent and M. Schwerin, 2001. Sex determination in cattle based on simultaneous amplification of a new male-specific DNA sequence and an autosomal locus using the same primers. Mol. Reprod. Dev., 60: 13-19.
26. Zhang, H. and W. Jin, 2004. Determination of different forms of human interferon-gamma in single natural killer cells by capillary electrophoresis with on-capillary immunoreaction and laser-induced fluorescence detection. Electrophoresis, 25: 1090-1095.
27. Zhang, H. and W. Jin, 20006. Single-cell analysis by intracellular immuno-reaction and capillary electrophoresis with laser-induced fluorescence detection. J. Chromatogr. A, 1104: 346-351.