Cell lysis inside the capillary facilitated by transverse diffusion of laminar flow profiles (TDLFP)

Analytical and Bioanalytical Chemistry

Volumn 387, Issue 1, 2007, Pages 91-96

  Berezovski, Maxim V ^a,b   Mak, Tak W ^b   Krylov, Sergey N ^a  

^a YORK UNIVERSITY   (Canada)

^b PRINCESS MARGARET HOSPITAL   (Canada)

Author keywords

Cell lysis; Chemical cytometry; Single cell analysis; Transverse diffusion of laminar flow profiles

Indexed keywords

CHROMATOGRAPHIC ANALYSIS; CYTOLOGY; LAMINAR FLOW; MOLECULAR BIOLOGY; SURFACE ACTIVE AGENTS;

CHEMICAL CYTOMETRY; LAMINAR FLOW PROFILES; SINGLE CELL ANALYSIS; TRANSVERSE DIFFUSION;

CELLS;

GREEN FLUORESCENT PROTEIN; OCTOXINOL;

ANIMAL; ARTICLE; CAPILLARY ELECTROPHORESIS; CELL LINE; CHEMISTRY; CYTOPHOTOMETRY; ELECTROOSMOSIS; EUKARYOTIC CELL; GENETICS; METABOLISM; METHODOLOGY; MICROFLUIDICS;

ANIMALS; CELL LINE; CYTOPHOTOMETRY; ELECTROOSMOSIS; ELECTROPHORESIS, CAPILLARY; EUKARYOTIC CELLS; GREEN FLUORESCENT PROTEINS; MICROFLUIDICS; OCTOXYNOL;

EID: 33845656089     PISSN: 16182642     EISSN: 16182650     Source Type: Journal    
DOI: 10.1007/s00216-006-0866-7     Document Type: Article

References (19)

1. Krylov SN et al (1999) Correlating cell cycle with metabolism in single cells: combination of image and metabolic cytometry, Cytometry 37:14-20
2. Zhang Z et al (2000) One-dimensional protein analysis of an HT29 human colon adenocarcinoma cell. Anal Chem72:318-322
3. Krylov SN et al (2000) Instrumentation for chemical cytometry. Anal Chem 72(4):872-878
4. Edstrom JE (1953) Nucleotide analysis on the cyto-scale. Nature 172:908
5. Dovichi NJ, Hu S (2003) Chemical cytometry. Curr Opin Chem Biol 7(5):603-608
6. McClain MA et al (2003) Microfluidic devices for the high-throughput chemical analysis of cells. Anal Chem 75(21):5646-5655
7. Wu H, Wheeler A, Zare RN (2004) Chemical cytometry on a picoliter-scale integrated microfluidic chip. Proc Natl Acad Sci USA 101(35):12809-12813
8. Hu K, Ahmadzadeh H, Krylov SN (2004) Asymmetry between sister cells in a cancer cell line revealed by chemical cytometry. Anal Chem 76(13):3864-3866
9. Sims CE et al (1998) Laser-micropipet combination for single-cell analysis. Anal Chem 70(21):4570-4577
10. Arkhipov SN et al (2005) Chemical cytometry for monitoring metabolism of a ras-mimicking substrate in single cells. Cytometry 63A(1):41-47
11. Healy KE, Lom B, Hockberger PE (1994) Spatial distribution of mammalian cells dictated by material surface chemistry. Biotech Bioeng 43:792-800
12. Krylov SN, Dovichi NJ (2000) Single-cell analysis using capillary electrophoresis: Influence of surface support properties on cell injection into the capillary. Electrophoresis 21:767-773
13. Tlsty TD (1998) Cell-adhesion-dependent influences on genomic instability and carcinogenesis. Curr Opin Cell Biol 10(5):647-653
14. Xue Q, Yeung ES (1996) Determination of lactate dehydrogenase isoenzymes in single lymphocytes from normal and leukemia cell lines. J Chromatogr B Biomed Appl 677(2):233-240
15. Han F et al (2003) Fast electrical lysis of cells for capillary electrophoresis. Anal Chem 75 (15):3688-3696
16. Xue Q, Yeung ES (1994) Variability of intracellular lactate dehydrogenase isoenzymes in single human erythrocytes. Anal Chem 66(7):1175-1178
17. Kristensen HK, Lau YY, Ewing AG (1994) Capillary electrophoresis of single cells: observation of two compartments of neurotransmitter vesicles. J Neurosci Methods 51:183-188
18. Okhonin V, Liu X, Krylov SN (2005) Transverse diffusion of laminar flow profiles to produce capillary nanoreactors. Anal Chem 77 5925-5929
19. Taylor JA, Yeung ES (1993) Imaging of hydrodynamic and electrokinetic flow profiles in capillaries. Anal Chem 65:2928-2932