Antioxidant activity and quantitative estimation of azadirachtin and nimbin in Azadirachta Indica A. Juss grown in foothills of Nepal

African Journal of Biotechnology

Volumn 8, Issue 13, 2009, Pages 3084-3091

  Ghimeray, Amal Kumar ^a   Jin, Cheng Wu ^a   Ghimire, Bimal Kumar ^a   Dong, Ha Cho ^a  

^a Kangwon National University   (South Korea)

Author keywords

Antioxidant; Azadirachta indica A. Juss (neem); Azadirachtin; Flavonoid; Foothills (subtropical region); HPLC; Nimbin; Polyphenol

Indexed keywords

1,1 DIPHENYL 2 PICRYLHYDRAZYL; ANTIOXIDANT; AZADIRACHTIN; FLAVONOID; HEXANE; HYDROXYL RADICAL; LINOLEIC ACID; METAL CHELATE; METHANOL; NIMBIN; QUERCETIN; SCAVENGER; TANNIN;

ANTIOXIDANT ACTIVITY; ARTICLE; AZADIRACHTA INDICA; BARK; CHELATION; CONTROLLED STUDY; DNA DAMAGE; EVALUATION RESEARCH; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIPID PEROXIDATION; NEPAL; NONHUMAN; PEROXIDATION; PLANT LEAF; QUANTITATIVE ANALYSIS;

AZADIRACHTA INDICA;

EID: 67650503905     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

1. Aruoma OI (1994). Deoxyribose assay for detecting hydroxyl radicals. Methods Enzymol. 233: 57-66. (Pubitemid 24177528)
2. Allan EJ, Stuchbury T, Mordue (Luntz) AJ (1999). Azadirachta indica A.Juss. (Neem tree): In vitro culture, micropropagation and the production of azadirachtin and other secondary metabolites. In: Bajaj YPS (Ed) Biotechnology in agriculture and forestry science series, Springer, Berlin Heidelberg NY, Medical aromatic plants, 43: 11-41.
3. Biswas KI, chattopadhyay R, Banerjee K, Bandyopadhyay U (2002). Biological activities and medicinal properties of Neem (Azadirachta indica). Curr. Sci. 82(11): 1336-1345.
4. Balasundram N, Sundram K, Samman S (2006). Phenolic com-pounds in plants and agri-industrial by-products: Antioxidant activity, occur rence, and potential uses. Food Chem. 99: 191-203.
5. Branen AL, Davidson PM (1997). Use of antioxidants in self pre-serving cosmetic and drug formulations. In: Preservative-Free and Self- Preserving Cosmetics and Drugs, Cosmetic Sci. Technol. Series, 16: 159-179.
6. Bracca A, Fico G, Morelli I, De Simone F, Tome F, De Tommasi N (2003). Antioxidant and free radical scavenging activity of flavonol glycosides from different Aconitum species. J. Ethnopharmacol. 86: 63-67. (Pubitemid 36398660)
7. +-ATPase inhibition and scavenging of hydroxyl radical. Life sci. 71: 2845-2865.
8. Bravo L (1998). Polyphenols: Chemistry, dietary sources, metabolism, and nutritional significance. Nutr. Rev. 56: 317-333.
9. Cai Y, Luo Q, Sun M, Corke H (2004). Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer. Life Sci. 74: 2157-2184. (Pubitemid 38210440)
10. Decker EA, Welch B (1990). Role of Ferritin as a lipid oxidation catalyst in muscle food. J. Agric. Food Chem. 38: 674-677.
11. Duh PD, Tu YY, Yen GC (1999). Antioxidant activity of water extract of Harn jyur (Chrysanthemum morifolium Ramat), Lebensmittel-Wissenschaft Technologie. 32: 269-277.
12. Eom SH, Cheng WJ, Hyoung JP, Kim EH, Chung MI, Kim MJ, Yu CY, Cho DH (2007). Far infrared ray irradiation stimulates antioxidant activity in Vitis flexuosa THUNB. Berries. Korean J. Med. Crop Sci. 15 (5): 319-323.
13. Ermel K (1995). Azadirachtin content of Neem seed kernels from different regions of the world. In: Schmutterer H (Ed.). The Neem tree, source of Unique Natural products for integrated pest management, medicine, industry and other purposes, VCH, Weinheim, pp. 89-92.
14. Ermel K, Pahlich E, Schmutterer H (1986). Azadirachtin contents of Neem kernels from different geographical locations and its dependence on temperature, relative humidity and light. In proceedings, international Neem conference, Nairobi, Kenya, pp. 171-184.
15. Finefrock AE, Bush AI, Doraiswamy PM (2003). Current status of metals as therapeutic targets in Alzheimer's disease. J. Am. Geriatr. Soc. 51: 1143-1148.
16. Gogati SS, Marathe AD (1989). Anti-viral effect of Neem leaf (Azadirachta indica) extracts on Chinkugunga and Measles viruses. J. Res. Edu. Ind. Med. 8: 1-5.
17. Hsu B, Coupar IM, Ng K (2006). Antioxidant activity of hot water extract from the fruit of the Doum palm, Hyphaene thebaica. Food Chem. 98(2): 317-328.
18. Halliwell B, Aeschbach R, Loliger J, Aruoma OI (1995). The characterization of antioxidants, Food Chem. Toxicol. 33(7): 601-617.
19. Kaura SK, Gupta SK, Chowdhury JB (1998). Morphological and oil content variation in seeds of Azadirachta indica A. Juss. (Neem) from northern and western provenances of India. Plant foods for human Nutr. 52: 293-298.
20. Kim KT, Yoo KM, Lee JW, Eom SH, Hwang IK, Lee CY (2007). Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharm. 111: 443-445.
21. Kher A, Chaurasia SC (1997). Antifungal activity of essential oils of three medical plants. Ind. Drug, 15: 41-42.
22. Kaushik N, Gurudev singh B, Tomar UK, Naik SN, Satya V, Bisla SS, Sharma SK, Banerjee SK, Thakkar P (2007). Regional and habitat variability in azadirachtin content of Indian Neem (Azadirachta indica A Juss.). Curr. Sci. 92(10):1400-1406.
23. Lloyd RV, Hanna PM, Mason RP (1997). The origin of the hydroxyl radical oxygen in the Fenton reaction. Free Rad. Biol. Med. 22(5):885-888.
24. Morton LW, Abu-Amsha Caccetta R, Puddey IB, Croft KD (2000). Chemistry and biological effects of dietary phenolic compounds: relevance to cardiovascular disease. Clin. Exp. Pharm. Phys. 27: 152-159.
25. Meir SJ, Kanner B, Akiri SPH (1995). Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J. Agric. Food Chem. 43: 1813-1817.
26. Okpanyi SN, Ezeukwu GC (1981). Anti-inflammatory and antipyretic activities of Azadirachta indica. Planta media, 4 (1): 34-39.
27. Pitchaon M, Suttajit M, Pongsawatmanit R (2007). Assessment of phenolic content and free radical-scavenging capacity of some Thai indigenous plants. Food Chem. 100: 1409-1418. (Pubitemid 44160748)
28. Pokorney J (2001). Introduction. In Pokorny J, Yanishlieva N, Gordon MH (Eds.), Antioxidants in food: practical applications, Cambridge: Woodhead Publishing Limited. pp. 1-3.
29. Qian ZJ, Jung WK, Byun HG, Kim SK (2008). Protective effect of an antioxidative peptide purified from gastrointestinal digests of oyster, Crassostrea gigas against free radical induced DNA damage. Biores. technol. 99: 3365-3371.
30. Sultana B, Anwar F, Przybylski R (2007). Antioxidant activity of phenolic components present in barks of Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam. trees. J. Food Chem. 104: 1106-1114. (Pubitemid 46764464)
31. Sombatsiri K, Ermel K, Schmutterer H (1995). Other Meliaceous plants containing ingredients for integrated pest management and further purpose. In H. Schmutter (Ed.), The Neem tree Azadirachta indica A. Juss and other meliaceous plants. Germany: VCH pp. 585-597
32. Siddiqui BS, Ghiasuddin Faizi S, Siddiqui S (1992). Triterpenoids from the fresh fruit coats of Azadirachta indica. Phytochem. 31(12): 4275-4278.
33. Singh N, Sastry MS (1997). Antimicrobial activity of Neem oil. Ind. J. Pharmaciol. 13: 102-106.
34. Sasaki S, Ohta T, Decker EA (1996). Antioxidant activity of water-soluble fractions of salmon spermary tissue. J. Agric. Food Chem. 44: 1682-1686.
35. Sidhu O, Vishal Kumar P, Hari MB (2004). Variability in triterpenoids (Nimbin and Salanin) composition of neem among different provenances of India. Ind. crops products, 19: 69-75. (Pubitemid 38039006)
36. Siddhuraju P, Mohan PS, Becker K (2002). Studies on the antioxidant activity of Indian Laburnum (Cassia fistula L.): A preliminary assessment of crude extracts from stem bark, leaves, flower and fruit pulp. Food Chem. 79: 61-67.
37. +ATPase and Helicobacter pylori Growth by Phenolic Antioxidants of Curcuma amada. J. Agric. Food Chem. 55(18): 7377-7386.
38. Soares JR, Dins TCP, Cunha AP (1997). Antioxidant activity of some extracts of Thymus zygis, Free Radical Res. 26: 469-478.
39. Strack D (1997). Phenolic metabolism. In Plant Biochemistry, Dey PM, Harborne JB Eds, Academic Press, San Diego, pp. 387-416.
40. Thompson M, Williams CR (1976). Stability of flavonoids complexes of copper (II) and flavonoid antioxidant activity. Anal. Chem. Acta. 85: 375-381.