The Rho-A/Rho-kinase pathway is up-regulated but remains inhibited by cyclic guanosine monophosphate-dependent mechanisms during endotoxemia in small mesenteric arteries

Critical Care Medicine

Volumn 37, Issue 5, 2009, Pages 1716-1723

  Da Silva Santos, J Eduardo ^a,b   Chiao, Chin Wei ^a   Leite, Romulo ^a   Webb, R Clinton ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

^b FEDERAL UNIVERSITY OF PARÁ   (Brazil)

Author keywords

Guanylate cyclase; Hypotension; Myosin phosphatase; Nitric oxide; Septic shock; Vascular hyporesponsiveness

Indexed keywords

4 (1 AMINOETHYL) N (4 PYRIDYL)CYCLOHEXANECARBOXAMIDE; CYCLIC GMP; GUANYLATE CYCLASE INHIBITOR; LIPOPOLYSACCHARIDE; MYOSIN PHOSPHATASE TARGETING SUBUNIT 1; N(G) NITROARGININE; NITRIC OXIDE; OXADIAZOLO[4,3 ALPHA]QUINOXALIN 1 ONE; PHOSPHATASE; RHO KINASE; RHO KINASE 1; RHO KINASE 2; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BLOOD VESSEL REACTIVITY; CONTROLLED STUDY; ENDOTHELIUM; ENDOTOXEMIA; ENZYME ACTIVITY; ENZYME PHOSPHORYLATION; INCUBATION TIME; MALE; MESENTERIC ARTERY; MYOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; SEPTIC SHOCK; UPREGULATION; WESTERN BLOTTING;

AMIDES; ANALYSIS OF VARIANCE; ANIMALS; BLOTTING, WESTERN; CYCLIC GMP; DISEASE MODELS, ANIMAL; ENDOTOXEMIA; LIPOPOLYSACCHARIDES; MALE; MESENTERIC ARTERIES; NITRIC OXIDE; PHOSPHORYLATION; PROBABILITY; PYRIDINES; RANDOM ALLOCATION; RATS; RATS, WISTAR; RHO-ASSOCIATED KINASES; SENSITIVITY AND SPECIFICITY; SIGNAL TRANSDUCTION; UP-REGULATION; VASOCONSTRICTION; VASODILATION;

EID: 67650480778     PISSN: 00903493     EISSN: 15300293     Source Type: Journal    
DOI: 10.1097/CCM.0b013e31819efb43     Document Type: Article

References (37)

1. Mitolo-Chieppa D, Serio M, Potenza MA, et al: Hyporeactivity of mesenteric vascular bed in endotoxin-treated rats. Eur J Pharmacol 1996; 309:175-182
2. Annane D, Trabold F, Sharshar T, et al: Inappropriate sympathetic activation at onset of septic shock: A spectral analysis approach. Am J Respir Crit Care Med 1999; 160: 458-465
3. Takakura K, Xiaohong W, Takeuchi K, et al: Deactivation of norepinephrine by peroxynitrite as a new pathogenesis in the hypotension of septic shock. Anesthesiology 2003; 98:928-934
4. Takakura K, Taniguchi T, Muramatsu I, et al: Modification of alpha1-adrenoceptors by per-oxynitrite as a possible mechanism of systemic hypotension in sepsis. Crit Care Med 2002; 30:894-899
5. Martinez MC, Muller B, Stoclet JC, et al: Alteration by lipopolysaccharide of the relationship between intracellular calcium levels and contraction in rat mesenteric artery. Br J Pharmacol 1996; 118:1218-1222
6. Mansart A, Bollaert PE, Giummelly P, et al: Effects of dexamethasone and L-canavanine on the intracellular calcium-contraction relation of the rat tail artery during septic shock. Am J Physiol Heart Circ Physiol 2006; 291:H1177-H1182
7. Wu CC, Chen SJ, Yen MH: Nitric oxideindependent activation of soluble guanylyl cyclase contributes to endotoxin shock in rats. Am J Physiol 1998; 275:H1148-H1157
8. + channels underlies relaxation to bacterial lipopolysaccharide in rat aorta. Biochem Biophys Res Commun 1996; 224: 184-190
9. Vanelli G, Hussain SN, Aguggini G: Glibenclamide, a pageer of ATP-sensitive potassium channels, reverses endotoxin-induced hypotension in pig. Exp Physiol 1995; 80:167-170
10. + pump in aortas from rats with endotoxaemia. Naunyn Schmiedebergs Arch Pharmacol 2003; 368:57-62
11. +-ATPase in the aorta from rats with endotoxic shock. Shock 2005; 23: 179-185
12. da Silva-Santos JE, Terluk MR, Assreuy J: Differential involvement of guanylate cyclase and potassium channels in nitric oxide-induced hyporesponsiveness to phenylephrine in endotoxemic rats. Shock 2002; 17: 70-76
13. Kokubu N, Satoh M, Takayanagi I: Involvement of botulinum C3-sensitive GTP-binding proteins in alpha 1-adrenoceptor subtypes mediating Ca(2+)-sensitization. Eur J Pharmacol 1995; 290:19-27
14. Nishimura J, Sakihara C, Zhou Y, et al: Ex- pression of rho A and rho kinase mRNAs in porcine vascular smooth muscle. Biochem Biophys Res Commun 1996; 227:750-754
15. Kimura K, Ito M, Amano M, et al: Regulation of myosin phosphatase by Rho and Rhoassociated kinase (Rho-kinase). Science 1996; 273:245-248
16. Ishizaki T, Maekawa M, Fujisawa K, et al: The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase. Embo J 1996; 15:1885-1893
17. Hilgers RH, Webb RC: Molecular aspects of arterial smooth muscle contraction: Focus on Rho. Exp Biol Med 2005; 230:829-835
18. Slotta JE, Braun OO, Menger MD, et al: Fa-sudil, a Rho-kinase inhibitor, inhibits leukocyte adhesion in inflamed large blood vessels in vivo. Inflamm Res 2006; 55:364-367
19. Thorlacius K, Slotta JE, Laschke MW, et al: Protective effect of fasudil, a Rho-kinase inhibitor, on chemokine expression, leukocyte recruitment, and hepatocellular apoptosis in septic liver injury. J Leukoc Biol 2006; 79: 923-931
20. Mills TM, Chitaley K, Lewis RW, et al: Nitric oxide inhibits RhoA/Rho-kinase signaling to cause penile erection. Eur J Pharmacol 2002; 439:173-174
21. Chitaley K, Webb RC: Nitric oxide induces dilation of rat aorta via inhibition of rhokinase signaling. Hypertension 2002; 39: 438-442
22. Teixeira EC, Jin L, Ying Z, et al: Expression and functional role of the RhoA/Rho-kinase pathway in rat celiac artery. Clin Exp Pharmacol Physiol 2005; 32:817-824
23. Davies SP, Reddy H, Caivano M, et al: Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 2000; 351:95-105
24. Szabo C, Southan GJ, Thiemermann C: Beneficial effects and improved survival in rodent models of septic shock with S-methylisothiourea sulfate, a potent and selective inhibitor of inducible nitric oxide synthase. Proc Natl Acad Sci USA 1994; 91: 12472-12476
25. Whitsett J, Martásek P, Zhao H, et al: Endothelial cell superoxide anion radical generation is not dependent on endothelial nitric oxide synthase-serine 1179 phosphorylation and endothelial nitric oxide synthase dimer/ monomer distribution. Free Radic Biol Med 2006; 40:2056-2068
26. Salvemini D, Cuzzocrea S: Therapeutic potential of superoxide dismutase mimetics as therapeutic agents in critical care medicine. Crit Care Med 2003; 31:S29-S38
27. Da Silva-Santos JE, Assreuy J: Long-lasting changes of rat blood pressure to vasoconstrictors and vasodilators induced by nitric oxide donor infusion: Involvement of potassium channels. J Pharmacol Exp Ther 1999; 290:380-387
28. Muller B, Kleschyov AL, Alencar JL, et al: Nitric oxide transport and storage in the cardiovascular system. Ann N Y Acad Sci 2002; 962:131-139
29. Alencar JL, Lobysheva I, Chalupsky K, et al: S-nitrosating nitric oxide donors induce long-lasting inhibition of contraction in isolated arteries. J Pharmacol Exp Ther 2003; 307:152-159
30. Feletou M, Vanhout PM: Endotheliumderived hyperpolarizing factor. Where are we now? Arterioscler Thromb Vasc Biol 2006; 26:1215-1225
31. Paya D, Gray GA, Stoclet JC: Effects of methylene blue on blood pressure and reactivity to norepinephrine in endotoxemic rats. J Cardiovasc Pharmacol 1993; 21:926-930
32. Cheng X, Pang CC: Pressor and vasoconstrictor effects of methylene blue in endotoxae-mic rats. Naunyn Schmiedebergs Arch Pharmacol 1998; 357:648-653
33. Keaney JF, Puyana JC, Francis S, et al: Methylene blue reverses endotoxin-induced hypotension. Circ Res 1994; 74:1121-1125
34. Fernandes D, da Silva-Santos JE, Duma D, et al: Nitric oxide-dependent reduction in soluble guanylate cyclase functionality accounts for early lipopolysaccharide-induced changes in vascular reactivity. Mol Pharmacol 2006; 69:983-990
35. Ichikawa K, Ito M, Hartshorne DJ: Phosphorylation of the large subunit of myosin phosphatase and inhibition of phosphatase activity. J Biol Chem 1996; 271:4733-4740
36. 2+ desensitization in vascular smooth muscle by activating the myosin light chain phosphatase. J Biol Chem 1997; 272: 5063-5068
37. Wooldridg AA, MacDonald JA, Erdodi F, et al: Smooth muscle phosphatase is regulated in vivo by exclusion of phosphorylation of threonine 696 of MYPT1 by phosphorylation of Serine 695 in response to cyclic nucleotides. J Biol Chem 2004; 279:34496-34504