CAMP-dependent chloride conductance evokes ammonia-induced blebbing in the microglial cell line, BV-2

Cellular Physiology and Biochemistry

Volumn 24, Issue 1-2, 2009, Pages 53-64

  Svoboda, Nina ^a   Pruetting, Sylvia ^b   Grissmer, Stephan ^b   Kerschbaum, Hubert H ^a  

^a UNIVERSITY OF SALZBURG   (Austria)

^b UNIVERSITY OF ULM   (Germany)

Author keywords

Ammonium; Apoptosis; cAMP; Cell blebbing; Chloride conductance; Microglia

Indexed keywords

5 NITRO 2 (3 PHENYLPROPYLAMINO)BENZOIC ACID; ADENYLATE CYCLASE; ALKANOIC ACID; AMMONIA; CHLORIDE CHANNEL; CHLORIDE ION; CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; FLUFENAMIC ACID;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL BLEBBING; CELL DAMAGE; CELL LINE; CELL VOLUME; CHLORIDE CONDUCTANCE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME INHIBITION; KINETICS; MICROGLIA; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; WHOLE CELL;

EID: 67650465601     PISSN: 10158987     EISSN: None     Source Type: Journal    
DOI: 10.1159/000227813     Document Type: Article

References (37)

1. Del Rio-Hortega P: Microglia; in Penfield W (ed): Cytology and cellular pathology of the nervous system. New York, PB Hoebaer, 1932, pp 483-534.
2. Kreutzberg GW: Microglia: a sensor for pathological events in the CNS. Trends Neurosci 1996;19:312-318.
3. Ladeby R, Wirenfeldt M, Garcia-Ovejero D, Fenger C, Dissing-Olesen L, Dalmau I, Finsen B: Microglial cell population dynamics in the injured adult central nervous system. Brain Res Brain Res Rev 2005;48:196-206.
4. Davalos D, Grutzendler J, Yang G, Kim JV, Zuo Y, Jung S, Littman DR, Dustin ML, Gan WB: ATP mediates rapid microglial response to local brain injury in vivo. Nat Neurosci 2005;8:752-758.
5. Nimmerjahn A, Kirchhoff F, Helmchen F: Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science 2005;308:1314-1318.
6. Franklin RJ: Why does remyelination fail in multiple sclerosis? Nat Rev Neurosci 2002;3:705-714.
7. Hauwel M, Furon E, Canova C, Griffiths M, Neal J, Gasque P: Innate (inherent) control of brain infection, brain inflammation and brain repair: the role of microglia, astrocytes, "protective" glial stem cells and stromal ependymal cells. Brain Res Rev 2005;48:220-233.
8. Eder C, Klee R, Heinemann U: Involvement of stretch-activated Cl- channels in ramification of murine microglia. J Neurosci 1998;15:7127-7137.
9. Furtner T, Zierler S, Kerschbaum HH: Blockade of chloride channels suppresses engulfment of microspheres in the microglial cell line, BV-2. Brain Res 2007;1184:1-9.
10. Svoboda N, Zierler S, Kerschbaum HH: cAMP mediates ammonia-induced programmed cell death in the microglial cell line, BV-2. Eur J Neurosci 2007;25:2285-2295.
11. Zierler S, Kerschbaum HH: Blockade of chloride conductance antagonizes PMA-induced ramification in the murine microglial cell line, BV-2. Brain Res 2005;1039:162-170.
12. Zierler S, Klein B, Furtner T, Bresgen N, Luetz-Meindl U, Kerschbaum HH: Ultraviolet irradiation-induced apoptosis does not trigger nuclear fragmentation but translocation of chromatin from nucleus into cytoplasm in the microglial cell-line, BV-2. Brain Res 2006;1121:12-21.
13. Zierler S, Frei E, Grissmer S, Kerschbaum HH: Chloride influx provokes lamellipodium formation in microglial cells. Cell Physiol Biochem 2008;21:55-62.
14. Carpenter E, Peers C: Swelling- and cAMP-activated Cl- currents in isolated rat carotid body type I cells. J Physiol 1997;503:497-511.
15. Meng XJ, Weinman SA: cAMP- and swelling-activated chloride conductance in rat hepatocytes. Am J Physiol 1996;271:C112-120.
16. Charras GT, Yarrow JC, Horton MA, Mahadevan L, Mitchison TJ: Non-equilibration of hydrostatic pressure in blebbing cells. Nature 2005;435:365-369.
17. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ: Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 1981;391:85-100.
18. Kerschbaum HH, Negulescu PA, Cahalan MD: Ion channels, Ca2+ signaling, and reporter gene expression in antigenspecific mouse T cells. J Immunol 1997;159:1628-1638.
19. Lewis RS, Ross PE, Cahalan MD: Chloride channels activated by osmotic stress in T lymphocytes. J Gen Physiol 1993;101:801-826.
20. Marcaggi P, Coles J: Ammonium in nervous tissue: transport across cell membranes, fluxes from neurons to glial cells, and role in signalling. Prog Neurobiol 2001;64:157-183.
21. Felipo V, Butterworth RF: Neurobiology of ammonia. Prog Neurobiol 2002;67:259-279.
22. 3 excretion from lungs? Pflugers Arch 2007;455(2):223-239.
23. Ducharme G, Newell EW, Pinto C, Schlichter LC: Small-conductance Cl-channels contribute to volume regulation and phagocytosis in microglia. Eur J Neurosci 2007;26:2119-2130.
24. Eder C: Regulation of microglial behavior by ion channel activity. J Neurosci Res 2005;81:314-321.
25. Lang F, Busch GL, Ritter M, Voelkl H, Waldegger S, Gulbins E, Haeussinger D: Functional significance of cell volume regulatory mechanisms. Physiol Rev 1998;78:247-306.
26. Charras GT, Coughlin M, Mitchison TJ, Mahadevan L: Life and times of a cellular bleb. Biophys J 2008;94:1836-1853.
27. Harris A: Cell surface movements related to cell locomotion. CIBA Found Symp 1973;14:3-20.
28. Cunningham CC: Actin polymerization and intracellular solvent flow in cell surface blebbing. J Cell Biol 1995;129:1589-1599.
29. Mills JC, Stone NL, Erhardt J, Pittman RN: Apoptotic membrane blebbing is regulated by myosin light chain phosphorylation. J Cell Biol 1998;140:627-636.
30. Eder C: Ion channels in microglia (brain macrophages). Am J Physiol 1998;275:C327-C342.
31. Rappert A, Biber K, Nolte C, Lipp M, Schubel A, Lu B, Gerard NP, Gerard C, Boddeke HW, Kettenmann H: Secondary lymphoid tissue chemokine (CCL21) activates CXCR3 to trigger a Cl- current and chemotaxis in murine microglia. J Immunol 2002;168:3221-3226.
32. Torgerson RR, McNiven MA: The actin-myosin cytoskeleton mediates reversible agonist-induced membrane blebbing. J Cell Sci 1998;111:2911-2922.
33. Baumgartner M, Patel H, Barber DL: Na(+)/H(+) exchanger NHE1 as plasma membrane scaffold in the assembly of signaling complexes. Am J Physiol Cell Physiol 2004;287(4):C844-C850.
34. Frantz C, Barreiro G, Dominguez L, Chen X, Eddy R, Condeelis J, Kelly MJ, Jacobson MP, Barber DL: Cofilin is a pH sensor for actin free barbed end formation: role of phosphoinositide binding. J Cell Biol 2008;183(5):865-879.
35. Beene DL, Scott JD: A-kinase anchoring proteins take shape. Curr Opin Cell Biol 2007;19:192-198.
36. Lascola CD, Kraig RP: Whole-cell chloride currents in rat astrocytes accompany changes in cell morphology. J Neurosci 1996;16:2532-2545.
37. Ullrich N, Sontheimer H: Cell cycle-dependent expression of a glioma-specific chloride current: proposed link to cytoskeletal changes. Am J Physiol 1997;273:C1290-C1297.