Site-directed spin-labeling study of the light-harvesting complex CP29

Biophysical Journal

Volumn 96, Issue 9, 2009, Pages 3620-3628

  Kavalenka, Aleh A ^a,b   Spruijt, Ruud B ^a   Wolfs, Cor J A M ^a   Štrancar, Janez ^b   Croce, Roberta ^c   Hemminga, Marcus A ^a   Van Amerongen, Herbert ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b JO EF STEFAN INSTITUTE   (Slovenia)

^c UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; APOPROTEIN; CYSTEINE; MEMBRANE PROTEIN; NITROXIDE; PROTEIN; THREONINE; ARABIDOPSIS PROTEIN; CAROTENOID; CP29 LIGHT HARVESTING COMPLEX;

ARTICLE; ELECTRON SPIN RESONANCE; ENZYME RECONSTITUTION; EVOLUTION; LIGHT HARVESTING SYSTEM; NONHUMAN; PHOTOSYNTHESIS; POLYMERIZATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; REGULATORY MECHANISM; SPIN LABELING; WILD TYPE; ARABIDOPSIS; BIOLOGICAL MODEL; CHEMISTRY; COMPUTER SIMULATION; ESCHERICHIA COLI; GENETICS; METABOLISM; MUTATION; PHOTOSYSTEM II; PROTEIN CONFORMATION; SITE DIRECTED MUTAGENESIS; SPINACH;

APOPROTEINS; ARABIDOPSIS; ARABIDOPSIS PROTEINS; CAROTENOIDS; COMPUTER SIMULATION; ELECTRON SPIN RESONANCE SPECTROSCOPY; ESCHERICHIA COLI; LIGHT-HARVESTING PROTEIN COMPLEXES; MODELS, BIOLOGICAL; MUTAGENESIS, SITE-DIRECTED; MUTATION; PHOTOSYSTEM II PROTEIN COMPLEX; PROTEIN CONFORMATION; SPINACIA OLERACEA;

EID: 67650394201     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2009.01.038     Document Type: Article

References (39)

1. Nelson, N., and C. F. Yocum. 2006. Structure and function of photosystems I and II. Annu. Rev. Plant Biol. 57:521-565.
2. Jansson, S. 1999. A guide to the Lhc genes and their relatives in Arabidopsis. Trends Plant Sci. 4:236-240.
3. Caffarri, S., R. Croce, L. Cattivelli, and R. Bassi. 2004. A look within LHCII: differential analysis of the Lhcb1-3 complexes building the major trimeric antenna complex of higher-plant photosynthesis. Biochemistry. 43:9467-9476.
4. Dekker, J. P., and E. J. Boekema. 2005. Supramolecular organization of thylakoid membrane proteins in green plants. Biochim. Biophys. Acta. 1706:12-39.
5. Avenson, T. J., T. K. Ahn, D. Zigmantas, K. K. Niyogi, Z. Li, et al. 2008. Zeaxanthin radical cation formation in minor light-harvesting complexes of higher plant antenna. J. Biol. Chem. 283:3550-3558.
6. Ahn, T. K., T. J. Avenson, M. Ballottari, Y.-C. Cheng, K. K. Niyogi, et al. 2008. Architecture of a charge-transfer state regulating light harvesting in a plant antenna protein. Science. 320:794-797.
7. Liu, Z., H. Yan, K. Wang, T. Kuang, J. Zhang, et al. 2004. Crystal structure of spinach major light-harvesting complex at 2.72 Å resolution. Nature. 428:287-292.
8. Green, B. R., and D. G. Durnford. 1996. The chlorophyll-carotenoid proteins of oxygenic photosynthesis. Annu. Rev. Plant Physiol. Plant Mol. Biol. 47:685-714.
9. Bassi, R., R. Croce, D. Cugini, and D. Sandonà. 1999. Mutational analysis of a higher plant antenna protein provides identification of chromophores bound into multiple sites. Proc. Natl. Acad. Sci. USA. 96:10056-10061.
10. Sandonà, D., R. Croce, A. Pagano, M. Crimi, and R. Bassi. 1998. Higher plants light harvesting proteins. Structure and function as revealed by mutation analysis of either protein or chromophore moieties. Biochim. Biophys. Acta. 1365:207-214.
11. Testi, M. G., R. Croce, P. P.-D. Laureto, and R. Bassi. 1996. A CK2 site is reversibly phosphorylated in the photosystem II subunit CP29. FEBS Lett. 399:245-250.
12. Croce, R., J. Breton, and R. Bassi. 1996. Conformational changes induced by phosphorylation in the CP29 subunit of Photosystem II. Biochemistry. 35:11142-11148.
13. Mauro, S., P. Dainese, R. Lannoye, and R. Bassi. 1997. Cold-resistant and cold-sensitive maize lines differ in the phosphorylation of the photosystem II subunit, CP29. Plant Physiol. 115:171-180.
14. Hemminga M. A., and L. J. Berliner, editors. 2007. ESR spectroscopy in membrane biophysics. Springer, New York.
15. Van Oort, B., S. Murali, E. Wientjes, R. B. M. Koehorst, R. B. Spruijt, et al. 2009. Ultrafast resonance energy transfer from a site-specifically attached fluorescent chromophore reveals the folding of the N-terminal domain of CP29. Chem. Phys. 357:113-119.
16. Stopar, D., J. Štrancar, R. B. Spruijt, and M. A. Hemminga. 2005. Exploring the local conformational space of a membrane protein by site-directed spin labeling. J. Chem. Inf. Model. 45:1621-1627.
17. Nagai, K., and H. C. Thøgersen. 1987. Synthesis and sequence-specific proteolysis of hybrid proteins produced in Escherichia coli. Methods Enzymol. 153:461-481.
18. Paulsen, H., U. Rümler, and W. Rüdiger. 1990. Reconstitution of pigment-containing complexes from light-harvesting chlorophyll a/b-binding protein overexpressed inEscherichia coli. Planta. 181:204-211.
19. Porra, R. J., W. A. Thompson, and P. E. Kriedemann. 1989. Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. Biochim. Biophys. Acta. 975:384-394.
20. Davies, B. 1965. Analysis of carotenoid pigments. In Chemistry and Biochemistry of Plant Pigments. T. W. Goodwin, editor. Academy Press, New York. 489-532.
21. Giuffra, E., D. Cugini, R. Croce, and R. Bassi. 1996. Reconstitution and pigment-binding properties of recombinant CP29. Eur. J. Biochem. 238:112-120.
22. Croce, R., M. G. Muller, S. Caffarri, R. Bassi, and A. R. Holzwarth. 2003. Energy transfer pathways in the minor antenna complex CP29 of photosystem II: a femtosecond study of carotenoid to chlorophyll transfer on mutant and WT complexes. Biophys. J. 84:2517-2532.
23. Stopar, D., J. Štrancar, R. B. Spruijt, and M. A. Hemminga. 2006. Motional restrictions of membrane proteins: A site-directed spin labeling study. Biophys. J. 91:3341-3348.
24. Filipič, B., and J. Štrancar. 2001. Tuning EPR spectral parameters with a genetic algorithm. Appl. Soft Comput. 1:83-90.
25. Štrancar, J., T. Koklič, Z. Arsov, B. Filipič, D. Stopar, et al. 2005. Spin label EPR-based characterization of biosystem complexity. J. Chem. Inf. Model. 45:394-406.
26. Kavalenka, A. A., B. Filipič, M. A. Hemminga, and J. Štrancar. 2005. Speeding up a genetic algorithm for EPR-based spin label characterization of biosystem complexity. J. Chem. Inf. Model. 45:1628-1635.
27. Columbus, L., and W. L. Hubbell. 2004. Mapping backbone dynamics in solution with site-directed spin labeling: GCN4-58 bZip free and bound to DNA. Biochemistry. 43:7273-7287.
28. Steinhoff, H. J., A. Savitsky, C. Wegener, M. Pfeiffer, M. Plato, et al. 2000. High-field EPR studies of the structure and conformational changes of site directed spin labeled bacteriorhodopsin. Biochim. Biophys. Acta. 1457:253-262.
29. Štrancar, J., M. Šentjurc, and M. Schara. 2000. Fast and accurate characterization of biological membranes by EPR spectral simulations of nitroxides. J. Magn. Reson. 142:254-265.
30. Štrancar, J., T. Koklič, and Z. Arsov. 2003. Soft picture of lateral heterogeneity in biomembranes. J. Membr. Biol. 196:135-146.
31. Caffarri, S., F. Passarini, R. Bassi, and R. Croce. 2007. A specific binding site for neoxanthin in the monomeric antenna proteins CP26 and CP29 of Photosystem II. FEBS Lett. 581:4704-4710.
32. Paulsen, H., B. Finkenzeller, and N. Kühlein. 1993. Pigments induce folding of light-harvesting chlorophyll a/b-binding protein. Eur. J. Biochem. 215:809-816.
33. Horn, R., G. Grundmann, and H. Paulsen. 2007. Consecutive binding of chlorophylls a and b during the assembly in vitro of light-harvesting chlorophyll-a/b protein (LHCIIb). J. Mol. Biol. 366:1045-1054.
34. Hemelrijk, P. W., S. L. S. Kwa, R. van Grondelle, and J. P. Dekker. 1992. Spectroscopic properties of LHC-II, the main light-harvesting chlorophyll a/b protein complex from chloroplast membranes. Biochim. Biophys. Acta. 1098:159-166.
35. Gradinaru, C. C., I. H. M. van Stokkum, A. A. Pascal, R. van Grondelle, and H. van Amerongen. 2000. Identifying the pathways of energy transfer between carotenoids and chlorophylls in LHCII and CP29. A multicolor, femtosecond pump-probe study. J. Phys. Chem. B. 104:9330-9342.
36. Pascal, A., C. Gradinaru, U. Wacker, E. Peterman, F. Calkoen, et al. 1999. Spectroscopic characterization of the spinach Lhcb4 protein (CP29), a minor light-harvesting complex of photosystem II. Eur. J. Biochem. 262:817-823.
37. Mozzo, M., F. Passarini, R. Bassi, H. van Amerongen, and R. Croce. 2008. Photoprotection in higher plants: The putative quenching site is conserved in all outer light-harvesting complexes of photosystem II. Biochim. Biophys. Acta. 1777:1263-1267.
38. Mozzo, M., L. Dall'Osto, R. Hienerwadel, R. Bassi, and R. Croce. 2008. Photoprotection in the antenna complexes of photosystem II: role of individual xanthophylls in chlorophyll triplet quenching. J. Biol. Chem. 283:6184-6192.
39. Jeschke, G., A. Bender, T. Schweikardt, G. Panek, H. Decker, et al. 2005. Localization of the N-terminal domain in light-harvesting chlorophyll a/b protein by EPR measurements. J. Biol. Chem. 280:18623-18630.