Reverse transcription loop-mediated isothermal amplification for the detection of Porcine reproductive and respiratory syndrome virus

Journal of Veterinary Diagnostic Investigation

Volumn 21, Issue 3, 2009, Pages 350-354

  Rovira, Albert ^a   Abrahante, Juan ^b   Murtaugh, Michael ^b   Muñoz Zanzi, Claudia ^c  

^a UNIVERSITY OF MINNESOTA   (United States)

^b UNIVERSITY OF MINNESOTA   (United States)

^c UNIVERSITY OF MINNESOTA   (United States)

Author keywords

Diagnostic test; Loop mediated isothermal amplification; Polymerase chain reaction; Porcine reproductive and respiratory syndrome virus

Indexed keywords

ANIMALIA; ARTERIVIRUS; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; SUIDAE;

VIRUS DNA; VIRUS RNA;

ANIMAL; ANIMAL DISEASE; ARTERIVIRUS; ARTICLE; CLASSIFICATION; ISOLATION AND PURIFICATION; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; PHOTOMETRY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SWINE;

ANIMALS; DNA, VIRAL; NEPHELOMETRY AND TURBIDIMETRY; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SWINE;

EID: 67650254766     PISSN: 10406387     EISSN: None     Source Type: Journal    
DOI: 10.1177/104063870902100308     Document Type: Article

References (16)

1. Boehme CC, Nabeta P, Henostroza G, et al.: 2007, Operational feasibility of using loop-mediated isothermal amplification for diagnosis of pulmonary tuberculosis in microscopy centers of developing countries. J Clin Microbiol 45:1936-1940.
2. Curtis KA, Rudolph DL, Owen SM: 2008, Rapid detection of HIV-1 by reverse-transcription, loop-mediated isothermal amplification (RT-LAMP). J Virol Methods 151:264-270.
3. Dukes JP, King DP, Alexandersen S: 2006, Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus. Arch Virol 151:1093-1106.
4. Hong TC, Mai QL, Cuong DV: 2004, Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. J Clin Microbiol 42:1956-1961.
5. Indik S, Schmoll F, Sipos W, Klein D: 2005, Genetic variability of PRRS virus in Austria: consequences for molecular diagnostics and viral quantification. Vet Microbiol 107:171-178.
6. Iseki H, Alhassan A, Ohta N, et al.: 2007, Development of a multiplex loop-mediated isothermal amplification (mLAMP) method for the simultaneous detection of bovine Babesia parasites. J Microbiol Methods 71:281-287.
7. Kleiboeker SB, Schommer SK, Lee SM, et al.: 2005, Simultaneous detection of North American and European porcine reproductive and respiratory syndrome virus using real-time quantitative reverse transcriptase-PCR. J Vet Diagn Invest 17:165-170.
8. Larkin MA, Blackshields G, Brown NP, et al.: 2007, ClustalW and ClustalX version 2. Bioinformatics 23:2947-2948.
9. Mori Y, Nagamine K, Tomita N, Notomi T: 2001, Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150-154.
10. Nagamine K, Hase T, Notomi T: 2002, Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes 16:223-229.
11. Notomi T, Okayama H, Masubuchi H, et al.: 2000, Loopmediated isothermal amplification of DNA. Nucleic Acids Res 28:E63.
12. Poon LL, Leung CS, Chan KH, et al.: 2005, Detection of human influenza A viruses by loop-mediated isothermal amplification. J Clin Microbiol 43:427-430.
13. Rovira A, Clement T, Christopher-Hennings J, et al.: 2007, Evaluation of the sensitivity of PCR to detect PRRSV on individual and pooled samples from boars during acute infection. J Vet Diagn Invest 19:502-509.
14. Soliman H, El-Matbouli M: 2006, Reverse transcription loopmediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHS). Vet Microbiol 114:205-213.
15. Wasilk A, Callahan JD, Christopher-Hennings J, et al.: 2004, Detection of U.S., Lelystad, and European-like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR. J Clin Microbiol 42:4453-4461.
16. Zimmerman J, Benfield DA, Murtaugh MP, et al.: 2006, Porcine reproductive and respiratory syndrome virus (Porcine arterivirus). In: Diseases of swine, ed. Straw BE, Zimmerman JJ, D'Allaire S, Taylor DJ, 9th ed., pp. 387-417. Blackwell Publishing, Ames, IA.