Characterizing cell death events using a microfluidics-based method

Biochip Journal

Volumn 2, Issue 4, 2009, Pages 242-247

  Mahto, Sanjeev Kumar ^a   Yoon, Tae Hyun ^b   Rhee, Seog Woo ^a  

^a Kongju National University   (South Korea)

^b Hanyang University   (South Korea)

Author keywords

Apoptosis; Cadmium ion; Cell death; Microfluidic multicompartments; Necrosis

Indexed keywords

EID: 67650230852     PISSN: 19760280     EISSN: 20927843     Source Type: Journal    
DOI: None     Document Type: Article

References (20)

1. Kroemer, G. et al. Classification of cell death: recommendations of the nomenclature committee on cell death. Cell Death Differ. 12, 1463-1467 (2005).
2. Edinger, A.L. & Thompson, C.B. Death by design: apoptosis, necrosis and autophagy. Curr. Opin. Cell Biol. 16, 663-669 (2004).
3. Vaux, D.L. Toward an understanding of the molecular mechanisms of physiological cell death. Proc. Natl. Acad. Sci., U.S.A. 90, 786-789 (1993).
4. Golstein, P. & Kroemer, G. Cell death by necrosis: towards a molecular definition. Trends Biochem. Sci. 32, 38-43 (2006).
5. Krysko, D.V., Berghe, T.V., D'Herde, K. & Vandenabeele, P. Apoptosis and necrosis: Detection, discrimination and phagocytosis. Methods 44, 205-221 (2008).
6. Koopman, G. et al. Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-1420 (1994).
7. Vermes, I., Haanen, C. & Reuteling-Sperger, C. Flow cytometry of apoptotic cell death. J. Immunol. Methods 243, 167-190 (2000).
8. Denecker, G. et al. Phosphatidylserine exposure during apoptosis precedes release of cytochrome c and decrease in mitochondrial transmembrane potential. FEBS Lett. 465, 47-52 (1999).
9. Qin, J., Ye, N., Liu, X. & Lin, B. Microfluidic devices for the analysis of apoptosis. Electrophoresis 26, 3780-3788 (2005).
10. Walker, G.M. et al. Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator. Lab. Chip 5, 611-618 (2005).
11. King, K.R. et al. A high-throughput microfluidic realtime gene expression living cell array. Lab. Chip 7, 77-85 (2007).
12. Thompson, D.M. et al. Dynamic gene expression profiling using a microfabricated living cell array. Anal. Chem. 76, 4098-4103 (2004).
13. Tourovskaia, A., Figueroa-Masot, X. & Folch, A. Differentiation-on-a-chip: A microfluidic platform for long-term cell culture studies. Lab. Chip 5, 14-19 (2005).
14. Wang, Z., Kim, M.C., Marquez, M. & Thorsen, T. High-density microfluidic arrays for cell cytotoxicity analysis. Lab. Chip 7, 740-745 (2007).
15. Ye, N., Qin, J., Liu, X., Shi, W. & Lin, B. Characterizing doxorubicin-induced apoptosis in HepG2 cells using an integrated microfluidic device. Electrophoresis 28, 1146-1153 (2007).
16. Thompson, C.B. Apoptosis in the pathogenesis and treatment of disease. Science 267, 1456-1462 (1995).
17. Fadok, V.A. et al. Different populations of macrophages use either the vitronectin receptor or the phosphatidylserine receptor to recognize and remove apoptotic cells. J. Immunol. 149, 4029-4035 (1992).
18. Taylor, A.M. et al. Microfluidic multicompartment device for neuroscience research. Langmuir 19, 1551-1556 (2003).
19. Whitesides, G.M., Ostuni, E., Takayama, S., Jiang, X. & Ingber, D.E. Soft lithography in biology and biochemistry. Ann. Rev. Biomed. Eng. 3, 335-373 (2001).
20. Holden, M., Kumar, S., Castellana, E., Beskok, A. & Cremer, P. Generating fixed concentration arrays in a microfluidic device. Sens. Actuators B 92, 199-207 (2003).