Chemical modification of recombinant human keratinocyte growth factor 2 with polyethylene glycol improves biostability and reduces animal immunogenicity

Journal of Biotechnology

Volumn 142, Issue 3-4, 2009, Pages 242-249

  Huang, Zhifeng ^a,b   Ni, Chunyan ^a   Chu, Yanhui ^c   Wang, Shanshan ^a   Yang, Shulin ^b   Wu, Xiaoping ^a   Wang, Xiaojie ^a   Li, Xiaokun ^a,b   Feng, Wenke ^a,d   Lin, Shaoqiang ^a  

^a WENZHOU MEDICAL UNIVERSITY   (China)

^b NANJING UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^c MUDANJIANG MEDICAL UNIVERSITY   (China)

^d University of Louisville School of Medicine   (United States)

Author keywords

Chemical modification; Immunogenicity; PEG phenyl isothiocyanate; Recombinant human keratinocyte growth factor 2 (rhKGF 2); Stability

Indexed keywords

BIOSTABILITY; FIBROBLAST GROWTH FACTOR; GEL FILTRATION; IMMUNOGENICITY; ISOTHIOCYANATE; KERATINOCYTE GROWTH FACTORS; MITOGENIC ACTIVITY; MOLECULAR MODIFICATION; N-TERMINAL; PEG-PHENYL-ISOTHIOCYANATE; PEGYLATION; PHYSIOLOGICAL TEMPERATURE; PROTEIN FAMILY; RECOMBINANT HUMAN KERATINOCYTE GROWTH FACTOR 2 (RHKGF-2); SEPHADEX; STRUCTURAL STABILITIES; THERAPEUTIC APPLICATION; THERMAL STABILITY;

AFFINITY CHROMATOGRAPHY; BIOACTIVITY; CELL CULTURE; CHEMICAL MODIFICATION; CHEMICAL STABILITY; CHROMATOGRAPHIC ANALYSIS; ETHYLENE GLYCOL; GEL PERMEATION CHROMATOGRAPHY; GELATION; ION EXCHANGE; PEPTIDES; POLYETHYLENE OXIDES; POLYSACCHARIDES; STABILITY; THERMOPLASTICS;

POLYETHYLENE GLYCOLS;

HEPARIN; MACROGOL; PHENYL ISOTHIOCYANATE; REPIFERMIN; SEPHADEX; SEPHAROSE;

AFFINITY CHROMATOGRAPHY; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CHEMICAL MODIFICATION; CIRCULAR DICHROISM; CONTROLLED STUDY; DRUG BLOOD LEVEL; DRUG HALF LIFE; DRUG PURIFICATION; DRUG STABILITY; DRUG STRUCTURE; EPITHELIUM CELL; FEMALE; GEL FILTRATION; IMMUNOGENICITY; KIDNEY EPITHELIUM; MALE; MITOGENESIS; NONHUMAN; PRIORITY JOURNAL; RAT; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; THERMOSTABILITY;

ANIMALS; CELL LINE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CIRCULAR DICHROISM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; FIBROBLAST GROWTH FACTOR 10; HUMANS; IMMUNOGLOBULIN G; MICE; MICE, INBRED BALB C; MICROSCOPY, FLUORESCENCE; POLYETHYLENE GLYCOLS; PROTEIN DENATURATION; PROTEIN STABILITY; RATS; RECOMBINANT PROTEINS; TRYPSIN; UREA;

ANIMALIA; MUS;

EID: 67650084426     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.05.004     Document Type: Article

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