Evidence that methylation of hepatitis B virus covalently closed circular DNA in liver tissues of patients with chronic hepatitis B modulates HBV replication

Journal of Medical Virology

Volumn 81, Issue 7, 2009, Pages 1177-1183

  Guo, Yanhai ^a   Li, Yongnian ^b   Mu, Shijie ^c   Zhang, Ju ^a   Yan, Zhen ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b Department of Infectious Diseases   (China)

^c FOURTH MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

CpG island; HbeAg; HBV cccDNA; HBV replication; Hepatocyte

Indexed keywords

CIRCULAR DNA; COVALENTLY CLOSED CIRCULAR DNA; HEPATITIS B SURFACE ANTIGEN; HEPATITIS B(E) ANTIGEN; RELAXED CIRCULAR DNA; UNCLASSIFIED DRUG; VIRUS DNA;

ADULT; AGED; ARTICLE; CHRONIC HEPATITIS; CPG ISLAND; DNA METHYLATION; DNA SEQUENCE; FEMALE; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HUMAN TISSUE; MAJOR CLINICAL STUDY; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; SEQUENCE ANALYSIS; VIRION; VIRUS REPLICATION; LIVER; METABOLISM; MIDDLE AGED; PHYSIOLOGY; VIROLOGY;

HEPATITIS B VIRUS;

ADULT; AGED; DNA METHYLATION; DNA, CIRCULAR; DNA, VIRAL; FEMALE; HEPATITIS B VIRUS; HEPATITIS B, CHRONIC; HUMANS; LIVER; MALE; MIDDLE AGED; VIRUS REPLICATION; YOUNG ADULT;

EID: 67649989072     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.21525     Document Type: Article

References (21)

1. Carman WF, Hadziyannis S, Mcgarvey MJ, Jacyna MR, Karayiannis P, Makris A, Thomas HC. 1989. Mutation preventing formation of hepatitis-Be-antigen in patients with chronic hepatitis-B infection. Lancet 2:588-591. (Pubitemid 19216208)
2. Caruntu FA, Molagic V. 2005. CccDNA persistence during natural evolution of chronic VHB infection. Rom J Gastroenterol 14:373-377. (Pubitemid 43027127)
3. Doitsh G, Shaul Y. 2004. Enhancer I predominance in hepatitis B virus gene expression. Mol Cell Biol 24:1799-1808. (Pubitemid 38167100)
4. Hajkova P, el-Maarri O, Engemann S, Oswald J, Olek A, Walter J. 2002. DNA-methylation analysis by the bisulfite-assisted genomic sequencing method. Methods Mol Biol 200:143-154.
5. Jammeh S, Tavner F, Watson R, Thomas HC, Karayiannis P. 2008. Effect of basal core promoter and pre-core mutations on hepatitis B virus replication. J Gen Virol 89:901-909.
6. Koji T, Kondo S, Hishikawa Y, An S, Sato Y. 2008. In situ detection of methylated DNA by histo endonuclease-linked detection of methylated DNA sites: A new principle of analysis of DNA methylation. Histochem Cell Biol 130:917-925.
7. Laras A, Koskinas J, Dimou E, Kostamena A, Hadziyannis SJ. 2006. Intrahepatic levels and replicative activity of covalently closed circular hepatitis B virus DNA in chronically infected patients. Hepatology 44:694-702. (Pubitemid 44433726)
8. Métivier R, Gallais R, Tiffoche C, Le Péron C, Jurkowska RZ, Carmouche RP, Ibberson D, Barath P, Demay F, Reid G, Benes V, Jeltsch A, Gannon F, Salbert G. 2008. Cyclical DNA methylation of a transcriptionally active promoter. Nature 452:45-50. (Pubitemid 351355084)
9. Miller RH, Robinson WS. 1983. Integrated hepatitis B virus DNA sequences specifying the major viral core polypeptide are methylated in PLC/PRF/5 cells. Proc Natl Acad Sci USA 80:2534-2538.
10. Misawa N, Matsumoto A, Tanaka E, Rokuhara A, Yoshizawa K, Umemura T, Maki N, Kinura T, Kiyosawa K. 2006. Patients with and without loss of hepatitisBvirusDNAafter hepatitisBe antigen seroconversion have different virological characteristics. J Med virol 78:68-73. (Pubitemid 41783845)
11. Nicholson WJ, Black SH, Simmonds P, Chung CW, Aw D, Peutherer JF. 1992. Comparison of hepatitis B virus subtyping of d/y determinants by radioimmunoprecipitation assay and the polymerase chain reaction. J Med Virol 36:21-27.
12. Olek A, Oswald J, Walter J. 1996. A modified and improved method for bisulphite based cytosine methylation analysis. Nucleic Acids Res 24:5064-5066. (Pubitemid 27007919)
13. Pollicino T, Squadrito G, Cerenzia G, Cacciola I, Raffa G, Crax A, Farinati F, Missale G, Smedile A, Tiribelli C, Villa E, Raimondo G. 2004. Hepatitis B virus maintains its pro-oncogenic properties in the case of occult HBV infection. Gastroenterology 126:102-110. (Pubitemid 38040728)
14. Pollicino T, Belloni L, Raffa G, Pediconi N, Squadrito G, Raimondo G, Levrero M. 2006. Hepatitis B virus replication is regulated by the acetylation status of hepatitis B virus cccDNA-bound H3 and H4 histones. Gastroenterology 130:823-837.
15. Thomassin H, Oakeley EJ, Grange T. 1999. Identification of 5-methylcytosine in complex genomes. Methods 19:465-475.
16. Thomassin H, Kress C, Grange T. 2004. MethylQuant: A sensitive method for quantifyingmethylation of specific cytosines within the genome. Nucleic Acids Res 32:e168.
17. Vivekanandan P, Thomas D, Torbenson M. 2008a. Hepatitis B viral DNA is methylated in liver tissues. J Viral Hepat 15:103-107.
18. Vivekanandan P, Kannangai R, Ray SC, Thomas DL, Torbenson M. 2008b. Comprehensive genetic and epigenetic analysis of occult hepatitis B from liver tissue samples. Clin Infect Dis 46:1227-1236.
19. Wieland SF, Spangenberg HC, Thimme R, Purcell RH, Chisari FV. 2004. Expansion and contraction of the hepatitis B virus transcriptional template in infected chimpanzees. Proc Natl Acad Sci USA 101:2129-2134.
20. Wu TT, Coates L, Aldrich CE, Summers J, Mason WS. 1990. In hepatocytes infected with duck hepatitis B virus, the template for viral RNA synthesis is amplified by an intracellular pathway. Virology 175:255-261.
21. Zoulim F. 2005. New insight on hepatitis B virus persistence from the study of intrahepatic viral cccDNA. J Hepatol 42:302-308.