High-titer preparation of Bombyx mori nucleopolyhedrovirus (BmNPV) displaying recombinant protein in silkworm larvae by size exclusion chromatography and its characterization

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Kato, Tatsuya ^a   Manoha, Suganthi Lavender ^a   Tanaka, Shigeyasu ^a   Park, Enoch Y ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS; BINDING ANALYSIS; BOMBYX MORI NUCLEOPOLYHEDROVIRUS; CORRELATION COEFFICIENT; END POINTS; ENVELOPE PROTEINS; HUMAN (PRO)RENIN RECEPTOR; HUMAN PRORENIN; LARGE-SCALE PRODUCTION; NUCLEOCAPSID PROTEINS; PCR METHOD; PEPTIDE SEQUENCES; PROTEIN PATTERNS; QUANTITATIVE REAL TIME PCR; RAPID METHOD; RECOMBINANT BACULOVIRUS; RECOMBINANT PROTEIN; SILKWORM LARVAE; TRANS-MEMBRANE PROTEINS;

AMINES; BLOOD; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; NUCLEIC ACIDS; PROTEINS; PURIFICATION;

SIZE EXCLUSION CHROMATOGRAPHY;

NUCLEOCAPSID PROTEIN; PRORENIN; VIRUS ENVELOPE PROTEIN; CELL SURFACE RECEPTOR; PRORENIN RECEPTOR; RECOMBINANT PROTEIN;

ARTICLE; CONTROLLED STUDY; GEL PERMEATION CHROMATOGRAPHY; HEMOLYMPH; NONHUMAN; POLYHEDROSIS VIRUS; PROTEIN ANALYSIS; PROTEIN BINDING; REAL TIME POLYMERASE CHAIN REACTION; SILKWORM; VIRUS CHARACTERIZATION; VIRUS PURIFICATION; VIRUS REPLICATION; VIRUS TITRATION; ANIMAL; BIOSYNTHESIS; GEL CHROMATOGRAPHY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LARVA; METABOLISM; ULTRACENTRIFUGATION; VIROLOGY; VIRUS CULTURE;

BOMBYX MORI; BOMBYX MORI NPV;

ANIMALS; BOMBYX; CHROMATOGRAPHY, GEL; HEMOLYMPH; HUMANS; LARVA; NUCLEOPOLYHEDROVIRUS; RECEPTORS, CELL SURFACE; RECOMBINANT PROTEINS; ULTRACENTRIFUGATION; VIRUS CULTIVATION;

EID: 67649708608     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-55     Document Type: Article

References (34)

1. Sanghani PC Moran RC Purification and characterization of recombinant human folylpoly-γ-glutamate synthase expressed at high levels in insect cells Protein Expr Purif 2000, 18:36-45. 10.1006/prep.1999.1173 10648167
2. Maranga L Rueda P Antonis AGF Vela C Langeveld JPM Casal JI Carrondo MJT Large scale production and downstream processing of a recombinant porcine parvovirus vaccine Appl Microbiol Biotechnol 2002, 59:45-50. 10.1007/s00253-002-0976-x 12073130
3. Fipaldini C Bellei B La Monica N Expression of hepatitis C virus cDNA in human hepatoma cell line mediated by a hybrid baculovirus-HCV Vector Virology 1999, 255:320-311. 10.1006/viro.1998.9565
4. Tani H Limn CK Yap CC Onishi M Nozaki M Nishimune Y Okahashi N Kitagawa Y Watanabe R Mochizuki R Moriishi K Matsura Y In vitro and in vivo gene delivery by recombinant baculoviruses J Virol 2003, 77:9799-9808. 224587 12941888 10.1128/JVI.77.18.9799-9808.2003
5. Hu Y Baculovirus as a highly efficient expression vector in insect and mammalian cells Acta Pharmacol Sin 2005, 26:405-416. 10.1111/ j.1745-7254.2005.00078.x 15780188
6. Loisel TP Ansanay H St-Onge S Gay B Boulanger P Strosberg AD Marullo S Bouvier M Recovery of homogeneous and functional β 2-adrenergic receptors from extracellular baculovirus particles Nat Biotechnol 1997, 15:1300-1304. 10.1038/nbt1197-1300 9359116
7. Masuda K Itoh H Sakihama T Akiyama C Takahashi K Fukuda R Yokomizo T Shimizu T Kodama T Hamakubo T A combinatorial G protein-coupled receptor reconstitution system on budded baculovirus: Evidence for Gαi and Gαo coupling to a human leukotriene B4 receptor J Biol Chem 2003, 278:24552-24562. 10.1074/jbc.M302801200 12721292
8. Kost TA Condreay JP Recombinant baculoviruses as mammalian cell gene-delivery vectors Trends Biotechnol 2002 20 173 180 10.1016/ S0167-7799(01)01911-4 11906750
9. Wang Y Wu X Liu G Cao C Huang H Xu Z Liu J Expression of porcine lactoferrin by using recombinant baculovirus in silkworm, Bombyx mori L., and its purification and characterization Appl Microbiol Biotechnol 2005, 69:385-389. 10.1007/s00253-005-1998-y 15856222
10. Yue W Miao Y Li X Wu X Zhao A Nagasaki M Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-bac/BmNPV baculovirus expression system Appl Microbiol Biotechnol 2006, 73:181-186. 10.1007/s00253-006-0462-y 16804693
11. Du D Kato T Nabi AH Suzuki F Park EY Expression of functional human (pro)renin receptor in silkworm (larvae) using BmNPV bacmid Biotechnol Appl Biochem 2008, 49:195-202. 10.1042/BA20070136 17705788
12. Pastori L Concentration of recombinant baculovirus by cation-exchange chromatography BioTechniques 1999, 26:834-840. 10337471
13. Transfiguracion J Jorio H Meghrous J Jacob D Kamen A High yield purification of functional baculovirus vectors by size exclusion chromatography J Virol Methods 2007, 142:21-28. 10.1016/ j.jviromet.2007.01.002 17306891
14. Wu C Soh KY Wang S Ion-exchange membrane chromatography method for rapid and efficient purification of recombinant baculovirus and baculovirus gp64 protein Hum Gene Ther 2007, 18:665-672. 10.1089/hum.2007.020 17604565
15. Kato T Park EY Specific expression of GFPuv-β 1,3-N-acetylglucosaminyltransferase 2 fusion protein in fat body of Bombyx mori silkworm larvae using signal peptide Biochem Biophys Res Commun 2007, 359:543-548. 10.1016/j.bbrc.2007.05.137 17544364
16. Hink WF Vail PV A plaque assay for titration of alfalfa looper nuclear polyhedrosis virus in a cabbage looper TN-368 cell line J Invertebr Pathol 1973, 22:168-174. 10.1016/0022-2011(73)90129-8
17. Cha HJ Gotoh T Bentley WE Simplification of titer determination for recombinant baculovirus by Green fluorescent protein marker BioTechniques 1997, 23:782-786. 9383535
18. Kwon MS Dojima T Toriyama M Park EY Development of an antibody-based assay for determination of baculovirus titers in 10 hours Biotechnol Prog 2002, 18:647-651. 10.1021/bp020298s 12052086
19. Lo H Rapid titer determination of baculovirus by quantitative real-time polymerase chain reaction Biotechnol Prog 2004, 20:354-360. 10.1021/ bp034132i 14763863
20. Lee KS Kim BY Je YH Woo SD Sohn HD Jin BR A new technique for producing recombinant baculovirus directly in silkworm larvae Biotechnol Lett 2007, 29:175-180. 10.1007/s10529-006-9215-3 17091380
21. Braunagel SC Summers MD Autographa californica nuclear polyhedrosis virus, PDV, and ECV viral envelopes and nucleocapsids: Structural proteins, antigens, and lipid and fatty acid profiles Virology 1994, 202:315-328. 10.1006/viro.1994.1348 8009843
22. Gerald CJ Arboleda MJ Solar G Mule JJ Kerr WG A rapid and quantitative assay to estimate gene transfer into retrovirally transduced hematopoietic stem/progenitor cells using a 96-well format PCR and fluorescent detection system universal for MMLV-based proviruses Hum Gene Ther 1996, 7:343-354. 10.1089/hum.1996.7.3-343 8835221
23. Sanburn N Cornetta K Rapid titer determination using quantitative real-time PCR Gene Ther 1999, 6:1340-1345. 10.1038/sj.gt.3300948 10455446
24. Ma L Bluyssen HA De Raeymaeker M Laurysens V Beek van der N Pavliska H van Zonneveld AJ Tomme P van Es HH Rapid determination of adenoviral vector titers by quantitative real-time PCR J Viol Methods 2001, 93:181-188. 10.1016/S0166-0934(01)00257-9
25. Wang R Wang J Li J Wang Y Xie Z An L Comparison of two gel filtration chromatographic methods fot the purification of Lily symptomless virus J Virol Methods 2007, 139:125-131. 10.1016/j.jviromet.2006.09.008 17207540
26. Loa CC Lin TL Wu CC Bryan TA Thacker HL Hooper T Schrader D Purification of turkey coronavirus by Sephacryl size-exclusion chromatography J Virol Methods 2002, 104:184-194. 10.1016/S0166-0934(02)00069-1
27. Zhang Y Lv Z Chen J Chen Q Quan Y Kong L Zhang H Li S Zheng Q Chen J Nie Z Wang J Jin Y Wu X A novel method for isolation of membrane proteins: A baculovirus surface display system Proteomics 2008, 8:4178-4185. 10.1002/ pmic.200800133 18814327
28. Jin R Lv Z Chen Q Quan Y Zhang H Li S Chen G Zheng Q Jin L Wu X Chen J Zhang Y Safty and immunogenicity of H5N1 influenza vaccine based on baculovirus surface display system of Bombyx mori PLoS ONE 2008, 3:e3933. 2592543 19079592 10.1371/journal.pone.0003933
29. van Lier FLJ Homberg van den JPTW de Gooijer CD den Boer MM Vlak JM Tramper J Long-term semi-continuous production of recombinant baculovirus protein in a repeated (fed-)batch two-stage reactor system Enzyme Microb Technol 1996, 18:460-466. 10.1016/0141-0229(95)00129-8
30. Krell PJ Passage effect of virus infection in insect cells Cytotechology 1996, 20:125-137. 10.1007/BF00350393
31. Kool M Voncken JW van Lier FLJ Tramper J Vlak JM Detection and analysis of Autographa californica nuclear polyhedrosis virus mutants with defective interfering properties Virology 1991, 183:739-746. 10.1016/ 0042-6822(91)91003-Y 1853572
32. Yanase T Hashimoto Y Matsumoto T Analysis of defective genomes of Bombyx mori nucleopolyhedrovirus generated by serial undiluted passage in cell culture Acta Virol 1998, 42:65-70. 9770071.
33. Kato T Kageshima A Suzuki F Park EY Expression and purification of human (pro)renin receptor in insect cells using baculovirus expression system Protein Expr Purif 2008, 58:242-248. 10.1016/j.pep.2007.11.011 18171623
34. Motohashi T Shimojima T Fukagawa T Maenaka K Park EY Efficient large-scale protein production of larvae and pupae of silkworm by Bombyx mori nuclear polyhedrosis virus bacmid system Biochem Biophys Res Commun 2004, 326:564-569. 10.1016/j.bbrc.2004.11.060