A rapid and quantitative assay to estimate gene transfer into retrovirally transduced hematopoietic stem/progenitor cells using a 96-well format PCR and fluorescent detection system universal for MMLV-based proviruses

Human Gene Therapy

Volumn 7, Issue 3, 1996, Pages 343-354

  Gerard, Christopher J ^a   Arboleda, Mary Jo ^a   Solar, Gregg ^a   Mulé, James J ^b   Kerr, William G ^a,c  

^a NOVARTIS PHARMA AG   (Switzerland)

^b UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^c HOSPITAL OF THE UNIVERSITY OF PENNSYLVANIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; ETHIDIUM BROMIDE; EXONUCLEASE;

ARTICLE; CELL CULTURE; CONTROLLED STUDY; EXPRESSION VECTOR; FLUORESCENCE ANALYSIS; GENE TRANSFER; GENETIC TRANSDUCTION; HEMATOPOIETIC STEM CELL; HUMAN; HUMAN CELL; MOLONEY LEUKEMIA ONCOVIRUS; POLYMERASE CHAIN REACTION; PROVIRUS; RETROVIRUS;

ACUTE DISEASE; BASE SEQUENCE; CELL CULTURE TECHNIQUES; CLONE CELLS; DNA PRIMERS; DNA, RECOMBINANT; DNA, VIRAL; FLUOROMETRY; GENETIC VECTORS; HEMATOPOIETIC STEM CELLS; HUMANS; LEUKEMIA, MYELOID; MOLECULAR SEQUENCE DATA; MOLONEY MURINE LEUKEMIA VIRUS; POLYMERASE CHAIN REACTION; PROVIRUSES; SENSITIVITY AND SPECIFICITY; TRANSFECTION; TUMOR CELLS, CULTURED;

MOLONEY MURINE LEUKEMIA VIRUS; MURINAE; ONCOVIRINAE; UNIDENTIFIED RETROVIRUS;

EID: 0030024721     PISSN: 10430342     EISSN: None     Source Type: Journal    
DOI: 10.1089/hum.1996.7.3-343     Document Type: Article

References (22)

1. ARMENTANO, D., YU, S-F., KANTOFF, P.W., VON RUDEN, T., ANDERSON, W.F., and GILBOA, E. (1987). Effect of internal viral sequences on the utility of retroviral vectors. J. Virol. 16, 1647-1650.
2. BENDER, M.A., PALMER, T.D., GELINAS, R.E., and MILLER, D.A. (1987). Evidence that the packaging signal of Moloney Murine Leukemia virus extends into the gag region. J. Virol. 61, 1639-1646.
3. BONNEROT, C., ROCANCOURT, D., BRIAND, P., GRIMBER, G., and NICOLAS, J-F. (1987). A β-galactosidase hybrid protein targeted to nuclei as a marker for developmental studies. Proc. Natl. Acad. Sci. USA 84, 6795-6799.
4. BRANDT, J., BAIRD, N., LU, L., SROUR, E.F., and HOFFMAN, R. (1988). Characterization of a human hematopoietic progenitor cell capable of forming blast colonies in vitro. J. Clin. Invest. 82, 1017.
5. BREGNI, M., MAGNI, M., SIENA, S., DI NICOLA, M., BONADONNA, G., and GIANNI, A.M. (1992). Human peripheral blood hematopoietic progenitors are optimal targets of retroviral-mediated gene transfer. Blood 80, 1418-1422.
6. CASSEL, A., COTTLER-FOX, M., DOREN, S., and DUNBAR, C.E. (1993). Retroviral-mediated gene transfer into CD34-enriched human peripheral blood stem cells. Exp. Hematol. 21, 585-591.
7. DICK, J.E., KAMEL-REID, S., MURDOCH, B., and DOEDEN, S.M. (1991). Gene transfer into normal human hematopoietic cells using in vitro and in vivo assays. Blood 78, 624-634.
8. HOLLAND, P.M., ABRAMSON, R.D., WATSON, R., and GELFAND, D.H. (1991). Detection of specific polymerase reaction product by utilizing the 5′ → 3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88, 7276-7280.
9. HWU, P., SHAFER, G.E., TREISMAN, J., SCHINDLER, D.G., GROSS, G., COWHERD, R., ROSENBERG, S.A., and ESHAR, Z. (1993). Lysis of ovarian cancer cells by human lymphocytes redirected with a chimeric gene composed of an antibody variable region and the Fc receptor γ chain. J. Exp. Med. 178, 361-366.
10. JOLLY, D. (1994). Viral vector systems for gene therapy. Cancer Gene Ther. 1, 51-64.
11. KOEFFLER, H.P., BILLING, R., LUSIS, A.J., SPARKES, R., and GOLDE, D.W. (1980). An undifferentiated variant derived from the human acute myelogenous leukemia cell line (KG-1). Blood 56, 265-273.
12. LEE, G.L., CONNELL, C.R., and BLOCH, W. (1993). Allelic discrimination by nick-translation PCR with fluorogenic probes. Nucleic Acids Res. 21, 3761-3766.
13. + hematopoietic stem/progenitor cells from human umbilical cord blood. J. Exp. Med. 178, 2089-2096.
14. + cells purified from peripheral blood of breast cancer patients primed with chemotherapy and granulocyte-macrophage colony-stimulating factor. Hum. Gene Ther. 5, 203-208.
15. MILLER, D.A., and ROSMAN, G.J. (1989). Improved retroviral vectors for gene transfer and expression. BioTechniques 7, 980-990.
16. MORGAN, R.A., CORNETTA, K., and ANDERSON, W.F. (1990) Applications of the polymerase chain reaction in retroviral-mediated gene transfer and the analysis of gene-marked human TIL cells. Hum. Gene Ther. 1, 135-149.
17. NOLAN, G.P., FIERING, S., NICOLAS, J-F., and HERZENBERG, L.A. (1988). Fluorescence-activated cell analysis and sorting of viable mammalian cells based on β-D-galactosidase activity after transduction of Escherichia coli lacZ. Proc. Natl. Acad. Sci. USA 85, 2603-2607.
18. RAMASWAMY, N., TARE, N.S., BENJAMIN, W.R., and GUBLER, U. (1989). A sensitive technique to monitor gene transfer and expression in bone marrow stem cells. Exp. Hematol. 17, 832-835.
19. RILL, D.R., BUSCHLE, M., FOREMAN, M.K., BARTHOLOMEW, C., MOEN, R.C., SANTANA, V.M., IHLE, J.M., and BRENNER, M.K. (1992). Retrovirus-mediated gene transfer as an approach to analyze neuroblastoma relapse after autologous bone marrow transplantation. Hum. Gene Ther. 3, 129-136.
20. RYCHLIK, W. (1993). Selection of primers for polymerase chain reaction. In Methods in Molecular Biology, Vol. 15: PCR Protocols, Current Methods and Applications. B.A. White, ed. (Humana Press, Inc., Totowa, NJ) pp. 31-40.
21. SAIKI, R.K. (1989). Design and optimization of the PCR. In PCR Technology: Principles and Applications for DNA Amplification. H.A. Erlich, ed. (Stockton Press, New York) pp. 7-16.
22. SAMBROOK, J., FRITSCH, E.F., and MANIATIS, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed. (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY).