Construction of the plasmid, expression by Chinese hamster ovary cell, purification and characterization of the first three short consensus repeat modules of human complement receptor type 1

Journal of Biochemistry

Volumn 145, Issue 4, 2009, Pages 533-542

  Yamaguchi, Atsushi ^a,b   Takagawa, Hiroaki ^a,b   Iwakaji, Hirofumi ^b   Miyagawa, Shuji ^c   Wang, Pi Chao ^a,b   Ishii, Noriyuki ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c OSAKA UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

CHO cell; Complement control activity; Complement receptor type 1 (CR1); Short consensus repeat (SCR1 3); Sugar chain

Indexed keywords

ASPARAGINE LINKED OLIGOSACCHARIDE; COMPLEMENT COMPONENT C3B RECEPTOR; DNA; GLYCOSIDASE; PROTEIN; RESTRICTION ENDONUCLEASE; COMPLEMENT RECEPTOR; MERCAPTOETHANOL;

ANIMAL CELL; ARTICLE; CHINESE HAMSTER; CHO CELL; COMPLEMENT SYSTEM; CONSENSUS; DNA SEQUENCE; FEMALE; GENE EXPRESSION SYSTEM; GENE OVEREXPRESSION; HEMOLYSIS; INFLAMMATION; NONHUMAN; OVARY CELL; PLASMID; PROTEIN EXPRESSION; PURIFICATION; STROMA CELL; WESTERN BLOTTING; AMINO ACID SEQUENCE; ANIMAL; CELL FRACTIONATION; CHEMISTRY; CONSENSUS SEQUENCE; CRICETULUS; DRUG EFFECT; GENETICS; GLYCOSYLATION; HAMSTER; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CRICETULUS GRISEUS;

ANIMALS; BLOTTING, WESTERN; CHO CELLS; CONSENSUS SEQUENCE; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOSYLATION; HUMANS; MERCAPTOETHANOL; PLASMIDS; RECEPTORS, COMPLEMENT; REPETITIVE SEQUENCES, AMINO ACID; SUBCELLULAR FRACTIONS;

EID: 67649670260     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvp006     Document Type: Article

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