Combination of in vivo cryptorchid testis and in vitro co-culture system to obtain high purification and proliferation of mouse spermatogonial stem cells

International Journal of Fertility and Sterility

Volumn 2, Issue 3, 2008, Pages 115-120

  Absalan, Forouzan ^a   Movahedin, Mansoureh ^a   Mowla, Seyed Javad ^a  

^a TARBIAT MODARES UNIVERSITY   (Iran)

Author keywords

Co culture; Colonization; Cryptorchidism; Spermatogonial

Indexed keywords

OCTAMER TRANSCRIPTION FACTOR 4; VIMENTIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL CULTURE; CELL ISOLATION; CELL PROLIFERATION; CELL SURVIVAL; COCULTURE; CRYPTORCHISM; CULTURE MEDIUM; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; IN VIVO STUDY; MALE; MOUSE; NONHUMAN; SERTOLI CELL; SPERMATOGONIUM; STEM CELL; TESTIS;

EID: 67649522056     PISSN: 2008076X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Shinohara T, Mary R, Avarbock, Brinster RF. Functional analysis of spermatogonial stem cells in steel and cryotorchid infertile mouse model. Dev Biol. 200; 220: 401-411.
2. Bellve AR, Cavicchia JC, Milletteelection Cf, O'Brien DA, Bhatnagar YM, Dym M. Spermatogenic cells of the prepubertal mouse. Isolation and morphological characterization. J Cell Biol. 1977; 74: 68-85.
3. Morena AR, Boitani C, Pesca M, De felici M, Stefanini M. Isolation of highly purified type A spermatogonia from prepubertal rat testis. J Androl. 1996; 17: 708-717.
4. Dirami G, Ravindranath N, Purcel V, Dym M. Effects of stem cell factor and granulocyte macrophage-colony stimulating factor on survival of porcine type A spermatogonia cultured in KSOM. Biol Reprod. 1999; 61: 225-230.
5. Izadyar F, Ouden KD, Creemers LB, Posthuma G, Parvinen M, de Rooij DG. Proliferation and Differentiation of Bovine Type A spermatogonia During Long-Term Culture. Biol Reprod. 2003; 68: 272-81.
6. Shinohara T, Orwig KE, Avarbock MR, Brinster RL. Remodeling of the post natal mouse testis in accompanied by dramatic changes in stem cell number and niche accessibility. Proc Natl Acad Sci USA. 2001; 98: 6186-6191.
7. kanatsu-Shinohara M, Ogonuki N, Inoue K, Ogura A, Toyokuni S, Shinohara T. Restoration of fertility in fertile mice by transplantation of cryopreserved male germline stem cells. Hum Reprod. 2003; 18: 2660-67.
8. Kubota H, Avarbock MR, Brinster RL. Growth factors essential for self-renewal and expression of mouse spermatogonial stem cells. Proc Natl Accad Sci USA. 2004; 101: 16489-94.
9. Tadokoro Y, Yomoida K, Ohta H, Tohda A, Nishimune Y. Homeostatic regulation of germinal stem cell proliferation by the GDNF/FSH pathway. Mech Dev. 2002; 113: 29-39.
10. Kern S, Robertson SA, Mau VJ, Maddocks S. Cytokine secretion by macrophages in the rat testis. Biol Reprod. 1995; 53: 1407-16.
11. van Pelt AMM, Morena AR, van Dissel-Emiliani FMF, Boitani C, Gaemers IC, de Rooij DG, et al. Isolation of the synchoronized A spermatogonia from adult vitamin A-deficient rat testes. Biol Reprod. 1996; 55: 439-444.
12. Pesce M, Scholer HR. Oct-4: control of totipotency and germ line determination. Mol Reprod Dev. 2000; 55: 452-457.
13. Scarpino S, Morena AR, Petersen C, Froysa B, Soder O, Boitani CA. 1998 Rapid method of Sertoli cells isolation by DSA lectin, allowing mitotic analyses. Mol Cell Endocrinol. 1998; 146: 121-27.
14. Anway MD, Folmer J, Wright WW, Zirk BR. Isolation of Sertoli cells from Adult Rat Testes: An Approach to Ex Vivo Studies of Sertoli cells Function. Biol Reprod. 2003; 68: 996-1002.
15. Jeong D, McLean DJ, Griswold MD. Long-term culture and transplantation of murine testicular germ cells. J Androl. 2003; 24: 661-69.
16. Shi Y Q, Wang QZ, Liao SY, Zhang Y, Liu YX, Han CS. In vitro propagation of spermatogonial stem cells from KM mice. Front Biosci. 2006; 11: 2614-22.
17. Bucci LR, Brock WR, Johnson TS, Meistrich ML. Isolation and biochemical studies of enriched populations of spermatogonia and early primary spermatocytes from rat testis. Biol Reprod. 1986; 34: 195-206.
18. Nishimune Y, Aizawa S, Komatsu T. Testicular germ cell differentiation in vivo. Fertil Steril. 1978; 29: 95-102.
19. Shinohara H, Avarbock MR, Brinster RL. β1-and a6- integrines are surface markers on mouse spermatogonial stem cells. Proc Natl Acad Sci USA. 1999; 96: 5504-5509.
20. Harrison DE, Lerner CP. Most primitive hematopoietic stem cells are stimulated to cycle rapidly after treatment with 5-flurouracil. Blood. 1991; 78: 1237-1240.
21. Nagano M, Avarbock MR, Leonida EB, Brinster JC, Brinster RL. Culture of mouse spermatogonial stem cells. Tissue Cell. 1998; 30: 389-97.
22. Mori C, Nakamura N, Dix DJ, Fujioka M, Nakagawa S, Shiota K. Morphological analysis of germ cell apoptosis during postnatal testis development in normal and Hsp 70-2 knockout mice. Dev Dyn. 1997; 208:125-136.
23. Oke BO Suarez-Quian CA. Localization of secretory, membrane-associated and cytoskeletal proteins in rat testis using an improved immunocytochemical protocol that employs polyester wax. Biol Reprod. 1993; 48: 621-31.