Overview on HLA and DNA typing methods

Biotecnologia Aplicada

Volumn 22, Issue 2, 2005, Pages 91-101

  Ferrer, Annia ^a   Fernández, María E ^a   Nazabal, Marcelo ^a  

^a CENTER FOR GENETIC ENGINEERING AND BIOTECHNOLOGY   (Cuba)

Author keywords

DNA typing; Genetic of HLA; HLA antigens; HLA nomenclature; Human MHC; Polymerasa chain reaction (PCR); Sequence based typing (SBT); Sequence specific oligo nucleotide (SSO) probes; Sequence specific primers (SSP)

Indexed keywords

EID: 67649394060     PISSN: 08644551     EISSN: 10272852     Source Type: Journal    
DOI: None     Document Type: Review

References (111)

1. Hugh H, Fudenberg JRL, An-Chuan Wang, Ferrara GB. Basic immunogenetics. 3rd ed., London: Oxford UniversityPress, 1984.
2. Terasaki PI. History of HLA: Ten Recollections. ed., Los Angeles: UCLA Tissue Typing Laboratory Press, 1990.
3. Glenn E. HLA beyond tears. Introduction to Human Histocompatibility. Second edition. Atlanta: De Novo Inc, 1991.
4. Sanfilippo F, Amos DB. An interpretation of the major histocompatibility complex. In: Rose NR, Friedman H, Fahey JL, editors. Manual of clinical laboratory immunology, 3rd ed. Washington: ASM Press, 1986.
5. De Jong MM, Nolte IM, de Vries EG, Schaapveld M, Kleibeuker JH, Oosterom E, Oosterwijk JC, van der Hout AH, van der Steege G, Bruinenberg M, Boezen HM, Te Meerman GJ, van der Graaf WT. The HLA class III subregion is responsible for an increased breast cancer risk. Hum Mol Genet 2003;12(18):2311-9.
6. Roitt M, Brostoff J, Male DK. T-cell receptors and major histocompatibility complex molecules. In: Immunology. 5th ed. London: Churchill Livingston, 1998.
7. Lotteau V. Assembly and intracellular transport of HLA molecule antigen presenters. Ann Rech Vet 1992;23(3):268-74.
8. Browning M, Me Michael A. HLA and MHC: genes, molecules and function. London: Bios Scientific Publishers.1996.
9. Marsh SG, Albert ED, Bodmer WF, et al. HLA Nomenclature Report. Tissue Antigens 2002;60:407-64.
10. Tiercy JM, Marsh SG, Schreuder GM, Albert E, Fischer G, Wassmuth R. Guidelines for nomenclature usage in HLA reports: ambiguities and conversion to serotypes. Eur J Immunogenet 2002;29(3):273-4.
11. Tiercy JM, Marsh SG, Schreuder GM, Albert E, Fischer G, Wassmuth R. EFI Guidelines for Nomenclature usage in HLA Reports. European Journal of Immunogenetics 2002;29:273-4.
12. Sullivan KA, Amos DB. The HLA system and its detection. In: Rose NR, FriedmilO H, Fahey JL, editors. Manual of clinical laboratory immunology, 3rd ed Washington: ASM Press, 1986.
13. Jin P, Wang E. Polymorphism in clinical immunology|From HLA typing to immunogenetic profling. J Transl Med 2003; 1:1-11.
14. Piancatelli D. Human leukocyte antigen-A, -B, and -Cw polymorphism in a Berber population from North Morocco using sequence-based typing. Tissue Antigens 2004. 26:78-87.
15. Clayton J, Lonjou C. Allele and haplotype frequencies for HLA loci in various ethnic groups. In: Charron D, editor. Genetic diversity of HLA, functional and medical implications. Paris: EDK Publishers, 1997;1:665-820.
16. Shankarkumar U, Pednekar SV, Gupte S, Ghosh K, Mohanty D. HLA antigen distribution in Marathi speaking hindu population from Mumbai, Maharashtra, India. J Hum1999;105:367-72.
17. Mehra NK, Taneja V, Kailash S, Raizada N, Vaidya MC. Distribution of HLA antigens in a sample of North Indian. Hindu population. Tissue Antigens 1986; 27:64-74.
18. Pitchappan RM, Kakkanaiah VN, Rajasekar R, Arulraj N, Muthukkaruppan VR. HLA antigens in South India: I. Major groups of Tamil Nadu. Tissue Antigens 1984;24:190-6.
19. Thomas DG, Francis SC, David G, editors. Principles of medical genetics. Baltimore: Williams and Wilkins, 1998.
20. Shankarkumar U. Studies on the immunogenetic basis of selected diseases in South India. Ph. D. thesis. Madurai: Madurai Kamaraj University.1994.
21. Dupont B, Ed.: Immunobiology of HLA. New York: Springer-Verlag. 1989.
22. Bignon D, Semana G, Tiercy M, Simons M, Lalouellm, Cohen D. DNA-RFLP analysis: HLA-DR beta workshop report. In: Dupont B, ed. Immunobiology of HLA. New York: Springer-Verlag, 1989:851-60.
23. Barker D, Schafer M, White R. Restriction sites containing CpG show a higher frequency of polymorphism in human DNA. Cell 1984:36:131-8.
24. Angelini G, Prev Al Cd, Gorski J, Mach B. High-resolution analysis of the human HLA-DR polymorphism by hybridization with sequencespecific oligonucleotide probes. Proc Natl Acad Sci USA 1986;83: 4489-93.
25. Gorskij, et al. HLA-DRa, DXa and DR-lll gene association studies in DR3 individuals. Hum Immunol 1987;20: 273-8.
26. Amar A, Holbeck Sl, Nepom G. Specific allelic variation among linked HLA class II genes. Transplantation 1987;44:831-5.
27. Le Gall I, et al. Two DR- allelic series defined by exon II specific synthetic oligonucleotide genomic hybridization: a method of HLA typing Proc Natl Acad Sci (USA) 1986;83:7836-40.
28. Tiercy JM, Gorski J, Betuel H, Freidel AC, Gebuhrer L, Jeannet M, Mach B. DNA typing of DRw6 subtypes: correlation with DRB1 and DRB3 alleleic sequences by hybridization with oligonucleotide probes. Hum Immunol1989;24:1-14.
29. Ucla C, Van Rood J, Gorski J, Mach B. Analysis of HLA-D micropolymorphism by a simple procedure: RNA oligonucleotide hybridization. J Clin Invest 1987;80: 1155-9.
30. Freeman SM, Noreen HJ, Bach FH. Oligonucleotide probing. Applications to HLA typing. Arch Pathol Lab Med 1988;112:22-7.
31. Bidwellll, et al. HLA-DR and -DQ ty-ping by DNA-RFLP analysis. In: Hill KM, Bidwell lL, eds. Handbook of HLA typing techniques. London: CRC Press 1993; 71-98.
32. Gyllensten VB, Erlich HA. Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus. Proc Natl Acad Sci (USA) 1988;85:7652-6.
33. Ennis Pd, Zemmourj, Salter Rd, Parham P. Rapid cloning of HLA-A, B cDNA by using the polymerase chain reaction: frequency and nature of errors produced in amplification. Proc Natl Acad Sci (USA) 1990;87:2833-7.
34. Entz P, Toliat MR, Hampe J, Valentonyte R, Jenisch S, Nurnberg P, Nagy M. New strategies for efficient typing of HLA class-II loci DQB1 and DRB1 by using Pyrose-quencing. Tissue Antigens 2005;65(1): 67-80.
35. Saiki RK, Buga Wan TL, Horn GT, Mullis KB, Erlich HA. Analysis of enzymatically amplified-globin and HLA- DQa DNA with allele-specific oligonucleotide probes. Nature 1986;324:163-6.
36. Saiki R, Chang CA, Levenson CH, Boehm CD, Kazazian RH, Erlich HA. Rapid genetic analysis of enzymatically-amplified DNA with non-radioactive allele-specific oligo-nucleotide probes. Am J Hum Genet 1987;41:A237.
37. Bugawan TL, Horn GT, Long CM, Mickelson E, Hansen JA, Ferrara GB, Angelini G, Erlich HA. Analysis of HLA-DP alleleic sequence polymorphism using the in vitro enzymatic DNA amplification of DPa and DP-loci. J Immunol1988;141:4024-30.
38. Horn GT, Buga Wan TL, Long CM, Erlich HA. HLA class II antigens and susceptibility to insulin-dependent diabetes mellitus. Proc Natl Acad Sci (USA) 1988;85: 6012-16.
39. Morel C, Arlen F, Jeannet M, Mach B, Tiercy JM. Complete analysis of HLA-DQB1 polymorphism and DR-DQ linkage disequilibrium by oligonucleotide typing. Hum Immunol 1990;29:64-77.
40. Erlich HA, Scharf SJ, Long CM, Horn GT. Analysis of isotypic and allotypic sequence variation in the HLA-DRb region using the in vitro enzymatic amplification of specific DNA segments. In: DUPONT B, ed. Immunobiology of HLA. New York: Springer-Verlag 1989;181-7.
41. Baxter-Lowe LA, Hunter B, Casper JT, Gorski J. HLA gene amplification and hybridization analysis of polymorphism. J Clin Invest 1989;84:613-8.
42. Nevinny-Stickel C, Bettinotti MD, Andreas A, Hinzpeter M, Muhlegger K, Schmitz G, Albert ED. Non-radioactive HLA class II typing using polymerase chain reaction and digoxigenen-11-2′-3′dideoxyuridinetriphosphate- labelled oligonucleotide probes. Hum Immunol 1991;31:7-13.
43. Shaffer AL, Falk-Wade JA, Tortorelli V, Cigan A, Carter C, Hassan K, Hurley CK. HLA-DRw52-associated DRBI alleles: identification using polymerase chain reaction-amplified DNA, sequence-specific oligonucleotide probes, and a chemiluminescent detection system. Tissue Antigens 1992;39:84-90.
44. Gao X, Fernandez-Vina M, Shumway W, Stastny P. DNA typing for class II HLA antigens with allele-specific or group specific amplification. 1. Typing for subsets of HLADR4. Hum Immunol 1990;27:40-50.
45. Fugger L, Morling N, Ryder Lp, Odum N, Sve Gaard A. Technical aspects of typing for HLA-DP alleles using allele-specific DNA in vitro amplification and sequence-specific oligonucleotide probes. Detection of single base mismatches. J Immunol Methods 1990;129:175-85.
46. Cailla TS, Garchon HJ, Bach F. Automation of large-scale HLA Class II oligotyping using a robotic work station. In: Tsuki K, Alzawa M, Sasasuki T, eds. HLA 1991. Oxford: Oxford University Press, 1992:346-7.
47. Oh SH, Fleischhauer K, Yang SY. Isoelectric focusing subtypes of HLA-A can be defined by oligonucleotide typing. Tissue Antigens 1993;41(3):135-42.
48. Shankarkumar U, Ghosh K, Badakere H, Mohanty D. Novel HLA Class I Alleles Associated with Indian Leprosy Patients. Journal of Biomedicine and Biotechnology 2003;3:208-11.
49. Ng J, Hurley CK, Carter C, Baxter-Lowe LA, Bing D, Chopek M,, et al. Large-scale DRB and DQB1 oligonucleotide typing for the NMDP registry: progress report from year 2. Tissue Antigens 1996;47:21-6.
50. Noreen H, Steiner L, Davidson M, Johnson S, Segall M, Begovich Ab. Six new DPB1 alleles identified in a study of 1,302 unrelated bone marrow donor-recip-ient pairs. Tissue Antigens 1997;49:5126.
51. Buga W An TL, Begovich AB, Erlich HA. Rapid HLA-DPB typing using enzymatically amplified DNA and non-radioactive sequence-specific oligonucleotide probes. Immunogenetics 1990;32:231-41.
52. Buyse I, Decorte R, Baens M, Cuppens H, Semana G, Emonds MP, et al. Rapid DNA typing of class II HLA antigens using the polymerase chain reaction and reverse dot blot hybridization. Tissue Antigens 1993;41:114.
53. Cros P, Allibert P, Mandrand B, Tiercey LM, Mach B. Oligonucleotide genotyping of HLA polymorphism on microtitre plates. Lancet 1992;340:8703.
54. Lazaro AM, Fernandezvina MA, Lru Z, Stastny P. Enzyme-linked DNA oligotyping. A practical method for clinical HLA-DNA typing. Hum Immunol 1993;36:243-8.
55. Nevinny-Stickel C, Albert Ed. HLA class II typing in a microtitre plate formate using digoxigenin-labelled amplified DNA and biotin-labelled oligonucleotide probes. Eur J Immunogenet 1993;20:419-27.
56. Matsubara K, Koide Y, Kobayashi A, Kaida R, Takeda S, Matsuda E, et al. A rapid and sensitive method for HLA-DRB1 typing by acridinium-ester-labelled DNA probes. Hum Immunol1992;35:132-9.
57. Etokebe GE, Opsahl M, Tveter AK, Lie BA, Thorsby E, Vartdal F, Spurkland A. Physical separation of HLA-A alleles by denaturing high-performance liquid chromatography. Tissue Antigens 2003; 61:443-50.
58. Cox ST, McWhinnie AJ, Robinson J, Marsh SG, Parham P, Madrigal JA, Little AM. Cloning and sequencing full-length HLAB and C genes. Tissue Antigens 2003; 61(1):20-48.
59. Williams F, Mallon E, Middleton D. Development of PCR-SSOP for HLA-A typing of bone marrow registry donors. Tissue Antigens 1997;49:61-6.
60. Shankarkumar U, Prasanavar D, Ghosh K, Mohanty D, et al. HLA A*02 allele frequencies and B haplotype associations in Western Indians. Hum. Immunol. 2003;64:552-556.
61. Fernandez-Vina M, Lazaro AM, Sun Y, Miller S, Forero L, Stastny P. Population diversity of B-locus alleles observed by high-resolution DNA typing. Tissue Antigens 1995;45:153-68.
62. Parham P, Adams EJ, Arnett KL. The origins of HLA-A, B, C polymorphism. Immunol Rev 1995;143:141-80.
63. Ishikawa Y, Tokunaga K, Tanaka H, Nishimura M, Muraoka M, Fujii Y, et al. HLA-A null allele with a stop codon, HLAA* 0215N, identified in a homozygous state in a healthy adult. Immunogenetics 1995;43:1-5.
64. Magor KE, Taylor EJ, Shen SY, Martinez-Naves E, Valiante NM, Wells RS, et al. Natural inactivation of a common HLA allele (A *2402) has occurred on at least three separate occasions. J Immunol, 1997;158: 5242-50.
65. Arguello R, Avakian H, Goldman LM, Madrigal A. A novel method for simultaneous high resolution identification of HLA-A, HLA-B and HLA-Cw alleles. Proc Natl Acad Sci (USA) 1996;93:10961-5.
66. Fischer GF, F Ae I, Petrasek M, Moser S. A combination of two distinct in vitro amplification procedures for DNA typing of HLA-DRB and -DQB1 alleles. Vox Sang 1995;69:328-35.
67. Fischer GF, Fae I, Moser S, Petrasek M, Bassler HM, Menzel GR, Mayr WR, et al. Ligation based HLAB*27 typing. Tissue Antigens 1996;48:148-52.
68. Uryu N, Maeda M, Ata M, Tsuji K, Inoko H. A simple and rapid method for HLA-DRB and -DQB typing by digestion of PER-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 1990;35:20-31.
69. Maeda M, Murayama N, Ishii H, Uryu N, Ota M, Tsuji K, Inoko H. A simple and rapid method for HLA-DQA1 genotyping by digestion of PCR-amplified DNA with allele specific restriction endonucleases. Tissue Antigens 1989;34:290-8.
70. Maeda M, Uryu N, Murayama N, Ishii H, Ota M, Tsuji K, Inoko H. A simple and rapid method for HLA-DP genotyping by digestion of peR-amplified DNA with allele specific restriction endonucleases. Hum Immunol, 1990;27:111-21.
71. Alerup 0. HLA class II typing by digestion of PER-amplified DNA with allele-restriction endonucleases will fail to unequivocally identify the genotypes of many homozygous and heterozygous individuals. Tissue Antigens 1990;36:83-7.
72. Yunis I, Salazar M, Yunis EJ. HLA-DR generic typing by RFLP. Tissue Antigens 1991;38:78-88.
73. Ata M, Seki T, Fukushima H, Tsuji K, Inoko H. HLA-DRB1 genotyping by modified PCR-RFLP method combined with group-specific primers. Tissue Antigens 1992;39:187-202.
74. Nomura N, Ata M, Tsuji K, Lnoko H. HLA-DQBl genotyping by a modified RFLP method combined with group specific primers. Tissue Antigens 1991;38:53-9.
75. Ata M, et al. Modified PCR-RFLP method for HLA-DPB1 and -DQA1 genotyping. Tissue Antigens 1991;38:60-71.
76. Witzke SA, Witzke SA, Wardrh. HLA-DPB typing using co-digestion of amplified fragments allows efficient identification of heterozygous genotypes. Tissue Antigens 1992;40:175-81.
77. Nomura N, Ata M, Kato S, Inoko H, Tsuji K. Severe acute graft-versus-host disease by HLA-DPBl disparity in recombinant family of bone marrow transplantation between serologically HLA-identical siblings: an application of the polymerase chain reaction-restriction fragment length polymorphism method. Hum Immunol 1991;32:261-8.
78. Nieto A, Tobes R, Martin J, Pareja E. Allele walking: a new and highly accurate approach to HLA-DRB1 typing. Application to DRBl *04 alleles. Tissue Antigens 1997;49:141-51.
79. Santamaria P, Boyce-Acino M T, Lindstrom AL, Barbosa JJ, F Aras AJ, Rich SS. HLA class II "typing": direct sequencing of DRB, DQB and DQA genes. Hum Immunol 1992;33:69-81.
80. Spurkland A, Knutsen I, Markussen G, V Artdal F, Egeland T, Thorsby E. HLA matching of unrelated bone marrow transplant pairs: direct sequencing of in vitro amplified HLA-DRB1 and -DQB1 using magnetic beads as solid support. Tissue Antigens 1993;41:155-64.
81. Knipper AJ, Hinney A, Schuch B, Enczmann J, Uhrberg M, Wemet P. Selection of unrelated bone marrow donors by PCR-SSP typing and subsequent non-radioactive sequencebased typing for HLA DRBl/3/4/5, DQBl and DPBl alleles. Tissue Antigens 1994;44:275-84.
82. Santamaria P, Lindstrom AL, Boyce-Jacino MT, Myster SH, Barbosa JJ, Faras AJ, Rich SS. HLA class I sequence-based typing. Hum Immunol 1993;37:39-50.
83. Mcginnis MD, Conrad MP, Bouwens AG, Tilanus MG, Kronick MN. Automated, solid-phase sequencing of DRB region genes using T7 sequencing chemistry and dye-labeled primers. Tissue Antigens 1995;46:173-9.
84. Duquesnoy RJ. HLA Matchmaker: a molecularly based algorithm for histocompatibility determination. Description of the algorithm. Hum Immunol 2002;63:339-52.
85. Wang L, Marincola FM. Applaying pyrosequencing to HLA-typing. ASHI Quarterly 2003;27:16-8.
86. Sharon D, Kathleen C, Barracchini, Chen D, Robbins F, Stroncek D, Francesco M. Marincola Ambiguous Allele Combinations in Sequence-based Typing. ASHI Quarterly Fourth Quarter 2003.
87. Petersdorf EW, Stanley F, Martin PJ, Hansen JA. Molecular diversity of the HLA-C locus in unrelated marrow transplantation. Tissue Antigens 1994;44:939.
88. Petersdorf EW, Setoda T, Smith AG, Hansen JA. Analysis of HLA-B*44 alleles encoded on extended HLA haplotypes by direct automated sequencing. Tissue Antigens 1994;44:211-6.
89. Alerup A, Zetterquist H. HLA-DR ty-ping by PCR amplification with sequence-specific primers (PCR SSP) in 2 hours: an alternative to serological DR typing in clinical practice including donor-recipient matching in cadaveric transplantation. Tissue Antigens 1992;39:225-35.
90. Alerup A, Zetterquist H. DR "low-resolution" PCR-SSP typing a correction and an up-date. Tissue Antigens 1993;41:55-6.
91. Newton CR, Graham A, Heptinstall LE. Analysis of any point mutation in DNA. The amplification refractory system (ARMS). Nucleic Acids Res 1989;17:2503-16.
92. Park MS, Tonal R. Phenotype frequencies of the class II (DR, DQ) DNA alleles by the patterns of sequence-specific primer mixtures (SSPM) in four different populations and the probable haplotypes between DRB1 allele and DQBl allele. In: Terasaki PI, Cecka M, eds. Clinical transplants 1992. Los Angeles: UCLA Tissue Typing Laboratory, 1992;475-500.
93. Bein G, Glaser R, Kirchner H. Rapid HLA-DRB1 genotyping by nested PCR amplification. Tissue Antigens 1992;39:68-73.
94. Zetterquist H, Bengtsson M, Backstrom G, Egle-Jansson I, Ekdahl AM, Grunnet N, et al. Report from the HLA class II typing by PCR-SSP multicentre study. Eur J Immunogenet 1997;24:191-9.
95. Mytilineos J, Lempert M, Middleton D, Williams F, Scherer S, Apelz G. HLA class I DNA typing of 215 "HLA-A, -B, DR zero mismatched" kidney transplants. Tissue Antigens 1997;50:355-8.
96. Savelkoul PHM, De Bruyn-Geraets DP, Van Den Berg-Loonen EM. High resolution HLA-DRB1 SSP typing for cadaveric donor transplantation. Tissue Antigens 1995;45:41-8.
97. Fass SJ, Menon R, Braun ER, Rudert WA, Trucco M. Sequence-specific priming and exonuclease-released fluorescence detection of HLA-DQB1 alleles. Tissue Antigens 1996; 48:97-112.
98. Alms Camps M, Ludeck H, Sla Tev AK, Blascyk R. The taqman fluorotyping technique-a new way for differential automated detection of amplified products in the HLA-A, Band C PCR-SSP. Eur J Immunogenet 1997; 24:126.
99. Bidwell L, Hui KM. Human HLA-DR/Dw allotype matching by analysis of HLA-DRB gene PCR product polymorphism (PCR "fingerpints"). Technique 1990;2:939.
100. Clay TM, Bidwell L, Howard MR, Bradley BA. PCR-fringerprinting for selection of HLA matched unrelated marrow donors. Lancet 1991;337:104952.
101. Bidwell, JL, Clay, TM, Wood, NAP, Pursall, MC, Martin, AF, Bradley, BA and Hui, KM. Rapid HLA-DR-Dw and DP matching by PCR finger-printing and related DNA heteroduplex technologies. In: HUI KM, BIDWELL]L, eds. Handbook of HLA typing techniques. London: CRC Press, 1993;99-115.
102. Uhrberg M, Hinney A, Enczmann J, Wernet P. Analysis of the HLA-DR gene locus by temperature gradient gel electrophoresis and its application for the rapid selection of unrelated bone manow donors. Electrophoresis 1994;15:104450.
103. Madrigal, J.A., Argüello, R., Scott, I. and Avakian, H. High resolution HLA Class I and II typing for unrelated bone marrow donors. Eur J Immunogenet 1997;24:70.
104. Eberle M, Knapp LA, Iw Anaga KK, Domanicom J, Aiyer K, Watkinsdl HLA-B ty-ping by allele separation followed by direct sequencing. Tissue Antigens 1997;49:365-75.
105. Knapp L A, Lehmann E, Hennes L, Eberle ME, Watkinsdl. High resolution HLA-DRB typing using denaturing gradient gel electrophoresis and direct sequencing. Tissue Antigens 1997;50:170-7.
106. Carrington M, Miller T, White M, Gerrard B, Stewart C, Dean M, Mann D. Typing of HLADQA1 and DQB1 using DNA singlestrand conformation polymorphism. Hum Immunol 1992;33:208-12.
107. Summers C, Fergusson W, Gokhale D, Taylorm. Donor-recipient bone marrow matching by single strand conformation polymorphism analysis. Lancet 1992; 339:621.
108. Orita M, Iwahana H, Kanazawa H, Hayashi K, Sekiya T. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc Natl Acad Sri USA 1989;86:2766-70.
109. Kimura A, Hoshino S, Yoshida M, Harada H, Sasazukl T. Polymerase chain reaction-single strand conformation polymorphism (pCR-SSCP) analysis of HLA genes: a practical HLA-matching system. In: Tsuji K, Aizawa M, Sasazuki T, eds. HLA 1991. Oxford: Oxford University Press 1992;511-4.
110. Hayashi T, Seyama T, Ito T, Kusunoki Y, Hirai Y, Nakamura N, Akiyama M.. A simple and rapid method for HLA-DQA1 genotyping by polymerase chain reaction-single strand conformation polymorphism and restriction enzyme cleavage analysis. Electrophoresis 1992;13:877-9.
111. Lo Y, Patel P, Mehal WZ, Fleming KA, Bell JI, W Ainscoa T Js. Analysis of complex genetic systems by ARMS-SSCP: application to HLA genotyping. Nucleic Acids Res 1992; 20:1005-9.