Aspirin acetylates nitric oxide synthase type 3 in platelets thereby increasing its activity

Cardiovascular Research

Volumn 83, Issue 1, 2009, Pages 123-130

  O'Kane, Peter ^a   Xie, Liping ^b   Liu, Zhen ^b   Queen, Lindsay ^c   Jackson, Graham ^a   Ji, Yong ^b   Ferro, Albert ^c  

^a GUY'S AND ST THOMAS' NHS FOUNDATION TRUST   (United Kingdom)

^b NANJING MEDICAL UNIVERSITY   (China)

^c KING'S COLLEGE LONDON   (United Kingdom)

Author keywords

Acetylation; Aspirin; Nitric oxide; Nitric oxide synthase; Phosphorylation

Indexed keywords

ACETYLSALICYLIC ACID; ARGININE; CALCIUM ION; CITRULLINE; CYCLIC GMP; ENDOTHELIAL NITRIC OXIDE SYNTHASE; FURA 2; NITRIC OXIDE;

ACETYLATION; ARTICLE; CONTROLLED STUDY; DRUG MECHANISM; ENZYME ACTIVITY; ENZYME MODIFICATION; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; TANDEM MASS SPECTROMETRY; THROMBOCYTE; WESTERN BLOTTING;

ACETYLATION; ADOLESCENT; ADULT; ANTI-INFLAMMATORY AGENTS, NON-STEROIDAL; ASPIRIN; BLOOD PLATELETS; CYCLIC GMP; DOSE-RESPONSE RELATIONSHIP, DRUG; ENZYME ACTIVATION; HELA CELLS; HUMANS; MALE; NITRIC OXIDE; NITRIC OXIDE SYNTHASE TYPE III; SERINE; TRANSFECTION; YOUNG ADULT;

EID: 67649327409     PISSN: 00086363     EISSN: 17553245     Source Type: Journal    
DOI: 10.1093/cvr/cvp120     Document Type: Article

References (29)

1. Steering Committee of the Physicians' Health Study Research Group. Final report on the aspirin component of the ongoing Physicians' Health study. N Engl J Med 1989;321:129-135.
2. Antiplatelet Trialists' Collaboration. Collaborative overview of randomized trials of antiplatelet therapy. I: Prevention of death, myocardial infarction, and stroke by prolonged antiplatelet therapy in various categories of patients. BMJ 1994;308:81-106.
3. Antithrombotic Trialists' Collaboration. Collaborative meta-analysis of randomized trials of antiplatelet therapy for prevention of death, myocardial infarction, and stroke in high risk patients. BMJ 2002;324:71-86.
4. Yusuf S, Zhao F, Mehta SR, Chrolavicius S, Tognoni G, Fox KK. Clopidogrel in Unstable Angina to Prevent Recurrent Events Trial Investigators. Effects of clopidogrel in addition to aspirin in patients with acute coronary syndromes without ST segment elevation. N Engl J Med 2001;345:494-502.
5. ISIS-2 (Second International Study of Infarct Survival) Collaborative Group. Randomised trial of intravenous streptokinase, oral aspirin, both, or neither among 17,187 cases of suspected acute myocardial infarction ISIS-2. Lancet 1988;13:349-360.
6. Zarraga IG, Schwarz ER. Coxibs and heart disease: what we have learned and what else we need to know. J Am Coll Cardiol 2007;49:1-14.
7. Krotz F, Hellwig N, Schiele TM, Klauss V, Sohn HY. Prothrombotic potential of NSAID in ischemic heart disease. Mini Rev Med Chem 2006;6:1351-1355.
8. Hawkins D, Pinckard RN, Farr RS. Acetylation of human serum albumin by acetylsalicylic acid. Science 1968;160:780-781.
9. Pinckard RN, Hawkins D, Farr RS. In vitro acetylation of plasma proteins, enzymes and DNA by aspirin. Nature 1968;219:68-69.
10. 2- Adrenoceptors activate nitric oxide synthase in human platelets. Circ Res 2000;87:39-44.
11. O'Kane PD, Queen LR, Ji Y, Reebye V, Stratton P, Jackson G et al. Aspirin modifies nitric oxide synthase activity in platelets: effects of acute versus chronic aspirin treatment. Cardiovasc Res 2003;59:152-159.
12. Madajka M, Korda M, White J, Malinski T. Effect of aspirin on constitutive nitric oxide synthase and the biovailability of NO. Thromb Res 2003;110:317-321.
13. Karmohapatra SK, Chakraborty K, Kahn NN, Sinha AK. The role of nitric oxide in aspirin induced thrombolysis in vitro and the purification of aspirin activated nitric oxide synthase from human blood platelets. Am J Hematol 2007;82:986-995.
14. Chakraborty K, Khan GA, Banerjee P, Ray U, Sinha AK. Inhibition of human blood platelet aggregation and the stimulation of nitric oxide synthesis by aspirin. Platelets 2003;14:421-427.
15. Fleming I, Busse R. Molecular mechanisms involved in the regulation of the endothelial nitric oxide synthase. Am J Physiol Regul Integr Comp Physiol 2003;284:R1-R12.
16. Gkaliagkousi E, Ritter J, Ferro A. Platelet-derived nitric oxide signaling and regulation. Circ Res 2007;101:654-662.
17. Chen ZP, Mitchelhill KI, Michell BJ, Stapleton D, Rodriguez-Crespo I, Witters LA et al. AMP-activated protein kinase phosphorylation of endothelial NO synthase. FEBS Lett 1999;43:285-289.
18. Fulton D, Gratton JP, McCabe TJ, Fontana J, Fujio Y, Walsh K et al. Regulation of endothelium-derived nitric oxide production by the protein kinase Akt. Nature 1999;399:597-601.
19. Bauer PM, Fulton D, Boo YC, Sorescu GP, Kemp BE, Jo H et al. Compensatory phosphorylation and protein-protein interactions revealed by loss of function and gain of function mutants of multiple serine phosphorylation sites in endothelial nitric-oxide synthase. J Biol Chem 2003;278:14841-14849.
20. Michell BJ, Harris MB, Chen ZP, Ju H, Venema VJ, Blackstone MA et al. Identification of regulatory sites of phosphorylation of the bovine endothelial nitric-oxide synthase at serine 617 and serine 635. J Biol Chem 2002;277:42344-42351.
21. Matsubara M, Hayashi N, Jing T, Titani K. Regulation of endothelial nitric oxide synthase by protein kinase C. J Biochem (Tokyo) 2003;133:773-781.
22. Alheid U, Frolich JC, Forstermann U. Endothelium-derived relaxing factor from cultured human endothelial cells inhibits aggregation of human platelets. Thromb Res 1987;47:561-571.
23. de Graaf JC, Banga JD, Moncada S, Palmer RMJ, de Groot PG, Sixma JJ. Nitric oxide functions as an inhibitor of platelet adhesion under flow conditions. Circulation 1992;85:2284-2290.
24. Broekman MJ, Eiroa AM, Marcus AJ. Inhibition of human platelet reactivity by endothelium-derived relaxing factor from human umbilical vein endothelial cells in suspension: blockade of aggregation and secretion by an aspirin-insensitive mechanism. Blood 1991;78:1033-1040.
25. Freedman JE, Loscalzo J, Barnard MR, Alpert C, Keaney JF, Michelson AD. Nitric oxide released from activated platelets inhibits platelet recruitment. J Clin Invest 1997;100:350-356.
26. Freedman JE, Ting B, Hankin B, Loscalzo J, Keaney JFJ, Vita JA. Impaired platelet production of nitric oxide in patients with unstable angina. Circulation 1998;98:1481-1486.
27. Queen LR, Ji Y, Goubareva I, Ferro A. Nitric oxide generation mediated by β-adrenoceptors is impaired in platelets from patients with type 2 diabetes mellitus. Diabetologia 2003;46:1474-1482.
28. Pedersen AK, FitzGerald GA. Dose-related kinetics of aspirin. Presystemic acetylation of platelet cyclooxygenase. N Engl J Med 1984;311:1206-1211.
29. Nia B, Vergnaud JM. Comparative pharmacokinetics of Aspegic 1000 mg i.v. versus 1000 mg i.m. thrice daily. Eur J Drug Metab Pharmacokinet 1996;21:333-338.