Evaluation of hydroxyurea-induced fetal skeletal changes in Dutch Belted rabbits by micro-computed tomography and alizarin red staining

Birth Defects Research Part B - Developmental and Reproductive Toxicology

Volumn 86, Issue 3, 2009, Pages 220-226

  Wise, L David ^a   Winkelmann, Christopher T ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Alizarin red staining; Developmental toxicity; Fetal rabbit skeleton; HU; Hydroxyurea; Micro computed tomography; Micro ct; Skeletal evaluation

Indexed keywords

ALIZARIN; ALIZARIN RED; HYDROXYUREA; POLYSTYRENE; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ARTICLE; BONE STRUCTURE; CESAREAN SECTION; CONTROLLED STUDY; EXPERIMENTAL RABBIT; FEMALE; FETUS MALFORMATION; IMAGE RECONSTRUCTION; MICRO-COMPUTED TOMOGRAPHY; NONHUMAN; OSSIFICATION; PRIORITY JOURNAL; SKELETON MALFORMATION;

ANIMALS; ANTHRAQUINONES; BONE AND BONES; BONE DISEASES, DEVELOPMENTAL; CESAREAN SECTION; COLORING AGENTS; FEMALE; HYDROXYUREA; MOTHERS; PREGNANCY; RABBITS; STAINING AND LABELING; TERATOGENS; X-RAY MICROTOMOGRAPHY;

ORYCTOLAGUS CUNICULUS;

EID: 67649280536     PISSN: 15429733     EISSN: None     Source Type: Journal    
DOI: 10.1002/bdrb.20198     Document Type: Article

References (8)

1. Bab I, Hajbi-Yonissi C, Gabet Y, Müller R. 2007. Micro-tomographic atlas of the mouse skeleton. New York: Springer Science+Business Media, LLC.
2. DeSesso JM, Jordan RL. 1977. Drug-induced limb dysplasias in fetal rabbits. Teratology 15:199-212.
3. Guldberg RE, Lin ASP, Coleman R, Robertson G, Duvall C. 2004. Microcomputed tomography imaging of skeletal development and growth. Birth Defects Res C 72:250-259.
4. Oest M, Jones JC, Hatfield C, Prater MR. 2008. Micro-CT evaluation of murine fetal skeletal development yields greater morphometric precision over traditional clear-staining methods. Birth Defects Res B 83:582-589.
5. Redfern BG, Wise LD. 2007. High-throughput staining for the evaluation of fetal skeletal development in rabbits and rabbits. Birth Defects Res 80:177-182.
6. Ritman EL. 2004. Micro-computed tomography-current status and developments. Ann Rev Biomed Eng 6:185-208.
7. Szabo KT, Free SM, Kirkhead HA, Kang YJ, Alston E, Henry M. 1971. The embryotoxic and teratogenic effects of various agents in the fetal mouse and rabbit. Tox Appl Pharm 19:371-372.
8. Winkelman CT, Wise LD. 2009. High-throughput micro-computed tomography imaging as a method to evaluate rat and rabbit fetal skeletal abnormalities for developmental toxicity studies. J Pharm Tox Methods 59:156-165.