Validation of two real-time RT-PCR methods for foot-and-mouth disease diagnosis: RNA-extraction, matrix effect, uncertainty of measurement and precision

Journal of Virological Methods

Volumn 160, Issue 1-2, 2009, Pages 157-162

  Goris, Nesya ^a   Vandenbussche, Frank ^a   Herr, Cécile ^a   Villers, Jérôme ^a   Van der Stede, Yves ^a   De Clercq, Kris ^a  

^a VETERINARY AND AGROCHEMICAL RESEARCH CENTRE   (Belgium)

Author keywords

Diagnosis; Foot and mouth disease; Matrix effect; Real time RT PCR; Uncertainty of measurement; Validation

Indexed keywords

VIRUS RNA;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; DILUTION; EPITHELIUM; FECES ANALYSIS; FOOT AND MOUTH DISEASE; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SPLEEN; TONGUE; UNCERTAINTY; VALIDATION STUDY;

ANIMALS; BLOOD; DIAGNOSTIC ERRORS; FOOT; FOOT-AND-MOUTH DISEASE; FOOT-AND-MOUTH DISEASE VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

FOOT-AND-MOUTH DISEASE VIRUS;

EID: 67549120772     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.05.005     Document Type: Article

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