A fast and robust method to clone and functionally validate T-cell receptors

Journal of Immunological Methods

Volumn 346, Issue 1-2, 2009, Pages 45-54

  Birkholz, Katrin ^a   Hofmann, Christian ^a   Hoyer, Stefanie ^a   Schulz, Birgit ^a   Harrer, Thomas ^a   Kämpgen, Eckhart ^a   Schuler, Gerold ^a   Dörrie, Jan ^a   Schaft, Niels ^a  

^a UNIVERSITY HOSPITAL ERLANGEN   (Germany)

Author keywords

Cellular immunology; Immunotherapy; Signal transduction; T cells; TCR

Indexed keywords

CYTOKINE; HLA A2 ANTIGEN; MELAN A; MESSENGER RNA; RNA; T LYMPHOCYTE RECEPTOR; T LYMPHOCYTE RECEPTOR ALPHA CHAIN; T LYMPHOCYTE RECEPTOR BETA CHAIN; TRANSCRIPTION FACTOR NFAT;

5' UNTRANSLATED REGION; ARTICLE; CELL ACTIVATION; CELL CLONE; CELL PROLIFERATION; CELL STIMULATION; CONTROLLED STUDY; CYTOKINE RELEASE; DNA MODIFICATION; ELECTROPORATION; GENE AMPLIFICATION; HUMAN; HUMAN CELL; IMMUNOTHERAPY; LEUKEMIA CELL LINE; MOLECULAR CLONING; MOLECULAR RECOGNITION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; REPORTER GENE; SEQUENCE ANALYSIS;

ANTIGEN PRESENTATION; ANTIGENS, NEOPLASM; CELL PROLIFERATION; CLONING, MOLECULAR; CYTOKINES; ELECTROPORATION; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; GENES, REPORTER; HLA-A2 ANTIGEN; HUMANS; JURKAT CELLS; LYMPHOCYTE ACTIVATION; NEOPLASM PROTEINS; NFATC TRANSCRIPTION FACTORS; PROMOTER REGIONS, GENETIC; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SIGNAL TRANSDUCTION; T-LYMPHOCYTES; TRANSFECTION;

EID: 67449161547     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.05.001     Document Type: Article

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