Improving IC50 Results with Acoustic Droplet Ejection

JALA - Journal of the Association for Laboratory Automation

Volumn 11, Issue 4, 2006, Pages 240-246

  Olechno, Joseph ^a   Shieh, Jean ^a   Ellson, Richard ^a  

^a Labcyte Inc   (United States)

Author keywords

acoustic droplet ejection; ADE; aqueous intermediate dilutions; DMSO impact on assays; dose response; Echo; IC50

Indexed keywords

BIOASSAY; CONCENTRATION (PROCESS); DRUG DOSAGE; HYDROPHOBICITY; PRECIPITATION (CHEMICAL); SOLUTIONS; SOLVENTS;

ACOUSTIC DROPLET EJECTION; AQUEOUS INTERMEDIATE DILUTIONS; DIMETHYL SULFOXIDE (DMSO);

PHARMACODYNAMICS;

CHEMICAL COMPOUND; DIMETHYL SULFOXIDE; NANOPARTICLE; ORGANIC SOLVENT; PLASTIC;

ACOUSTIC DROPLET EJECTION; ANALYTICAL ERROR; AQUEOUS SOLUTION; ARTICLE; ASSAY; CONCENTRATION (PARAMETERS); COST BENEFIT ANALYSIS; DILUTION; EXPERIMENTAL DESIGN; FALSE NEGATIVE RESULT; FLUID TRANSPORT; HYDROPHOBICITY; IC 50; PIPETTE; PRECIPITATION; SAMPLE SIZE; SOLUBILITY;

EID: 33746904414     PISSN: 15355535     EISSN: 15402452     Source Type: Journal    
DOI: 10.1016/j.jala.2006.06.007     Document Type: Article

References (10)

1. Spicer, T.; Fitzgerald, Y.; Burford, N.; Matson, S.; Chatterjee, M.; Gilchrist, M.; Myslik, J.; O'Connell, J. Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.
2. Ellson R., and Papen R. Sound advice: acoustic technologies facilitate ultra-low volume liquid handling. Today Chem. Work October (2003) 35-38
3. Hampton, S.; Ashworth, D.; Robson, P.; Sawyer, D.; Elliott, L.; Barnett, J.; Roe, M.; Yea, C.; Haigh, R. Library maintenance for the smaller pharma. Poster at the 11th Anniversary Meeting of the Society for Biomolecular Screening, Geneva, Switzerland, September 2005.
4. Cesarek, J.; Nie, D. New tool for automating serial dilutions for activity confirmation experiments. Poster at LabAutomation 2005, January 2005, San Jose, CA.
5. Ellson R., Mutz M., Browning B., Lee L., Miller M.F., and Papen R. Transfer of low nanoliter volumes between microplates using focused acoustics-automation considerations. JALA 8 5 (2003) 29-34
6. Harris D., and Mutz M. Debunking the myth: validation of fluorescein for testing the precision of nanoliter dispensing. JALA 11 4 (2006) 233-239
7. Tjernberg, A.; Markova, N.; Hallén, D. Is DMSO a friend - or a troublemaker? Poster presented at the 11th Anniversary Meeting of the Society for Biomolecular Screening, Geneva, Switzerland, September 2005.
8. Tjernberg A., Markova N., Griffiths W.J., and Hallén D. DMSO-related effects in protein characterization. J. Biomol. Screen 11 2 (2006) 131-137
9. Walsh L., Hastwell P.W., Keenan P.O., Knight A.W., Billinton N., and Walmsey R.M. Genetic modification and variations in solvent increase the sensitivity of the yeast RAD54-GFP genotoxicity assay. Mutagenesis 20 5 (2005) 317-327
10. Masse, F.; Guiral, S.; Kargman, S.; Brideau, C. Compound transfer to 3456-well assay plates using the Echo 550. Poster presented at the 11th Anniversary Meeting of the Society for Biomolecular Screening, Geneva, Switzerland, September 2005.