Ebolavirus VP35 interacts with the cytoplasmic dynein light chain 8

Journal of Virology

Volumn 83, Issue 13, 2009, Pages 6952-6956

  Kubota, Toru ^a,b   Matsuoka, Mayumi ^a,b   Chang, Tsung Hsien ^b   Bray, Mike ^c   Jones, Steven ^d   Tashiro, Masato ^a   Kato, Atsushi ^a   Ozato, Keiko ^b  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

^c NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^d PUBLIC HEALTH AGENCY OF CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

DYNEIN ADENOSINE TRIPHOSPHATASE; UNCLASSIFIED DRUG; VIRUS PROTEIN; VIRUS RNA; VP35 PROTEIN; CORE PROTEIN; DYNLL1 PROTEIN, HUMAN; NUCLEOPROTEIN; NUCLEOPROTEIN VP35, EBOLA VIRUS;

ANIMAL CELL; ARTICLE; BINDING SITE; CONTROLLED STUDY; CYTOPLASM; EBOLA VIRUS; HUMAN; HUMAN CELL; LIFE CYCLE; NONHUMAN; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN PROTEIN INTERACTION; RNA VIRUS; VIRUS GENOME; VIRUS REPLICATION; VIRUS VIRULENCE; CELL LINE; CHEMISTRY; METABOLISM; PROTEIN ANALYSIS; PROTEIN BINDING;

EBOLA VIRUS;

AMINO ACID MOTIFS; BINDING SITES; CELL LINE; DYNEIN ATPASE; EBOLAVIRUS; HUMANS; NUCLEOPROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; VIRAL CORE PROTEINS;

EID: 67449087712     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.00480-09     Document Type: Article

References (35)

1. Akaike, T., and H. Maeda. 2000. Nitric oxide and virus infection. Immunology 101:300-308.
2. Andrejeva, J., K. S. Childs, D. F. Young, T. S. Carlos, N. Stock, S. Goodbourn, and R. E. Randall. 2004. The V proteins of paramyxoviruses bind the IFN-inducible RNA helicase, mda-5, and inhibit its activation of the IFN-beta promoter. Proc. Natl. Acad. Sci. USA 101:17264-17269.
3. Basler, C. F., A. Mikulasova, L. Martinez-Sobrido, J. Paragas, E. Muhlberger, M. Bray, H. D. Klenk, P. Palese, and A. Garcia-Sastre. 2003. The Ebola virus VP35 protein inhibits activation of interferon regulatory factor 3. J. Virol. 77:7945-7956.
4. Basler, C. F., X. Wang, E. Muhlberger, V. Volchkov, J. Paragas, H. D. Klenk, A. Garcia-Sastre, and P. Palese. 2000. The Ebola virus VP35 protein functions as a type I IFN antagonist. Proc. Natl. Acad. Sci. USA 97:12289-12294.
5. Bosio, C. M., M. J. Aman, C. Grogan, R. Hogan, G. Ruthel, D. Negley, M. Mohamadzadeh, S. Bavari, and A. Schmaljohn. 2003. Ebola and Marburg viruses replicate in monocyte-derived dendritic cells without inducing the production of cytokines and full maturation. J. Infect. Dis. 188:1630-1638.
6. Bray, M., K. Davis, T. Geisbert, C. Schmaljohn, and J. Huggins. 1998. A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic fever. J. Infect. Dis. 178:651-661.
7. Bray, M., and T. W. Geisbert. 2005. Ebola virus: the role of macrophages and dendritic cells in the pathogenesis of Ebola hemorrhagic fever. Int. J. Biochem. Cell Biol. 37:1560-1566.
8. Brzozka, K., S. Finke, and K. K. Conzelmann. 2005. Identification of the rabies virus alpha/beta interferon antagonist: phosphoprotein P interferes with phosphorylation of interferon regulatory factor 3. J. Virol. 79:7673-7681.
9. Cardenas, W. B., Y. M. Loo, M. Gale, Jr., A. L. Hartman, C. R. Kimberlin, L. Martinez-Sobrido, E. O. Saphire, and C. F. Basler. 2006. Ebola virus VP35 protein binds double-stranded RNA and inhibits alpha/beta interferon production induced by RIG-I signaling. J. Virol. 80:5168-5178.
10. Chenik, M., M. Schnell, K. K. Conzelmann, and D. Blondel. 1998. Mapping the interacting domains between the rabies virus polymerase and phosphoprotein. J. Virol. 72:1925-1930.
11. Crépieux, P., H. Kwon, N. Leclerc, W. Spencer, S. Richard, R. Lin, and J. Hiscott. 1997. IκBα physically interacts with a cytoskeleton-associated protein through its signal response domain. Mol. Cell. Biol. 17:7375-7385.
12. Dohner, K., C. H. Nagel, and B. Sodeik. 2005. Viral stop-and-go along microtubules: taking a ride with dynein and kinesins. Trends Microbiol. 13:320-327.
13. Enterlein, S., K. L. Warfield, D. L. Swenson, D. A. Stein, J. L. Smith, C. S. Gamble, A. D. Kroeker, P. L. Iversen, S. Bavari, and E. Muhlberger. 2006. VP35 knockdown inhibits Ebola virus amplification and protects against lethal infection in mice. Antimicrob. Agents Chemother. 50:984-993.
14. Geisbert, T. W., L. E. Hensley, T. Larsen, H. A. Young, D. S. Reed, J. B. Geisbert, D. P. Scott, E. Kagan, P. B. Jahrling, and K. J. Davis. 2003. Pathogenesis of Ebola hemorrhagic fever in cynomolgus macaques: evidence that dendritic cells are early and sustained targets of infection. Am. J. Pathol. 163:2347-2370.
15. Greber, U. F., and M. Way. 2006. A superhighway to virus infection. Cell 124:741-754.
16. Hartman, A. L., B. H. Bird, J. S. Towner, Z. A. Antoniadou, S. R. Zaki, and S. T. Nichol. 2008. Inhibition of IRF-3 activation by VP35 is critical for the high level of virulence of Ebola virus. J. Virol. 82:2699-2704.
17. Hartman, A. L., L. Ling, S. T. Nichol, and M. L. Hibberd. 2008. Whole-genome expression profiling reveals that inhibition of host innate immune response pathways by Ebola virus can be reversed by a single amino acid change in the VP35 protein. J. Virol. 82:5348-5358.
18. Jacob, Y., H. Badrane, P. E. Ceccaldi, and N. Tordo. 2000. Cytoplasmic dynein LC8 interacts with lyssavirus phosphoprotein. J. Virol. 74:10217-10222.
19. Jaffrey, S. R., and S. H. Snyder. 1996. PIN: an associated protein inhibitor of neuronal nitric oxide synthase. Science 274:774-777.
20. Kubota, T., M. Matsuoka, T. H. Chang, P. Tailor, T. Sasaki, M. Tashiro, A. Kato, and K. Ozato. 2008. Virus infection triggers SUMOylation of IRF3 and IRF7, leading to the negative regulation of type I interferon gene expression. J. Biol. Chem. 283:25660-25670.
21. Leopold, P. L., and K. K. Pfister. 2006. Viral strategies for intracellular trafficking: motors and microtubules. Traffic 7:516-523.
22. Leroy, E. M., B. Kumulungui, X. Pourrut, P. Rouquet, A. Hassanin, P. Yaba, A. Delicat, J. T. Paweska, J. P. Gonzalez, and R. Swanepoel. 2005. Fruit bats as reservoirs of Ebola virus. Nature 438:575-576.
23. Lo, K. W., H. M. Kan, L. N. Chan, W. G. Xu, K. P. Wang, Z. Wu, M. Sheng, and M. Zhang. 2005. The 8-kDa dynein light chain binds to p53-binding protein 1 and mediates DNA damage-induced p53 nuclear accumulation. J. Biol. Chem. 280:8172-8179.
24. Lo, K. W., S. Naisbitt, J. S. Fan, M. Sheng, and M. Zhang. 2001. The 8-kDa dynein light chain binds to its targets via a conserved (K/R)XTQT motif. J. Biol. Chem. 276:14059-14066.
25. Mahanty, S., and M. Bray. 2004. Pathogenesis of filoviral haemorrhagic fevers. Lancet Infect. Dis. 4:487-498.
26. Mazarakis, N. D., M. Azzouz, J. B. Rohll, F. M. Ellard, F. J. Wilkes, A. L. Olsen, E. E. Carter, R. D. Barber, D. F. Baban, S. M. Kingsman, A. J. Kingsman, K. O'Malley, and K. A. Mitrophanous. 2001. Rabies virus glycoprotein pseudotyping of lentiviral vectors enables retrograde axonal transport and access to the nervous system after peripheral delivery. Hum. Mol. Genet. 10:2109-2121.
27. Mebatsion, T. 2001. Extensive attenuation of rabies virus by simultaneously modifying the dynein light chain binding site in the P protein and replacing Arg333 in the G protein. J. Virol. 75:11496-11502.
28. Muhlberger, E., M. Weik, V. E. Volchkov, H. D. Klenk, and S. Becker. 1999. Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems. J. Virol. 73:2333-2342.
29. Pfister, K. K. 2005. Dynein cargo gets its groove back. Structure 13:172-173.
30. Poisson, N., E. Real, Y. Gaudin, M. C. Vaney, S. King, Y. Jacob, N. Tordo, and D. Blondel. 2001. Molecular basis for the interaction between rabies virus phosphoprotein P and the dynein light chain LC8: dissociation of dynein-binding properties and transcriptional functionality of P. J. Gen. Virol. 82:2691-2696.
31. Rasalingam, P., J. P. Rossiter, T. Mebatsion, and A. C. Jackson. 2005. Comparative pathogenesis of the SAD-L16 strain of rabies virus and a mutant modifying the dynein light chain binding site of the rabies virus phosphoprotein in young mice. Virus Res. 111:55-60.
32. Raux, H., A. Flamand, and D. Blondel. 2000. Interaction of the rabies virus P protein with the LC8 dynein light chain. J. Virol. 74:10212-10216.
33. Sanchez, A., M. P. Kiley, B. P. Holloway, and D. D. Auperin. 1993. Sequence analysis of the Ebola virus genome: organization, genetic elements, and comparison with the genome of Marburg virus. Virus Res. 29:215-240.
34. Tan, G. S., M. A. Preuss, J. C. Williams, and M. J. Schnell. 2007. The dynein light chain 8 binding motif of rabies virus phosphoprotein promotes efficient viral transcription. Proc. Natl. Acad. Sci. USA 104:7229-7234.
35. Wu, H., M. W. Maciejewski, S. Takebe, and S. M. King. 2005. Solution structure of the Tctex1 dimer reveals a mechanism for dynein-cargo interactions. Structure 13:213-223.