A novel, colorimetric neutralization assay for measuring antibodies to influenza viruses

Journal of Virological Methods

Volumn 159, Issue 2, 2009, Pages 271-276

  Lehtoranta, Liisa ^a   Villberg, Anja ^a   Santanen, Riitta ^a   Ziegler, Thedi ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

Author keywords

Antibody response; Cell proliferation assay; Hemagglutination inhibition test; Influenza virus; Neutralization test; Tetrazolium salt

Indexed keywords

FORMAZAN; INACTIVATED VACCINE; INFLUENZA VACCINE; NEUTRALIZING ANTIBODY; OXIDOREDUCTASE; TETRAZOLIUM; TRIPLE VACCINE; VIRUS ANTIBODY;

ANIMAL CELL; ANIMAL CELL CULTURE; ANTIBODY DETECTION; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIBODY TITER; ARTICLE; AUTOMATION; BIOSAFETY; CELL ASSAY; CELL PROLIFERATION; CELL VIABILITY; COLORIMETRY; COMPARATIVE STUDY; CONTROLLED STUDY; HEMAGGLUTINATION INHIBITION TEST; HUMAN; INCUBATION TIME; INFLUENZA VIRUS; NITROBLUE TETRAZOLIUM TEST; NONHUMAN; PRIORITY JOURNAL; SERODIAGNOSIS; SERUM; SPECTROPHOTOMETER; VACCINATION; VIRUS NEUTRALIZATION;

ANIMALS; ANTIBODIES, VIRAL; CELL LINE; CELL SURVIVAL; COLORIMETRY; DOGS; HEMAGGLUTINATION INHIBITION TESTS; HUMANS; NEUTRALIZATION TESTS; ORTHOMYXOVIRIDAE; TETRAZOLIUM SALTS;

ORTHOMYXOVIRIDAE;

EID: 67349268383     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2009.04.015     Document Type: Article

References (18)

1. Bedard J., May S., Barbeau D., Yuen L., Rando R.F., and Bowlin T.L. A high throughput colorimetric cell proliferation assay for the identification of human cytomegalovirus inhibitors. Antiviral Research 41 (1999) 35-43
2. Bright R.A., Carter D.M., Crevar C.J., Toapanta F.R., Steckbeck J.D., Cole K.S., Kumar N.M., Pushko P., Smith G., Tumpey T.M., and Ross T.M. Cross-clade protective immune responses to influenza viruses with H5N1 HA and NA elicited by an influenza virus-like particle. PLoS ONE 3 (2008) e1501
3. Crawford-Miksza L.K., and Schnurr D.P. Quantitative colorimetric microneutralization assay for characterization of adenoviruses. Journal of Clinical Microbiology 32 (1994) 2331-2334
4. Ehrlich H.J., Muller M., Oh H.M.L., Tambyah P.A., Joukhadar C., Montomoli E., Fisher D., Berezuk G., Fritsch S., Low-Baselli A., Vartian N., Bobrovsky R., Pavlova B.G., Pollabauer E.M., Kistner O., Barrett P.N., and Baxter H.N.P.I.V.C.S.T. A clinical trial of a whole-virus H5N1 vaccine derived from cell culture. The New England Journal of Medicine 358 (2008) 2573-2584
5. Harmon M.W., Rota P.A., Walls H.H., and Kendal A.P. Antibody response in humans to influenza virus type B host-cell-derived variants after vaccination with standard (egg-derived) vaccine or natural infection. Journal of Clinical Microbiology 26 (1988) 333-337
6. Heldt C.L., Hernandez R., Mudiganti U., Gurgel P.V., Brown D.T., and Carbonell R.G. A colorimetric assay for viral agents that produce cytopathic effects. Journal of Virological Methods 135 (2006) 56-65
7. Ikonen N., Pyhala R., Axelin T., Kleemola M., and Korpela H. Reappearance of influenza B/Victoria/2/87-lineage viruses: epidemic activity, genetic diversity and vaccination efficacy in the Finnish Defence Forces. Epidemiology and Infection 133 (2005) 263-271
8. Ishiyama M., Shiga M., Sasamoto K., Mizoguchi M., and He P. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chemical and Pharmaceutical Bulletin 41 (1993) 1118-1122
9. Kendal A.P., Pereira M.S., and Skehel J.J. Concepts and Procedures for Laboratory-based Influenza Surveillance, Distributed by the Viral Diseases Unit (1982), Centers for Disease Control and Prevention, Atlanta, GA 30333, USA
10. Leroux-Roels I., Borkowski A., Vanwolleghem T., Dramà M., Clement F.D.R., Hons E., Devaster J.-M., and Leroux-Roels G. Antigen sparing and cross-reactive immunity with an adjuvanted rH5N1 prototype pandemic influenza vaccine: a randomised controlled trial. Lancet 370 9587 (2007) 580-589
11. Matrosovich M., Matrosovich T., Garten W., and Klenk H.-D. New low-viscosity overlay medium for viral plaque assays. Virology Journal 3 (2006) 63
12. Rowe T., Abernathy R.A., Hu-Primmer J., Thompson W.W., Lu X., Lim W., Fukuda K., Cox N.J., and Katz J.M. Detection of antibody to avian influenza A (H5N1) virus in human serum by using a combination of serologic assays. Journal of Clinical Microbiology 37 (1999) 937-943
13. Smee D.F., Morrison A.C., Barnard D.L., and Sidwell R.W. Comparison of colorimetric, fluorometric, and visual methods for determining anti-influenza (H1N1 and H3N2) virus activities and toxicities of compounds. Journal of Virological Methods 106 (2002) 71-79
14. Stephenson I., Das R.G., Wood J.M., and Katz J.M. Comparison of neutralising antibody assays for detection of antibody to influenza A/H3N2 viruses: an international collaborative study. Vaccine 25 (2007) 4056-4063
15. Stephenson I., Wood J.M., Nicholson K.G., Charlett A., and Zambon M.C. Detection of anti-H5 responses in human sera by HI using horse erythrocytes following MF59-adjuvanted influenza A/Duck/Singapore/97 vaccine. Proceedings of the First European Influenza Conference, vol. 103 (2004) 91-95
16. Tannock G.A., Paul J.A., Herd R., Barry R.D., Reid A.L., Hensley M.J., Gillett R.S., Gillett S.M., Lawrance P., and Henry R.L. Improved colorimetric assay for detecting influenza B virus neutralizing antibody responses to vaccination and infection. Journal of Clinical Microbiology 27 (1989) 524-528
17. Treanor J.J., Campbell J.D., Zangwill K.M., Rowe T., and Wolff M. Safety and immunogenicity of an inactivated subvirion influenza A (H5N1) vaccine. The New England Journal of Medicine 354 (2006) 1343-1351
18. Yang X., Chen E., Jiang H., Muszynski K., Harris R.D., Giardina S.L., Gromeier M., Mitra G., and Soman G. Evaluation of IRES-mediated, cell-type-specific cytotoxicity of poliovirus using a colorimetric cell proliferation assay. Journal of Virological Methods 155 (2009) 44-54