E. coli expression of a soluble, active single-chain antibody variable fragment containing a nuclear localization signal

Protein Expression and Purification

Volumn 66, Issue 2, 2009, Pages 172-180

  Xiong, Hairong ^a,d   Li, Shuyi ^a,b   Yang, Zhanqiu ^d   Burgess, Richard R ^c   Dynan, William S ^a  

^a MEDICAL COLLEGE OF GEORGIA   (United States)

^b Apeliotus Technologies Inc   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

^d WUHAN UNIVERSITY   (China)

Author keywords

DNA dependent protein kinase catalytic subunit; Fusion tag; Nuclear localization signal; scFv

Indexed keywords

DNA DEPENDENT PROTEIN KINASE; HYBRID PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CELL NUCLEUS; ESCHERICHIA COLI; GENETICS; HELA CELL; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; METABOLISM; MOLECULAR CLONING; NUCLEAR LOCALIZATION SIGNAL; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STABILITY; SOLUBILITY; WESTERN BLOTTING;

BLOTTING, WESTERN; CELL NUCLEUS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DNA-ACTIVATED PROTEIN KINASE; ESCHERICHIA COLI; HELA CELLS; HUMANS; IMMUNOGLOBULIN VARIABLE REGION; NUCLEAR LOCALIZATION SIGNALS; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN STABILITY; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI;

EID: 67349257383     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.03.002     Document Type: Article

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