Optimizing the yield and utility of circulating cell-free DNA from plasma and serum

Clinica Chimica Acta

Volumn 404, Issue 2, 2009, Pages 100-104

  Xue, Xiaoyan ^a   Teare, M Dawn ^a   Holen, Ingunn ^a   Zhu, Yong M ^a   Woll, Penella J ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Analysis; Cell free DNA; DNA extraction protocol; Methodology; Plasma DNA; Serum DNA

Indexed keywords

CELL FREE DNA; DNA; PROTEINASE; UNCLASSIFIED DRUG;

ARTICLE; BLOOD SAMPLING; DNA EXTRACTION; DNA PURIFICATION; GENE AMPLIFICATION; HUMAN; INTERMETHOD COMPARISON; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION;

BLOOD SPECIMEN COLLECTION; DNA, NEOPLASM; HUMANS; NEOPLASMS; PHENOLS; PROGNOSIS; REAGENT KITS, DIAGNOSTIC;

EID: 67349231169     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2009.02.018     Document Type: Article

References (28)

1. Dhallan R., Guo X., Emche S., et al. A non-invasive test for prenatal diagnosis based on fetal DNA present in maternal blood: a preliminary study. Lancet 369 (2007) 474-481
2. Fleischhacker M., and Schmidt B. Circulating nucleic acids (CNAs) and cancer - a survey. Biochim Biophys Acta 1775 (2007) 181-232
3. Gormally E., Hainaut P., Caboux E., et al. Amount of DNA in plasma and cancer risk: a prospective study. Int J Cancer 111 (2004) 746-749
4. Leon S.A., Shapiro B., Sklaroff D.M., and Yaros M.J. Free DNA in serum of cancer-patients and effect of therapy. Cancer Res 37 (1977) 646-650
5. Sozzi G., Conte D., Mariani L., et al. Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients. Cancer Res 61 (2001) 4675-4678
6. Jahr S., Hentze H., Englisch S., et al. DNA fragments in the blood plasma of cancer patients: quantitations and evidence for their origin from apoptotic and necrotic cells. Cancer Res 61 (2001) 1659-1665
7. Gormally E., Caboux E., Vineis P., and Hainaut P. Circulating free DNA in plasma or serum as biomarker of carcinogenesis: practical aspects and biological significance. Mutat Res 635 (2007) 105-117
8. Tong Y.K., and Lo Y.M.D. Diagnostic developments involving cell-free (circulating) nucleic acids. Clin Chim Acta 363 (2006) 187-196
9. Gautschi O., Bigosch C., Huegli B., et al. Circulating deoxyribonucleic acid as prognostic marker in non-small-cell lung cancer patients undergoing chemotherapy. J Clin Oncol 22 (2004) 4157-4164
10. Xue X., Zhu Y.M., and Woll P.J. Circulating DNA and lung cancer. Ann N Y Acad Sci 1075 (2006) 154-164
11. Khan S., Coulson J.M., and Woll P.J. Genetic abnormalities in plasma DNA of patients with lung cancer and other respiratory diseases. Int J Cancer 110 (2004) 891-895
12. Boddy J.L., Gal S., Malone P.R., Harris A.L., and Wainscoat J.S. Prospective study of quantitation of plasma DNA levels in the diagnosis of malignant versus benign prostate disease. Clin Cancer Res 11 (2005) 1394-1399
13. Wu T.L., Zhang D., Chia J.H., Tsao K.C., Sun C.F., and Wu J.T. Cell-free DNA: measurement in various carcinomas and establishment of normal reference range. Clin Chim Acta 321 (2002) 77-87
14. Chiu R.W.K., Poon L.L.M., Lau T.K., Leung T.N., Wong E.M.C., and Lo Y.M.D. Effects of blood-processing protocols on fetal and total DNA quantification in maternal plasma. Clin Chem 47 (2001) 1607-1613
15. Sozzi G., Roz L., Conte D., et al. Effects of prolonged storage of whole plasma or isolated plasma DNA on the results of circulating DNA quantification assays. J Natl Cancer Inst 97 (2005) 1848-1850
16. de Kok J.B., Hendriks J.C.M., van Solinge W.W., Willems H.L., Mensink E.J., and Swinkels D.W. Use of real-time quantitative PCR to compare DNA isolation methods. Clin Chem 44 (1998) 2201-2204
17. Kramvis A., Bukofzer S., and Kew M.C. Comparison of hepatitis B virus DNA extractions from serum by the QIAamp blood kit, GeneReleaser, and the phenol-chloroform method. J Clin Microbiol 34 (1996) 2731-2733
18. Stemmer C., Beau-Faller M., Pencreac'h E., et al. Use of magnetic beads for plasma cell-free DNA extraction: toward automation of plasma DNA analysis for molecular diagnostics. Clin Chem 49 (2003) 1953-1955
19. Brisson-Noel A., Aznar C., Chureau C., et al. Diagnosis of tuberculosis by DNA amplification in clinical practice evaluation. Lancet 338 (1991) 364-366
20. Schmidt B., Weickmann S., Witt C., and Fleischhacker M. Improved method for isolating cell-free DNA. Clin Chem 51 (2005) 1561-1563
21. Lam N.Y.L., Rainer T.H., Chiu R.W.K., and Lo Y.M.D. EDTA is a better anticoagulant than heparin or citrate for delayed blood processing for plasma DNA analysis. Clin Chem 50 (2004) 256-257
22. Ponchel F., Toomes C., Bransfield K., et al. Real-time PCR based on SYBR-Green I fluorescence: an alternative to the TaqMan assay for a relative quantification of gene rearrangements, gene amplifications and micro gene deletions. BMC Biotechnol 3 (2003)
23. Ebeling W., Hennrich N., Klockow M., Metz H., Orth H.D., and Lang H. Proteinase K from Tritirachium album Limber. Eur J Biochem 47 (1974) 91-97
24. Sozzi G., Conte D., Leon M., et al. Quantification of free circulating DNA as a diagnostic marker in lung cancer. J Clin Oncol 21 (2003) 3902-3908
25. Taback B., O'Day S.J., and Hoon D.S.B. Quantification of circulating DNA in the plasma and serum of cancer patients. Ann N Y Acad Sci 1022 (2004) 17-24
26. Hagiwara N., Mechanic L.E., Trivers G.E., et al. Quantitative detection of p53 mutations in plasma DNA from tobacco smokers. Cancer Res 66 (2006) 8309-8317
27. Lui Y., Chik K.W., Chiu R.W.K., Ho C.Y., Lam C.W.K., and Lo Y.M.D. Predominant hematopoietic origin of cell-free DNA in plasma and serum after sex-mismatched bone marrow transplantation. Clin Chem 48 (2002) 421-427
28. Jung M., Klotzek S., Lewandowski M., Fleischhacker M., and Jung K. Changes in concentration of DNA in serum and plasma during storage of blood samples. Clin Chem 49 (2003) 1028-1029