Functional conversion of fatty acyl-CoA synthetase to firefly luciferase by site-directed mutagenesis: A key substitution responsible for luminescence activity

FEBS Letters

Volumn 583, Issue 12, 2009, Pages 2004-2008

  Oba, Yuichi ^a   Iida, Koichiro ^a   Inouye, Satoshi ^b  

^a NAGOYA UNIVERSITY   (Japan)

^b CHISSO CORPORATION   (Japan)

Author keywords

Acyl CoA ligase; Adenylate forming enzyme; Luciferin binding site; Monooxygenase; Protein evolution

Indexed keywords

LONG CHAIN FATTY ACID COENZYME A LIGASE; LUCIFERASE; LUCIFERIN; SERINE;

AMINO ACID SUBSTITUTION; ARTICLE; BEETLE; CATALYSIS; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; ENZYME ACTIVITY; FIREFLY; GENETIC CONSERVATION; INSECT; LUMINESCENCE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; BEETLES; COENZYME A LIGASES; DROSOPHILA MELANOGASTER; DROSOPHILA PROTEINS; FIREFLY LUCIFERIN; HYDROGEN BONDING; INSECT PROTEINS; LUCIFERASES, FIREFLY; LUMINESCENCE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHYLOGENY; POINT MUTATION; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SERINE;

AGRYPNUS BINODULUS; COLEOPTERA; DROSOPHILA MELANOGASTER; ELATERIDAE; HEXAPODA;

EID: 67349177132     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.febslet.2009.05.018     Document Type: Article

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