Diagnostic value of HCMV pp65 antigen detection by FCA for symptomatic and asymptomatic infection: Compared to quantification of HCMV DNA and detection of IgM antibody in infants

Medical Microbiology and Immunology

Volumn 198, Issue 2, 2009, Pages 107-112

  Sun, Zhengrong ^a   Ceng, Xiangshi ^a   Mao, Zhiqin ^a   Wang, Jidong ^a   Liu, Qing ^a   Ji, Yaohua ^a   Ma, Yanping ^a   Qi, Ying ^a   He, Rong ^a   Ruan, Qiang ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

Flow cytometry; HCMV; IgM antibody; PP65Ag; Real time PCR

Indexed keywords

CYTOMEGALOVIRUS ANTIGEN PP65; IMMUNOGLOBULIN M ANTIBODY; VIRUS ANTIGEN; VIRUS DNA;

ANTIBODY DETECTION; ARTICLE; BLOOD SAMPLING; CHEMOLUMINESCENCE; CHILD; CONTROLLED STUDY; CYTOMEGALOVIRUS INFECTION; DIAGNOSTIC VALUE; FLOW CYTOMETRY; HUMAN; HUMAN CYTOMEGALOVIRUS; IMMUNOASSAY; INFANT; LABORATORY DIAGNOSIS; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROGNOSIS; QUANTITATIVE ANALYSIS; SYMPTOM; URINALYSIS;

ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; FLOW CYTOMETRY; HUMANS; IMMUNOGLOBULIN M; INFANT; PHOSPHOPROTEINS; PREDICTIVE VALUE OF TESTS; VIRAL MATRIX PROTEINS;

EID: 67349157199     PISSN: 03008584     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00430-009-0112-y     Document Type: Article

References (22)

1. AM Arvin P Fast M Myers S Plotkin R Rabinovich 2004 Vaccine development to prevent cytomegalovirus disease: report from the National Vaccine Advisory Committee Clin Infect Dis 39 233 239 10.1086/421999
2. MJ Cannon KF Davis 2005 Washing our hands of the congenital cytomegalovirus disease epidemic BMC Public Health 5 70 10.1186/1471-2458-5-70
3. M Boeckh GM Gallez-Hawkins D Myerson JA Zaia RA Bowden 1997 Plasma polymerase chain reaction for cytomegalovirus DNA after allogeneic marrow transplantation: comparison with polymerase chain reaction using peripheral blood leukocytes, pp65 antigenemia, and viral culture Transplantation 64 1 108 113 10.1097/00007890-199707150-00020
4. E Petersen MV Borobio E Guy O Liesenfeld V Meroni A Naessens 2005 European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index J Clin Microbiol 43 4 1570 1574 10.1128/JCM.43.4.1570- 1574.2005
5. G Gerna MG Revello E Percivalle F Morina 1992 Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia J Clin Microbiol 30 1232
6. V Ghisetti A Barbui E Donegani M Bobbio P Caimmi S Pansini 1996 Comparison of polymerase chain reaction and pp65 antigen test for early detection of human cytomegalovirus in blood leukocytes of cardiac transplant recipients Clin Microbiol Infect 1 195 10.1111/j.1469-0691.1996.tb00553.x
7. OL Laskin CM Stahl-bayliss CM Kalman LR Rosecan 1999 Diagnostic and prognostic value of human cytomegalovirus load and IgM antibody in blood of congenitally infected infants J Clin Virol 14 1 57 66 10.1016/S1386-6532(99) 00016-5
8. JJ Masharry 1994 Uses of flow cytometry in virology Clin Microbiol Rev 7 576
9. JJ Ploem-Zaaijer WE Mesker GJ Boland WC Sloos FM van de Rijke M Jiwa 1994 Automated image cytometry for detection of rare viral antigen-positive cells in peripheral blood Cytometry 15 199 10.1002/cyto.990150304
10. BM Imbert-Marcille N Robillard AS Poirier M Coste-Burel D Cantarovich N Milpied 1997 Development of a method for direct quantification of cytomegalovirus antigenemia by flow cytometry J Clin Microbiol 35 10 2665
11. N Jiwa W Mesker J Ploem-zaaijer W Van drop T The A Raap 1994 Quantification of low frequency white blood cells expressing human cytomegalovirus antigen by image cytometry Cytometry 16 69 10.1002/cyto. 990160110
12. SB Boppana RF Pass WJ Britt S Stagno CA Alford 1992 Symptomatic congenital cytomegalovirus infection: neonatal morbidity and mortality Pediatr Infect Dis J 11 93 99 10.1097/00006454-199202000-00007
13. IG Sia R Patel 2000 New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients Clin Microbiol Rev 13 1 83 121
14. CV Paya 2002 Prevention of fungal infection in transplantation Transpl Infect Dis 4 Suppl 3 46 51 10.1034/j.1399-3062.4.s3.7.x
15. K St George MJ Boyd SM Lipson D Ferguson GF Cartmell LH Falk 2000 A multisite trial comparing two cytomegalovirus (CMV) pp65 antigenemia test kits, biotest CMV brite and Bartels/Argene CMV antigenemia J Clin Microbiol 38 4 1430 1433
16. L von Muller J Hinz M Bommer W Hampl S Kluwick M Wiedmann 2007 CMV monitoring using blood cells and plasma: a comparison of apples with oranges? Bone Marrow Transplant 39 353 357 10.1038/sj.bmt.1705593
17. G May JE Kühn BR Eing 2006 Comparison of two commercially available pp65 antigenemia tests and COBAS Amplicor CMV Monitor for early detection and quantification of episodes of human CMV-viremia in transplant recipients Intervirology 49 261 265 10.1159/000093455
18. S Kusne P Grossi W Irish K St George C Rinaldo J Rakela 1999 Cytomegalovirus pp65 antigenemia monitoring as a guide for preemptive therapy: a cost effective strategy for prevention of cytomegalovirus disease in adult liver transplant recipient Transplantation 68 1125 1131 10.1097/00007890- 199910270-00011
19. IG Sia R Patel 2000 New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients Clin Microbiol Rev 13 83 121
20. SS Kalpoe ACM Kroes MD de Jong J Schinkel CS de Brouwer MFC Beersma 2004 Validation of clinical application of cytomegalovirus plasma DNA load measurement and definition of treatment criteria by analysis of correlation to antigen detection J Clin Microbiol 42 1498 1504 10.1128/JCM.42.4.1498-1504.2004
21. C Mengelle C Pasquier L Rostaing K Sandres-Sauné J Puel L Berges 2003 Quantitation of human cytomegalovirus in recipients of solid organ transplants by real-time quantitative PCR and pp65 antigenemia J Med Virol 69 225 231 10.1002/jmv.10277
22. A Nitsche O Oswald N Steuer J Schetelig A Radonić S Thulke 2003 Quantitative real-time PCR compared with pp65 antigen detection for cytomegalovirus (CMV) in 1122 blood specimens from 77 patients after allogeneic stem cell transplantation: which test better predicts CMV disease development? Clin Chem 49 1683 1685 10.1373/49.10.1683