Quantitative real-time PCR compared with pp65 antigen detection for cytomegalovirus (CMV) in 1122 blood specimens from 77 patients after allogeneic stem cell transplantation: Which test better predicts CMV disease development?

Clinical Chemistry

Volumn 49, Issue 10, 2003, Pages 1683-1685

  Nitsche, Andreas ^a,b   Oswald, Olivia ^a   Steuer, Nina ^a   Schetelig, Johannes ^a   Radonić, Aleksandar ^a   Thulke, Stefanie ^a   Siegert, Wolfgang ^a,c  

^a HUMBOLDT UNIVERSITY   (Germany)

^b ROBERT KOCH INSTITUTE   (Germany)

^c CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ALLOTRANSPLANTATION; ANTIGEN DETECTION; ARTICLE; CONTROLLED STUDY; CORRELATION ANALYSIS; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTION; DIAGNOSTIC ACCURACY; DIAGNOSTIC ERROR; DIAGNOSTIC TEST; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PREDICTION; STEM CELL TRANSPLANTATION; VIRUS LOAD;

CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; DNA, VIRAL; HUMANS; IMMUNOASSAY; LEUKOCYTES; PHOSPHOPROTEINS; POLYMERASE CHAIN REACTION; PROGNOSIS; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; STEM CELL TRANSPLANTATION; VIRAL LOAD; VIRAL MATRIX PROTEINS;

CUCUMBER MOSAIC VIRUS; CYTOMEGALOVIRUS;

EID: 0141727611     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1373/49.10.1683     Document Type: Article

References (15)

1. Schmidt CA, Oettle H, Neuhaus P, Wiens M, Timm H, Wilborn F, et al. Demonstration of cytomegalovirus by polymerase chain reaction after liver transplantation 1993;56:872-4.
2. Schmidt CA, Oettle H, Peng R, Neuhaus P, Blumhardt G, Lohmann R, et al. Comparison of polymerase chain reaction from plasma and buffy coat with antigen detection and occurrence of immunoglobulin M for the demonstration of cytomegalovirus infection after liver transplantation. Transplantation 1995;59:1133-8.
3. Schmidt CA, Oettle H, Wilborn F, Jessen J, Timm H, Schwerdtfeger R, et al. Demonstration of cytomegalovirus after bone marrow transplantation by polymerase chain reaction, virus culture and antigen detection in buffy coat leukocytes. Bone Marrow Transplant 1994;13:71-5.
4. Gerna G, Percivalle E, Torsellini M, Revello MG. Standardization of the human cytomegalovirus antigenemia assay by means of in vitro-generated pp65-positive peripheral blood polymorphonuclear leukocytes. J Clin Microbiol 1998;36:3585-9.
5. Boeckh M, Woogerd PM, Stevens-Ayers T, Ray CG, Bowden RA. Factors influencing detection of quantitative cytomegalovirus antigenemia. J Clin Microbiol 1994;32:832-4.
6. Nitsche A, Steuer N, Schmidt CA, Landt O, Ellerbrok H, Pauli G, et al. Detection of human cytomegalovirus DNA by real-time quantitative PCR. J Clin Microbiol 2000;38:2734-7.
7. Nitsche A, Steuer N, Schmidt CA, Landt O, Siegert W. Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA. Clin Chem 1999;45:1932-7.
8. Li H, Dummer JS, Estes WR, Meng S, Wright PF, Tang YW. Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients. J Clin Microbiol 2003;41:187-91.
9. Tanaka Y, Kanda Y, Kami M, Mori S, Hamaki T, Kusumi E, et al. Monitoring cytomegalovirus infection by antigenemia assay and two distinct plasma real-time PCR methods after hematopoietic stem cell transplantation. Bone Marrow Transplant 2002;30:315-9.
10. Yakushiji K, Gondo H, Kamezaki K, Shigematsu K, Hayashi S, Kuroiwa M, et al. Monitoring of cytomegalovirus reactivation after allogeneic stem cell transplantation: comparison of an antigenemia assay and quantitative real-time polymerase chain reaction. Bone Marrow Transplant 2002;29:599-606.
11. Griscelli F, Barrois M, Chauvin S, Lastere S, Bellet D, Bourhis JH. Quantification of human cytomegalovirus DNA in bone marrow transplant recipients by real-time PCR. J Clin Microbiol 2001;39:4362-9.
12. Leruez-Ville M, Ouachee M, Delarue R, Sauget AS, Blanche S, Buzyn A, et al. Monitoring cytomegalovirus infection in adult and pediatric bone marrow transplant recipients by a real-time PCR assay performed with blood plasma. J Clin Microbiol 2003;41:2040-6.
13. Boivin G, Belanger R, Delage R, Beliveau C, Demers C, Goyette N, et al. Quantitative analysis of cytomegalovirus (CMV) viremia using the pp65 antigenemia assay and the COBAS AMPLICOR CMV MONITOR PCR test after blood and marrow allogeneic transplantation. J Clin Microbiol 2000;38:4356-60.
14. Barrett-Muir W, Breuer J, Millar C, Thomas J, Geffries D, Yaqoob M, et al. CMV viral load measurements in whole blood and plasma - which is best following renal transplantation? Transplantation 2000;70:116-9.
15. Schafer P, Tenschert W, Cremaschi L, Schroter M, Zollner B, Laufs R. Area under the viraemia curve versus absolute viral load: utility for predicting symptomatic cytomegalovirus infections in kidney transplant patients. J Med Virol 2001;65:85-9.