Toxoplasma gondii: Over-expression of lactate dehydrogenase enhances differentiation under alkaline conditions

Experimental Parasitology

Volumn 122, Issue 2, 2009, Pages 155-161

  Liwak, Urszula ^a   Ananvoranich, Sirinart ^a  

^a UNIVERSITY OF WINDSOR   (Canada)

Author keywords

Differentiation; Enzymatic activity; Lactate dehydrogenase; Toxoplasma gondii

Indexed keywords

GREEN FLUORESCENT PROTEIN; LACTATE DEHYDROGENASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GROWTH; BRADYZOITE; CELL DIFFERENTIATION; CYST (RESTING STAGE); ENZYME ACTIVITY; GENE OVEREXPRESSION; GROWTH CURVE; IMMUNOFLUORESCENCE TEST; IN VITRO STUDY; NONHUMAN; PARASITE; PARASITE VIRULENCE; PRIORITY JOURNAL; TACHYZOITE; TOXOPLASMA GONDII; WESTERN BLOTTING;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BLOTTING, WESTERN; CELLS, CULTURED; FIBROBLASTS; FLUORESCENT ANTIBODY TECHNIQUE; FORESKIN; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; HYDROGEN-ION CONCENTRATION; L-LACTATE DEHYDROGENASE; MALE; RECOMBINANT PROTEINS; TOXOPLASMA;

TOXOPLASMA GONDII;

EID: 67349128038     PISSN: 00144894     EISSN: 10902449     Source Type: Journal    
DOI: 10.1016/j.exppara.2009.01.016     Document Type: Article

References (22)

1. Al-Anouti F., Tomavo S., Parmley S., and Ananvoranich S. The expression of lactate dehydrogenase is important for the cell cycle of Toxoplasma gondii. Journal of Biological Chemistry 279 (2004) 52300-52311
2. Bohne W., Gross U., Ferguson D.J., and Heesemann J. Cloning and characterization of a bradyzoite-specifically expressed gene (hsp30/bag1) of Toxoplasma gondii, related to genes encoding small heat-shock proteins of plants. Molecular Microbiology 16 (1995) 1221-1230
3. Boothroyd J.C., Black M., Bonnefoy S., Hehl A., Knoll L.J., Manger I.D., Ortega-Barria E., and Tomavo S. Genetic and biochemical analysis of development in Toxoplasma gondii. Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences 352 (1997) 1347-1354
4. Dando C., Schroeder E.R., Hunsaker L.A., Deck L.M., Royer R.E., Zhou X., Parmley S.F., and Vander Jagt D.L. The kinetic properties and sensitivities to inhibitors of lactate dehydrogenases (LDH1 and LDH2) from Toxoplasma gondii: comparisons with pLDH from Plasmodium falciparum. Molecular and Biochemical Parasitology 118 (2001) 23-32
5. Denton H., Roberts C.W., Alexander J., Thong K.W., and Coombs G.H. Enzymes of energy metabolism in the bradyzoites and tachyzoites of Toxoplasma gondii. FEMS Microbiology Letters 137 (1996) 103-108
6. Donald R.G., Carter D., Ullman B., and Roos D.S. Insertional tagging, cloning, and expression of the Toxoplasma gondii hypoxanthine-xanthine-guanine phosphoribosyltransferase gene. Use as a selectable marker for stable transformation. Journal of Biological Chemistry 271 (1996) 14010-14019
7. Ferguson D.J., Parmley S.F., and Tomavo S. Evidence for nuclear localisation of two stage-specific isoenzymes of enolase in Toxoplasma gondii correlates with active parasite replication. International Journal for Parasitology 32 (2002) 1399-1410
8. Fleige T., Fischer K., Ferguson D.J., Gross U., and Bohne W. Carbohydrate metabolism in the Toxoplasma gondii apicoplast: localization of three glycolytic isoenzymes, the single pyruvate dehydrogenase complex, and a plastid phosphate translocator. Eukaryotic Cell 6 (2007) 984-996
9. Kavanagh K.L., Elling R.A., and Wilson D.K. Structure of Toxoplasma gondii LDH1: active-site differences from human lactate dehydrogenases and the structural basis for efficient APAD+ use. Biochemistry 43 (2004) 879-889
10. Khan A., Taylor S., Su C., Mackey A.J., Boyle J., Cole R., Glover D., Tang K., Paulsen I.T., Berriman M., Boothroyd J.C., Pfefferkorn E.R., Dubey J.P., Ajioka J.W., Roos D.S., Wootton J.C., and Sibley L.D. Composite genome map and recombination parameters derived from three archetypal lineages of Toxoplasma gondii. Nucleic Acids Research 33 (2005) 2980-2992
11. Kiefhaber T., Rudolph R., Kohler H.H., and Buchner J. Protein aggregation in vitro and in vivo: a quantitative model of the kinetic competition between folding and aggregation. Biotechnology 9 (1991) 825-829
12. Lindsay D.S., Dubey J.P., Blagburn B.L., and Toivio-Kinnucan M. Examination of tissue cyst formation by Toxoplasma gondii in cell cultures using bradyzoites, tachyzoites, and sporozoites. Journal of Parasitology 77 (1991) 126-132
13. Mukhopadhyay A. Inclusion bodies and purification of proteins in biologically active forms. Advances in Biochemical Engineering/Biotechnology 56 (1997) 61-109
14. Reiter-Owona I., Seitz H., Gross U., Sahm M., Rockstroh J.K., and Seitz H.M. Is stage conversion the initiating event for reactivation of Toxoplasma gondii in brain tissue of AIDS patients?. Journal of Parasitology 86 (2000) 531-536
15. Roos D.S., Donald R.G., Morrissette N.S., and Moulton A.L. Molecular tools for genetic dissection of the protozoan parasite Toxoplasma gondii. Methods in Cell Biology 45 (1994) 27-63
16. Soete M., Fortier B., Camus D., and Dubremetz J.F. Toxoplasma gondii: kinetics of bradyzoite-tachyzoite interconversion in vitro. Experimental Parasitology 76 (1993) 259-264
17. Striepen B., He C.Y., Matrajt M., Soldati D., and Roos D.S. Expression, selection, and organellar targeting of the green fluorescent protein in Toxoplasma gondii. Molecular and Biochemical Parasitology 92 (1998) 325-338
18. Tomavo S. The differential expression of multiple isoenzyme forms during stage conversion of Toxoplasma gondii: an adaptive developmental strategy. International Journal for Parasitology 31 (2001) 1023-1031
19. Van T.T., Kim S.K., Camps M., Boothroyd J.C., and Knoll L.J. The BSR4 protein is up-regulated in Toxoplasma gondii bradyzoites, however the dominant surface antigen recognised by the P36 monoclonal antibody is SRS9. International Journal for Parasitology 37 (2007) 877-885
20. Weiss L.M., and Kim K. The development and biology of bradyzoites of Toxoplasma gondii. Frontiers in Bioscience 5 (2000) D391-D405
21. Yang S., and Parmley S.F. A bradyzoite stage-specifically expressed gene of Toxoplasma gondii encodes a polypeptide homologous to lactate dehydrogenase. Molecular and Biochemical Parasitology 73 (1995) 291-294
22. Yang S., and Parmley S.F. Toxoplasma gondii expresses two distinct lactate dehydrogenase homologous genes during its life cycle in intermediate hosts. Gene 184 (1997) 1-12