A cell-based assay for screening of antidotes to, and antivenom against Chironex fleckeri (box jellyfish) venom

Journal of Pharmacological and Toxicological Methods

Volumn 59, Issue 3, 2009, Pages 166-170

  Konstantakopoulos, Nicki ^a   Isbister, Geoffrey K ^a,b,c   Seymour, Jamie E ^d   Hodgson, Wayne C ^a  

^a MONASH UNIVERSITY   (Australia)

^b MENZIES SCHOOL OF HEALTH RESEARCH   (Australia)

^c UNIVERSITY OF NEWCASTLE   (Australia)

^d JAMES COOK UNIVERSITY   (Australia)

Author keywords

Antivenom; Aortic smooth muscle; Box jellyfish; Cell viability; Cell based assay; Chironex fleckeri; Venom

Indexed keywords

ANTIDOTE; CALCIUM; FELODIPINE; JELLYFISH ANTIVENOM; LANTHANUM; MAGNESIUM SULFATE; UNCLASSIFIED DRUG; VENOM; VERAPAMIL;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL BASED ASSAY; CELL PROLIFERATION; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; IC 50; IN VIVO STUDY; JELLYFISH; NONHUMAN; RAT; TOXIN ANALYSIS;

ANIMALS; ANTIDOTES; ANTIVENINS; AORTA; BIOLOGICAL ASSAY; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CNIDARIAN VENOMS; CUBOZOA; FELODIPINE; MAGNESIUM SULFATE; MUSCLE, SMOOTH, VASCULAR; MYOCYTES, SMOOTH MUSCLE; RATS; VERAPAMIL;

ANIMALIA; CHIRONEX FLECKERI; CUBOZOA; RATTUS;

EID: 67349109814     PISSN: 10568719     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vascn.2009.02.003     Document Type: Article

References (34)

1. 2+, haemolysis and Artemia sp. lethality. Toxicon 45 (2005) 233-242
2. Baxter E.H., and Marr G.M. Sea wasp (Chironex fleckeri) antivenene: Neutralising potency against the venom of three other jellyfish species. Toxicon 12 (1974) 223-229
3. Beadnell C.E., Rider T.A., Williamson J.A., and Fenner P.J. Management of a major box jellyfish (Chironex fleckeri) sting. Lessons from the first minutes and hours. Medical Journal of Australia 156 (1992) 655-658
4. Bloom D.A., Burnett J.W., and Alderslade P. Partial purification of box jellyfish (Chironex fleckeri) nematocyst venom isolated at the beachside. Toxicon 36 (1998) 1075-1085
5. Bloom D.A., Burnett W., Hebel J.R., and Alderslade P. Effects of verapamil and CSL antivenom on Chironex fleckeri (box-jellyfish) induced mortality. Toxicon 37 (1999) 1621-1626
6. Brinkman D., and Burnell J. Identification, cloning and sequencing of two major venom proteins from the box jellyfish, Chironex fleckeri. Toxicon 50 (2007) 850-860
7. Burnett J.W., and Calton G.J. Response of the box-jellyfish (Chironex fleckeri) cardiotoxin to intravenous administration of verapamil. Medical Journal of Australia 2 (1983) 192-194
8. Burnett J.W., Othman I.B., Endean R., Fenner P.J., Callanan V.I., and Williamson J.A. Verapamil potentiation of Chironex (box-jellyfish) antivenom. Toxicon 28 (1990) 242-244
9. Carrette T., and Seymour J. A rapid and repeatable method for venom extraction from Cubozoan nematocysts. Toxicon 44 (2004) 135-139
10. Corkeron M.A. Magnesium infusion to treat Irukandji syndrome. Medical Journal of Australia 178 (2003) 411
11. Corkeron M., Pereira P., and Makrocanis C. Early experience with magnesium administration in Irukandji syndrome. Anaesthesia and Intensive Care 32 (2004) 666-669
12. Currie B. Clinical implications of research on the box-jellyfish Chironex fleckeri. Toxicon 32 (1994) 1305-1313
13. Currie B.J. Clinical toxicology: A tropical Australian perspective. Therapeutic Drug Monitoring 22 (2000) 73-78
14. Endean R., and Henderson L. Further studies of toxic material from nematocysts of the cubomedusan Chironex fleckeri Southcott. Toxicon 7 (1969) 303-314
15. Endean R., and Sizemore D.J. The effectiveness of antivenom in countering the actions of box-jellyfish (Chironex fleckeri) nematocyst toxins in mice. Toxicon 26 (1988) 425-431
16. Fawcett W.J., Haxby E.J., and Male D.A. Magnesium: Physiology and pharmacology. British Journal of Anaesthesia 83 (1999) 302-320
17. Freeman S.E., and Turner R.J. Cardiovascular effects of toxins isolated from the cnidarian Chironex fleckeri Southcott. British Journal of Pharmacology 41 (1971) 154-166
18. Jacob T., Ascher E., Hingorani A., and Kallakuri S. The effect of von Hippel-Lindau gene transfer on human vascular smooth muscle cell proliferation and apoptosis. Vascular and Endovascular Surgery 39 (2005) 25-32
19. Jing Q., Xin S.M., Cheng Z.J., Zhang W.B., Zhang R., Qin Y.W., et al. Activation of p38 mitogen-activated protein kinase by oxidized LDL in vascular smooth muscle cells: Mediation via pertussis toxin-sensitive G proteins and association with oxidized LDL-induced cytotoxicity. Circulation Research 84 (1999) 831-839
20. Leon C., Taylor R., Bartlett K.H., and Wasan K.M. Effect of heat-treatment and the role of phospholipases on Fungizone-induced cytotoxicity within human kidney proximal tubular (HK-2) cells and Aspergillus fumigatus. International Journal of Pharmaceutics 298 (2005) 211-218
21. Lumley J., Williamson J.A., Fenner P.J., Burnett J.W., and Colquhoun D.M. Fatal envenomation by Chironex fleckeri, the north Australian box jellyfish: The continuing search for lethal mechanisms. Medical Journal of Australia 148 (1988) 527-533
22. Ma Z.C., Gao Y., Wang Y.G., Tan H.L., Xiao C.R., and Wang S.Q. Ginsenoside Rg1 inhibits proliferation of vascular smooth muscle cells stimulated by tumor necrosis factor-alpha. Acta Pharmacologica Sinica 27 (2006) 1000-1006
23. Maguire E.J. Chironex fleckeri ("sea wasp") sting. Medical Journal of Australia 2 (1968) 1137-1138
24. Mustafa M.R., White E., Hongo K., Othman I., and Orchard C.H. The mechanism underlying the cardiotoxic effect of the toxin from the jellyfish Chironex fleckeri. Toxicology and Applied Pharmacology 133 (1995) 196-206
25. 2 inhibits rat vascular smooth muscle cell proliferation stimulated by advanced glycation end products. Di Yu Jun Yi Da Xue Xue Bao 23 (2003) 210-212
26. Ramasamy S., Isbister G.K., Seymour J.E., and Hodgson W.C. The in vitro effect of two chirodropid (Chironex fleckeri and Chiropsalmus sp) venoms: Efficacy of box jellyfish antivenom. Toxicon 41 (2003) 703-711
27. Ramasamy S., Isbister G.K., Seymour J.E., and Hodgson W.C. The in vivo cardiovascular effects of box jellyfish Chironex fleckeri venom in rats: Efficacy of pre-treatment with antivenom, verapamil and magnesium sulphate. Toxicon 43 (2004) 685-690
28. Ramasamy S., Isbister G.K., Seymour J.E., and Hodgson W.C. The in vivo cardiovascular effects of an Australian box jellyfish (Chiropsalmus sp) venom in rats. Toxicon 45 (2005) 321-327
29. Tibballs J., Williams D., and Sutherland S.K. The effects of antivenom and verapamil on the haemodynamic actions of Chironex fleckeri (box jellyfish) venom. Anaesthesia and Intensive Care 26 (1998) 40-45
30. Triggle D.J. L-type calcium channels. Current Pharmaceutical Design 12 (2006) 443-457
31. Williamson J.A., LeRay L.E., Wohlfart M., and Fenner P.J. Acute management of serious envenomation by box-jellyfish (Chironex fleckeri). Medical Journal of Australia 141 (1984) 851-853
32. Winter K.L., Isbister G.K., Jacoby T., Seymour J.E., and Hodgson W.C. An in vivo comparison of the efficacy of CSL box jellyfish antivenom with antibodies raised against nematocyst-derived Chironex fleckeri venom. Toxicology Letters 187 (2009) 94-98 10.1016/j.toxlet.2009.02.008
33. Winter K.L., Isbister G.K., Ramasamy S., Fernando R., Seymour J., and Hodgson W.C. The in vitro vascular effects of two Chirodropid (Chironex fleckeri and Chiropsella bronzie) venoms. Toxicology Letters 168 (2007) 13-20
34. Yang M., Huang H.L., Zhu B.Y., Tuo Q.H., and Liao D.F. Onychin inhibits proliferation of vascular smooth muscle cells by regulating cell cycle. Acta pharmacologica Sinica 26 (2005) 205-211