Purification, characterization and biotechnological application of an alkaline β-keratinase produced by Bacillus subtilis RM-01 in solid-state fermentation using chicken-feather as substrate

Biochemical Engineering Journal

Volumn 45, Issue 3, 2009, Pages 218-225

  Rai, Sudhir K ^a   Konwarh, Rocktotpal ^a   Mukherjee, Ashis K ^a  

^a TEZPUR UNIVERSITY   (India)

Author keywords

Bioreactor systems; Enzyme; Keratinase; Microbial; Response surface method; Solid state; Waste treatment

Indexed keywords

BACILLUS SUBTILIS; BACILLUS SUBTILIS STRAINS; BIOREACTOR SYSTEMS; BIOTECHNOLOGICAL APPLICATIONS; CULTURE CONDITIONS; DISTILLED WATER; KERATINASE; LAUNDRY DETERGENTS; MEDIA COMPOSITION; MICROBIAL; NITROGEN SOURCES; OPTIMIZED CONDITIONS; RESPONSE SURFACE METHOD; RESPONSE SURFACE METHODOLOGY; SERINE PROTEASE; SODIUM NITRATES; SOLID-STATE FERMENTATION; SUBSTRATE SPECIFICITY;

AMINO ACIDS; BACTERIOLOGY; BIOCHEMICAL ENGINEERING; BIOLOGICAL WATER TREATMENT; BIOREACTORS; CLEANING; ENZYME ACTIVITY; ENZYMES; FERMENTATION; INDUSTRIAL CHEMICALS; INDUSTRIAL WASTE TREATMENT; MEATS; OPTIMIZATION; PURIFICATION; SOAPS (DETERGENTS); SODIUM; SURFACE ACTIVE AGENTS; SURFACE PROPERTIES;

SUBSTRATES;

BETA KERATINASE; DETERGENT; FERROUS ION; KERATINASE; MALTOSE; SERINE PROTEINASE; SODIUM NITRATE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BACILLUS SUBTILIS; CHICKEN; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SYNTHESIS; FEATHER; FERMENTATION OPTIMIZATION; INCUBATION TIME; MOLECULAR WEIGHT; NONHUMAN; PRIORITY JOURNAL; RECYCLING; RESPONSE SURFACE METHOD; SOLID STATE FERMENTATION;

BACILLUS SUBTILIS;

EID: 67349101690     PISSN: 1369703X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bej.2009.04.001     Document Type: Article

References (36)

1. Williams C.M., Richter C.S., Mackenzie J.M., and Shih J.C.H. Isolation, identification and characterization of a feather-degrading bacterium. Appl. Environ. Microbiol. 56 (1990) 1509-1515
2. Friedrich A.B., and Antranikian G. Keratin degradation by Fervidobacterium pennavorans, a novel thermophilic anaerobic species of the order thermotogales. Appl. Environ. Microbiol. 62 (1996) 2875-2882
3. De Toni C.H., Richter M.F., Chagas J.R., Henriques J.A.P., and Termignoni C. Purification and characterization of an alkaline serine endopeptidase from a feather-degrading Xanthomonas maltophila strain. Can. J. Microbiol. 48 (2002) 342-348
4. Holker U., and Lenz J. Solid-state fermentation-are there any biotechnological advantages?. Curr. Opin. Microbiol. 8 (2005) 301-306
5. Das K., and Mukherjee A.K. Comparison of lipopeptide biosurfactants production by Bacillus subtilis strains in submerged and solid state fermentation systems using a cheap carbon source: some industrial applications of biosurfactants. Process Biochem. 42 (2007) 1191-1199
6. Mukherjee A.K., Adhikari H., and Rai S.K. Production of alkaline protease by a thermophilic Bacillus subtilis under solid-state fermentation (SSF) condition using Imperata cylindrica grass and potato peel as low-cost medium: characterization and application of enzyme in detergent formulation. Biochem. Eng. J. 39 (2008) 353-361
7. Barrios-Gonzalez J., Castillo T.E., and Mejia A. Development of high penicillin-producing strains for solid state fermentation. Biotechnol. Adv. 11 (1993) 525-537
8. Brandelli A., and Riffel A. Production of an extracellular keratinase from Chryseobacterium sp. growing on raw feathers. Elect. J. Biotechnol. 8 (2005) 35-40
9. Cappuccino J.G., and Sherman N. Microbiology-A Laboratory Manual (1999), Addison-Wesley Longman, Inc.
10. Mukherjee A.K., and Das K. Correlation between diverse cyclic lipopeptides production and regulation of growth and substrate utilization by Bacillus subtilis strains in a particular habitat. FEMS Microbiol. Ecol. 54 (2005) 479-489
11. Haaland P.D. Experimental Design in Biotechnology (1990), Elsevier Science Publishing Co., New York, USA
12. Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J. Protein measurement with the folin-phenol reagent. J. Biol. Chem. 193 (1951) 267-275
13. Laemmli U.K. Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
14. Das K., and Mukherjee A.K. Characterization of biochemical properties and biological activities of biosurfactants produced by Pseudomonas aeruginosa mucoid and non-mucoid strains isolated from hydrocarbon-contaminated soil sample. Appl. Microbiol. Biotechnol. 69 (2005) 192-199
15. Raimbault M., and Alazard D. Culture method to study fungi growth in solid fermentation. Appl. Microbiol. Biotechnol. 9 (1980) 199-209
16. Ikasari L., and Mitchelli D.A. Leaching and characterization of Rhizopus oligosporous acid protease from solid-state fermentation. Enz. Microb. Technol. 19 (1994) 171-175
17. Voet D., and Voet J.G. Three-dimensional structures of proteins. Biochemistry. 3rd edition (2004), John Wiley and Sons, Inc. pp. 219-275
18. Anbu P., Gopinath S.C.B., Hilda A., Lakshmipriya T., and Annadurai G. Optimization of extracellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis. Bioresour. Technol. 98 (2007) 1298-1303
19. El-Refai H.A., AbdelNaby M.A., Gaballa A., El-Araby M.H., and Abdel Fattah A.F. Improvement of the newly isolated Bacillus pumilus FH9 keratinolytic activity. Process Biochem. 40 (2005) 2325-2332
20. Gupta R., Beg Q.K., and Lorenz P. Bacterial alkaline proteases: molecular approaches and industrial applications. Appl. Microbiol. Biotechnol. 59 (2002) 15-32
21. Gessesse A., Hatti-Kaul R., Gashe B.A., and Mattiasson B. Novel alkaline proteases from alkaliphilic bacteria grown on chicken feather. Enz. Microb. Technol. 32 (2003) 519-524
22. Prakasham R.S., Rao S.C.H., and Sarma P.N. Green gram husk-an inexpensive substrate for alkaline protease production by Bacillus sp. in solid-state fermentation. Bioresour. Technol. 97 (2006) 1449-1454
23. Kole M.M., Draper L., and Gerson D.F. Production of protease by Bacillus subtilis using simultaneous control of glucose and ammonium concentration. J. Chem. Technol. Biotechnol. 41 (1998) 197-206
24. Frankena J., Koningstein C.M., Van verseveld H.W., and Stouthamer A.H. Effect of different limitations in chemostat cultures on growth and production of exocellular protease by Bacillus licheniformis. Appl. Microbiol. Biotechnol. 24 (1986) 106-112
25. Daniel W.W. A foundation for analysis in the health sciences: hypothesis testing. In: Daniel W.W. (Ed). Biostatistics. 7th edn. (2000), John Wiley and Sons, Inc., New York 166-167
26. Nam G.W., Lee D.W., Lee H.S., Lee N.J., Kim B.J., and Choe E.A. Native feather degradation by Fervidobacterium islandicum AW-1, a newly isolating keratinase-producing thermophilic anaerobe. Arch. Microbiol. 178 (2002) 538-547
27. Riffel A., Brandelli A., Bellato C.D.M., Souza-Gustavo H.M.F., Eberlin M.N., and Tavares F.C.A. Purification and characterization of a keratinolytic metalloprotease from Chryseobacterium sp. kr6. J. Biotechnol. 128 (2007) 693-703
28. Brandelli A. Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond. Food Bioprocess Technol. 1 (2008) 105-116
29. Riessen S., and Antranikian G. Isolation of Thermoanaerobacter keratinophilius sp. nov, a novel thermophilic, anaerobic bacterium with keratinolytic activity. Extremophile 5 (2001) 399-408
30. Atalo K., and Gashe B.A. Protease production by thermophilic Bacillus sp. (P-001) which degrades various types of fibrous proteins. Biotechnol. Lett. 15 (1993) 1151-1156
31. Ignatova Z., Gousterova A., Spassov G., and Nedkov P. Isolation and partial characterization of extracellular keratinase from a wool degrading thermophilic actinomycete strain Thermoactinomyces candidus. Can. J. Microbiol. 45 (1993) 217-222
32. Varela H., Ferrari M.D., Belobrajdic L., Vazquez A., and Loperena M.L. Skin unhairing proteases of Bacillus subtilis, production and partial characterization. Biotechnol. Lett. 19 (1997) 755-758
33. Maurer K. Detergent proteases. Curr. Opin. Biotechnol. 15 (2004) 330-334
34. Rai S.K., and Mukherjee A.K. Ecological significance and some biotechnological application of an organic solvent stable alkaline serine protease from Bacillus subtilis strain DM-04. Bioresour. Technol. 100 (2009) 2642-2645
35. Farag A.M., and Hasan M.A. Purification, characterization and immobilization of a keratinase from Aspergillus orizae. Enz. Microbiol. Technol. 34 (2004) 85-93
36. Mukherjee A.K. Potential application of cyclic lipopeptide biosurfactants produced by Bacillus subtilis strains in laundry detergents formulations. Lett. Appl. Microbiol. 45 (2007) 330-335