Purification, characterization and immobilization of a keratinase from Aspergillus oryzae

Enzyme and Microbial Technology

Volumn 34, Issue 2, 2004, Pages 85-93

  Farag, Aida M ^a   Hassan, Maha A ^b  

^a NATIONAL INSTITUTE OF OCEANOGRAPHY AND FISHERIES   (Egypt)

^b Alexandria University   (Egypt)

Author keywords

Aspergillus oryzae; Keratinase; Proteolytic activity

Indexed keywords

ACETONE; AMMONIUM COMPOUNDS; CHROMATOGRAPHY; COLLAGEN; ELECTROPHORESIS; ENZYME IMMOBILIZATION; ETHANOL; FILTRATION; MONOMERS; PH EFFECTS; PRECIPITATION (CHEMICAL); PURIFICATION;

ION EXCHANGE CHROMATOGRAPHY;

ENZYMES;

ACETONE; ALCOHOL; AMMONIUM SULFATE; BARIUM ION; BOVINE SERUM ALBUMIN; CALCIUM ION; CASEIN; COLLAGEN; EDETIC ACID; ETHANOLAMINE DERIVATIVE; FUNGAL ENZYME; KERATIN; KERATINASE; LEAD;

ANALYTICAL EQUIPMENT; ASPERGILLUS ORYZAE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME IMMOBILIZATION; ENZYME INHIBITION; ENZYME ISOLATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SUBSTRATE; FEATHER; FRACTIONATION; FUNGUS CULTURE; GEL FILTRATION; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRECIPITATION; PROTEIN DEGRADATION; REVIEW; TEMPERATURE; THERMOSTABILITY; WOOL;

ANAS SP.; ASPERGILLUS ORYZAE; BOVINAE; GALLUS GALLUS; OVIS ARIES;

EID: 0347683371     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2003.09.002     Document Type: Review

References (34)

1. Abdel-Naby M.A. Immobilization of Aspergillus niger NRC 107 xylanase and B-xylosidase and properties of the immobilized enzymes. Appl. Biochem. Biotechnol. 38:1993;69-81.
2. Arica M.Y., Alaeddinoglu N.G., Hasirci V. Immobilization of glucoamylase onto activated pHEMA/EGDMA microspheres: properties and application to a packed-bed reactor. Enz. Microbial. Tech. 22:1998;152-157.
3. Asahi MR, Lindquist K, Fukuyama G, Apodaca WL, Epstein, Mckerrow JH. Purification and charaterization of major extracellular proteinases from Trichophyton rubrum. Biochem J 1985;232:139-44.
4. Atalo K., Gashe B.A. Protease production by a thermophilic Bacillus species (P-001A) which degrades various kinds of fibrous proteins. Biotechnol. Lett. 15:1993;1151-1156.
5. Bergkvist R. The proteolytic enzymes of Aspergillus oryzae. I. Methods for estimation and isolation of the proteolytic enzymes. Acta Chem. Scand. 17:1963;1521-1540.
6. Birch GG, Parler KJ, Worgan JT. Food from waste. In: Enzyme and food processing. London: Applied Science; 1976. p. 19-65.
7. Bockle B., Galinsky B., Muller R. Characterization of a keratinolytic serine proteinase from Streptomyces pactum DSM 40530. Appl. Environ. Microbiol. 61:1995;3705-3710.
8. Chandrasekaran S., Dhar S.C. Utilization of multiple proteinase concentrate to improve the nutritive value of chicken feather meal. J. Leather Res. 4:1986;23-30.
9. Chen S.Y., Hardin C.C., Swaisgood H.E. Purification and characterization of B- structural domains of B-lactoglobulin liberated by immobilized proteolytsis. J. Protein Chem. 12:1993;613-625.
10. Chessen A. Improving the nutritional value of feeds for pigs and poultry with enzyme supplement-current benefits and future prospects. In: Enzymes in der tierenahrung. Zurich: Institut fur Nutzlierwissenchaften; 1990. p. 25-37.
11. Chitte R.R., Nalawade V.K., Dey S. Keratinolytic activity from the broth of a feather-degrading thermophilic streptomyces thermoviolaceus strain SD8. Lett. Appl. Microbiol. 28:1999;131-136.
12. Church F.C., Swaigood H.E., Catignani G.L. Compositional analysis of proteins following hydrolysis by immobilized proteinase. J. Appl. Biochem. 6:1984;205-211.
13. Friedrich A.B., Antranikian G. Keratin degradation by Frevidobacterium pennavorans, a novel thermophilic anaerobic species of the order thermotogales. Appl. Environ. Microbiol. 62:1996;2875-2882.
14. Godfrey T. Protease in waste treatment. In: Godfrey T, editor. Industrial enzymology. London: Macmillan; 1996. p. 315-6.
15. Gradisar H., Kern S., Friedrich J. Keratinase of Doratomyces microsporus. Appl. Microbiol. Biotechnol. 53:2000;196-200.
16. Ignatova Z., Gousterova A., Spassov G., Nedkov P. Isolation and partial characterization of extracellular keratinase from a wool degrading thermophilic actinomycete strain Thermoactinomyces candidus. Can. J. Microbiol. 45:1999;217-222.
17. Ivony K., Szajani B., Seres G. Immobilization of starch-degrading enzymes. I. A comparative study on soluble and immobilized cyclodextrin glucosyltransferase. J. Appl. Biochem. 5:1983;158-164.
18. Janolino V.G., Swaisgood H.E. Analysis and optimization of methods using water-soluble carbodimide for immobilization of biochemicals to porous glass. Biotechnol. Bioeng. 624:1982;1069-1080.
19. Kusana S., Shiraish T., Takahashi S.I., Fujimoto D., Sakano Y. Immobilization of Bacillus acidopullulyticus pullulanase and properties of the immobilized pullulanase. J. Ferment. Bioeng. 68:1989;233-237.
20. Laemmli U.K. Cleavage of structural protein during the assembly of head of bacteriophage T4. Nature (London). 227:1970;680-685.
21. I-02: a new isolated strain. Lett. Appl. Microbiol. 26:1998;77-80.
22. Lin X., Lee C.G., Casale E.S., Shih J.C.H. Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain. Appl. Environ. Microbiol. 58:1992;3271-3275.
23. Lin X., Shih J.C.H., Swaisgood E.H. Hydrolysis of feather keratin by immobilized keratinase. Appl. Environ. Microbiol. 62:1996;4273-4275.
24. Lowry O.H., Rosenberg W.J., Farr A.L., Randell R.J. Quantitation of protein using Folin Ciocalteu reagent. J. Biol. Chem. 193:1951;265-275.
25. Mignon B., Swinnen M., Bouchara J.P., Holinger M., Nikkels A., Pierard G.et al. Purification and characterization of a 315 kDa keratinolytic subtilisin like serine protease from Microsporum canis and evidence of its secretion in naturally infected cats. Med. Mycol. 36:1998;395-404.
26. Mukhopadyay R.P., Chandra A.L. Keratinase of a streptomycete. Ind. J. Exp. Biol. 28:1990;575-577.
27. Ohtakara A., Mitsutomi M. Immobilization of thermostable - α-galactosidase from Pycnoporus cinnabarinus on chitosan beads and its application to the hydrolysis of raffinose in beat sugar molasses. J. Ferment. Technol. 65:1987;493-496.
28. Papadopulos M.C. Effect of processing on high-protein feed stuffs: a review. Biol. Wastes. 29:1989;123-138.
29. Qin L.M., Dekio S., Jiodoi J. Some biochemical characteristics of a partially purified extracellular keratinase from Trichnophyton schoenleinii. Zentralbl Bakteriol. 277(2):1992;236-244.
30. Safranek W.W., Goos R.D. Degradation of wool by saprophytic fungi. Can. J. Microbiol. 28:1982;137-140.
31. Siesenop U., Bohm K.H. Comparative studies on keratinase production of Trichophyton mentagrophytes strains of animal origin. Mycoses. 38:1995;205-209.
32. Swaisgood H.E., Catignani G.L. Use of immobilized proteinases and peptidases to study structural changes in proteins. Meth. Enzymol. 135:1987;596-604.
33. Takichi I., Higuchi D., Sei Y., Koga M. Isolation of an extracellular proteinase (Keratinase) from Microsporium canis. Sabrouraudia. 20:1982;281-288.
34. Young R.A., Smith R.E. Degradation of feather keratin by culture filtrates of Streptomyces fradiae. Can. J. Microbiol. 21:1975;583-586.