Optimalization of preparation of apocytochrome b5 utilizing apo-myoglobin

Interdisciplinary Toxicology

Volumn 1, Issue 2, 2008, Pages 190-192

  Mrázová, Barbora ^a   Martínková, Markéta ^a   Martínek, Václav ^a   Stiborová, Marie ^a   Frei, Eva ^b  

^a CHARLES UNIVERSITY   (Czech Republic)

^b GERMAN CANCER RESEARCH CENTER DKFZ   (Germany)

Author keywords

apo myoglobin; butanon extraction; cytochrome b5; PH

Indexed keywords

EID: 67349084132     PISSN: 13376853     EISSN: 13379569     Source Type: Journal    
DOI: 10.2478/v10102-010-0037-8     Document Type: Article

References (9)

1. Altuve A, Lijun Wang L, Benson DR and Rivera M. (2004) Mammalian mitochondrial and microsomal cytochromes b5 exhibit divergent structural and biophysical characteristics. Biochem Biophys Res Comm 314: 602-609.
2. Hargrove MS, Barrick D and Olson JS (1996) The Association Rate Constant for Heme Binding to Globin Is Independent of Protein Structure. Biochemistry 35: 11293-11299.
3. Hargrove MS, Singleton EW, Quillin ML, Ortiz LA, Phillips GN, Jr., Olson JS and Mathews AJ (1994) His64- (E7)-Tyr apomyoglobin as a reagent for measuring rates of hemin dissociation. J Bio Chem 269: 4207-4214.
4. Miksanova M, Igarashi J, Minami M, Sagami I, Yamauchi S, Kurokawa H and Shimizu T (2006) Characterization of heme-regulated eIF2alpha kinase: roles of the N-terminal domain in the oligomeric state, heme binding, catalysis, and inhibition. Biochemistry 45: 9894-905.
5. Rossi-Fanelli A, Antonini E, Caputo A. (1959) Studies on the structure of hemoglobin. II. Properties of reconstituted protohemoglobin and protoporphyringlobin. Biochim Biophys Acta 35: 93-101.
6. Shenkman JB and Jansson I. (2003) The many roles of cytochrome b5. Pharm Therap 97: 139-152.
7. Stiborová M, Martínek V, Schmeiser HH and Frei E. (2006) Modulation of CYP1A1-mediated oxidation of carcinogenic azo dye Sudan I and its binding to DNA by cytochrome b5. Neuro Endocrinol Let 27 (Suppl. 2): 35-39.
8. Wiechelman KJ, Braun RD, Fitzpatrick JD. (1988) Investigation of the bicinchoninic acid protein assay: identification of the groups responsible for color formation. Anal Biochem 175: 231-237.
9. Yamazaki H, Shimada T, Martin MV and Guengerich FP. (2001) Stimulation of cytochrome P450 reactions by apo-cytochrome b5: evidence against transfer of heme from cytochrome P450 3A4 to apo-cytochrome b5 or heme oxygenase. J Biol Chem 276: 30885-30891.