Quantitative evaluation of six different viral suppressors of silencing using image analysis of transient GFP expression

Plant Cell Reports

Volumn 28, Issue 4, 2009, Pages 639-647

  Dhillon, Taniya ^a   Chiera, Joseph M ^a   Lindbo, John A ^a,b   Finer, John J ^a  

^a OHIO STATE UNIVERSITY   (United States)

^b Campbell's Seeds   (United States)

Author keywords

Gene silencing; GFP; Lima bean cotyledons; Particle bombardment; RNA silencing; Viral gene silencing suppressors

Indexed keywords

BARLEY STRIPE MOSAIC VIRUS; BEET YELLOWS VIRUS; BETA VULGARIS SUBSP. VULGARIS; HORDEUM; LYCOPERSICON ESCULENTUM; NICOTIANA TABACUM; PHASEOLUS LUNATUS; TOBACCO ETCH VIRUS; TOBACCO MOSAIC VIRUS; TOMATO BUSHY STUNT VIRUS; TOMATO GOLDEN MOSAIC VIRUS;

GREEN FLUORESCENT PROTEIN;

ARTICLE; BEAN; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; METABOLISM; PLANT VIRUS; TRANSGENIC PLANT;

GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GREEN FLUORESCENT PROTEINS; PHASEOLUS; PLANT VIRUSES; PLANTS, GENETICALLY MODIFIED;

EID: 67049136599     PISSN: 07217714     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00299-009-0675-5     Document Type: Article

References (56)

1. F Altpeter N Baisakh R Beachy R Bock T Capell P Christou H Daniell K Datta S Datta PJ Dix C Fauquet N Huang A Kohli H Mooibroek L Nicholson TT Nguyen G Nugent K Raemakers A Romano DA Somers E Stoger N Taylor R Visser 2005 Particle bombardment and the genetic enhancement of crops: myths and realities Mol Biol 5 305 327 (Pubitemid 41201795)
2. R Anandalakshmi GJ Pruss X Ge R Marathe AC Mallory TH Smith VB Vance 1998 A viral suppressor of gene silencing in plants Proc Natl Acad Sci USA 95 13079 13084 (Pubitemid 28509527)
3. DC Baulcombe 2004 RNA silencing in plants Nature 431 356 363
4. DM Bisaro 2006 Silencing suppression by geminivirus proteins Virology 344 158 168 (Pubitemid 41814460)
5. MT Buenrostro-Nava PP Ling JJ Finer 2005 Development of an automated image acquisition system for monitoring gene expression and tissue growth Trans Am Soc for Agric Eng 48 841 847 (Pubitemid 40764296)
6. KMJ Butaye BPA Camune SL Delaure MFC De Bolle 2005 Approaches to minimize variation in transgene expression in plants Mol Breed 16 79 91 (Pubitemid 41298035)
7. EJ Chapman AI Prokhnevsky K Gopinath VV Dolja JC Carrington 2004 Viral RNA silencing suppressors inhibit the microRNA pathway at an intermediate step Gene Dev 18 1179 1186 (Pubitemid 38669038)
8. JM Chiera RA Bouchard SL Dorsey E Park MT Buenrostro-Nava PP Ling JJ Finer 2007 Isolation of two highly active soybean (Glycine max (L.) Merr.) promoters and their characterization using a new automated image collection and analysis system Plant Cell Rep 26 1501 1509 (Pubitemid 47282770)
9. JM Chiera JA Lindbo JJ Finer 2008 Quantification and extension of transient GFP expression by the co-introduction of a suppressor of silencing Transgenic Res 17 1143 1154
10. S DeBuck A Jacobs M VanMontagu A Depicker 1998 Agrobacterium tumefaciens transformation and cotransformation frequencies of Arabidopsis thaliana root explants and tobacco protoplasts Mol Plant Microbe Interact 6 449 457 (Pubitemid 28244570)
11. S-W Ding O Voinnet 2007 Antiviral immunity directed by small RNAs Cell 130 413 426 (Pubitemid 47198311)
12. XS Ding J Liu N-H Cheng A Folimonov Y-M Hou Y Bao C Katagi SA Carter RS Nelson 2004 The Tobacco mosaic virus 126-kDa protein associated with virus replication and movement suppresses RNA silencing Mol Plant Microb Interact 17 583 592 (Pubitemid 38642202)
13. P Dunoyer C-H Lecellier EA Parizotto C Himber O Voinnet 2004 Probing the microRNA and small interfering RNA pathways with virus-encoded suppressors of RNA silencing Plant Cell 16 1235 1250 (Pubitemid 38641517)
14. Finer JJ, Dhillon T (2008) Transgenic plant production. In: Stewart CN (ed) Plant biotechnology and genetics: principles, techniques and applications. Wiley, New York, pp 245-272
15. JE Finer JJ Finer 2007 A simple method for reducing moisture condensation on Petri dish lids Plant Cell Tiss Org Cult 91 299 304 (Pubitemid 47632212)
16. JJ Finer P Vain MW Jones MD McMullen 1992 Development of the particle inflow gun for DNA delivery to plant cells Plant Cell Rep 11 323 328
17. Finer JJ, Finer KR, Santarém ER (1996) Plant cell transformation, physical methods for. In: Meyers RA (ed) Encyclopedia of molecular biology and molecular medicine. VCH Publishers, Weinheim, pp 458-465
18. Finer JJ, Finer KR, Ponappa T (1999) Particle bombardment-mediated transformation. In: Hammond J, McGarvey PB, Yusibov V (eds) Current topics in microbiology and immunology, plant biotechnology: new products and applications, vol 240. Springer, Heidelberg, pp 59-80
19. Finer JJ, Beck SL, Buenrostro-Nava MT, Chi YT, Ling PP (2006) Monitoring gene expression in plant tissues; using green fluorescent protein with automated image collection and analysis. In: Dutta Gupta S, Ibaraki Y (eds) Plant tissue culture engineering; focus in biotechnology. Springer, Dordrecht, pp 31-46
20. OL Gamborg RA Miller K Ojima 1968 Nutrient requirements of suspension cultures of soybean root cells Exp Cell Res 50 151 158
21. R Hunold R Bronner G Hahne 1994 Early events in microprojectile bombardment: cell viability and particle location Plant J 5 593 604
22. LK Johansen JC Carrington 2001 Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system Plant Physiol 126 930 938 (Pubitemid 32656780)
23. JDG Jones JL Dangl 2006 The plant immune system Nature 444 323 329 (Pubitemid 44764102)
24. KD Kasschau JS Carrington 1998 A counter-defensive strategy of plant viruses: suppression of posttranscriptional gene silencing Cell 95 461 470 (Pubitemid 28533303)
25. KD Kasschau Z Xie E Allen C Llave EJ Chapman KA Krizan JC Carrington 2003 P1/HCPro, a viral suppressor of RNA silencing, interferes with Arabidopsis development and miRNA function Dev Cell 4 205 217 (Pubitemid 36221797)
26. TM Klein T Gradziel ME Fromm JC Sanford 1988 Factors influencing gene delivery into Zea mays cells by high-velocity microprojectiles BioTechnol 6 559 563
27. K Kubota S Tsuda A Tamai T Meshi 2003 Tomato mosaic virus replication protein suppresses virus-targeted posttranscriptional gene silencing J Virol 77 11016 11026 (Pubitemid 37204293)
28. L Lakatos G Szittya D Silhavy J Burgyán 2004 Molecular mechanism of RNA silencing suppression mediated by p19 protein of tombusviruses EMBO J 23 876 884 (Pubitemid 38418754)
29. L Lakatos T Csorba V Pantaleo EJ Chapman JC Carrington Y-P Liu VV Dolja LF Calvino JJ López-Moya J Burgyán 2006 Small RNA binding is a common strategy to suppress RNA silencing by several viral suppressors EMBO J 25 2768 2780 (Pubitemid 43980384)
30. F Li S-W Ding 2006 Virus counterdefense: diverse strategies for evading the RNA silencing immunity Ann Rev Microbiol 60 503 531 (Pubitemid 44627959)
31. H Li WX Li S-W Ding 2002 Induction and suppression of RNA silencing by an animal virus Science 296 1319 1321 (Pubitemid 34522787)
32. R Lu A Folimonov M Shintaku W-X Li BW Falk WO Dawson S-W Ding 2004 Three distinct suppressors of RNA silencing encoded by a 20-kb viral RNA genome Proc Natl Acad Sci USA 101 15742 15747 (Pubitemid 39473558)
33. AC Mallory L Ely TH Smith R Marathe R Anandalakshmi M Fagard H Vaucheret G Pruss L Bowman VB Vance 2001 HC-Pro suppression of transgene silencing eliminates the small RNAs but not transgene methylation or the mobile signal Plant Cell 13 571 583 (Pubitemid 32265376)
34. T Murashige F Skoog 1962 A revised medium for rapid growth and bioasssays with tobacco tissue culture Physiol Plant 15 473 497
35. WP Pawlowski DA Somers 1998 Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA Proc Natl Acad Sci USA 95 12106 12110 (Pubitemid 28483726)
36. T Ponappa AE Brzozowski JJ Finer 1999 Transient expression and stable transformation of soybean using the jellyfish green fluorescent protein Plant Cell Rep 19 6 12 (Pubitemid 30106660)
37. F Prols P Meyer 1992 The methylation patterns of chromosomal integration regions influence gene activity of transferred DNA in Petunia hybrida Plant J 2 465 475
38. Rasband WS (1997-2008) ImageJ, US National Institutes of Health, Bethesda, Maryland, USA. http://www.rsb.info.nih.gov/ij/
39. JC Reed KD Kasschau AI Prokhnevsky K Gopinath GP Pogue JC Carrington VV Dolja 2003 Suppressor of RNA silencing encoded by Beet yellows virus Virology 306 203 209 (Pubitemid 36299268)
40. HA Richards MD Halfhill RJ Millwood CN Stewart 2003 Quantitative GFP fluorescence as an indicator of recombinant protein synthesis in transgenic plants Plant Cell Rep 22 117 121 (Pubitemid 37224188)
41. BM Roth GJ Pruss VB Vance 2004 Plant viral suppressors of RNA silencing Virus Res 102 97 108 (Pubitemid 38447254)
42. G Roudet-Tavert S German-Retana T Delaunay B Delécolle T Candresse O LeGall 2002 Interaction between potyvirus helper component-proteinase and capsid protein in infected plants J Gen Vir 83 1765 1770 (Pubitemid 34716284)
43. PK Sahoo S Soltani AKC Wong 1988 A survey of thresholding techniques Comput Vision Graph 41 233 260 (Pubitemid 18577086)
44. D Silhavy A Molnár A Lucioli G Szittya C Honryik M Tavazza J Burgyán 2002 A viral protein suppresses RNA silencing and binds silencing-generated, 21- to 25-nucleotide double-stranded RNAs EMBO J 21 3070 3080 (Pubitemid 34670391)
45. SK Svitashev WP Pawlowski I Makarevitch DW Plank DA Somers 2002 Complex transgene locus structures implicate multiple mechanisms for plant transgene rearrangement Plant J 32 433 445 (Pubitemid 36814952)
46. P Thévanz UE Ruttimann M Unser 1998 A pyramid approach to subpixel registration based on intensity IEEE Trans Image Process 7 27 41 (Pubitemid 128745320)
47. O Voinnet 2001 RNA silencing as a plant immune system against viruses Trends Genet 17 449 459 (Pubitemid 32727237)
48. O Voinnet YM Pinto DC Baulcombe 1999 Suppression of gene silencing: a general strategy used by diverse DNA and RNA viruses of plants Proc Natl Acad Sci USA 96 14147 14152
49. H Wang KJ Buckley X Yang RC Buchmann DM Bisaro 2005 Adenosine kinase inhibition and suppression of RNA silencing by Geminivirus AL2 and L2 Proteins J Virol 79 7410 7418 (Pubitemid 40756778)
50. R Weld J Heinemann C Eady 2001 Transient GFP expression in Nicotiana plumbaginifolia suspension cells: the role of gene silencing, cell death, and T-DNA loss Plant Mol Biol 45 377 385 (Pubitemid 32382752)
51. Wu C, Chiera JM, Ling PP, Finer JJ (2008) Isoxaflutole treatment leads to reversible tissue bleaching and allows for more effective detection of GFP in transgenic soybean tissues. In Vitro Cell Develop Biol Plant 6:540-547
52. K Ye DJ Patel 2005 RNA silencing suppressor p21 of Beet yellows virus forms an RNA binding octameric ring structure Structure 13 1375 1384 (Pubitemid 41262104)
53. NE Yelina NI Savenkov AG Solovyev SY Morozov JPT Valkonen 2002 Long-distance movement, virulence and RNA silencing suppression controlled by a single protein in hordei- and potyviruses: complementary functions between virus families J Virol 76 12981 12991 (Pubitemid 35386982)
54. S-D Yoo Y-H Cho J Sheen 2007 Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis Nat Protocols 2 1565 1572 (Pubitemid 47028844)
55. B Yu EJ Chapman Z Yang JC Carrington X Chen 2006 Transgenically expressed viral RNA silencing suppressors interfere with microRNA methylation in Arabidopsis FEBS Lett 580 3117 3120 (Pubitemid 43729657)
56. X Zhou R Carranco S Vitha TC Hall 2005 The dark side of green fluorescent protein New Phytol 168 313 322 (Pubitemid 43919582)