Two site-directed mutations are required for the conversion of a sugar dehydratase into an aminotransferase

Biochemistry

Volumn 48, Issue 23, 2009, Pages 5246-5253

  Cook, Paul D ^a   Kubiak, Rachel L ^a   Toomey, Daniel P ^b   Holden, Hazel M ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b Edgewood Campus Middle School ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE ARCHITECTURE; AMINATION REACTION; AMINOTRANSFERASE; ASPARTATE AMINOTRANSFERASE; BIOSYNTHETIC PATHWAY; COLITOSE; E. COLI; GRAM-NEGATIVE BACTERIA; HYDROXYL GROUPS; MUTANT PROTEINS; SALMONELLA ENTERICA; SITE-DIRECTED MUTATION; SYNTHASE; THREE-DIMENSIONAL STRUCTURE; VIBRIO CHOLERAE;

AMINES; BACTERIA; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOSYNTHESIS; CATALYSTS; ENZYMES; ESCHERICHIA COLI; PLANTS (BOTANY); SUGAR (SUCROSE);

SUGARS;

ASPARTATE AMINOTRANSFERASE; GUANOSINE DIPHOSPHATE 4 KETO 6 DEOXYMANNOSE 3 AMINOTRANSFERASE; GUANOSINE DIPHOSPHATE 4 KETO 6 DEOXYMANNOSE 3 DEHYDRATASE; GUANOSINE DIPHOSPHATE PEROSAMINE SYNTHASE; MUTANT PROTEIN; PYRIDOXAL 5 PHOSPHATE; UNCLASSIFIED DRUG;

ARTICLE; CAULOBACTER CRESCENTUS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; ESCHERICHIA COLI; ISOENZYME ANALYSIS; MUTATION; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION;

BACTERIAL PROTEINS; BINDING SITES; CARBOHYDRATE EPIMERASES; CAULOBACTER CRESCENTUS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI PROTEINS; GUANOSINE DIPHOSPHATE MANNOSE; HYDRO-LYASES; KINETICS; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; SUBSTRATE SPECIFICITY; TRANSAMINASES;

CAULOBACTER VIBRIOIDES; ESCHERICHIA COLI; NEGIBACTERIA; SALMONELLA ENTERICA; VIBRIO CHOLERAE;

EID: 67049119446     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9005545     Document Type: Article

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