A rapid, fluorescence-based assay for detecting antigenic modulation of the acetylcholine receptor on human cell lines

Cytometry Part B - Clinical Cytometry

Volumn 76, Issue 3, 2009, Pages 206-212

  Keefe, Dennis ^a   Hess, Donna ^a   Bosco, Jennifer ^a   Tzartos, Socrates ^b   Powell, Jan ^a   Lamsa, Justin ^a   Josiah, Serene ^a  

^a SHIRE   (United States)

^b HELLENIC PASTEUR INSTITUTE   (Greece)

Author keywords

Acetylcholine receptor; Antigenic modulation; Flow cytometry; Myasthenia gravis

Indexed keywords

CHOLINERGIC RECEPTOR; CHOLINERGIC RECEPTOR ANTIBODY; MONOCLONAL ANTIBODY;

ANTIBODY DETECTION; ANTIBODY LABELING; ANTIBODY SPECIFICITY; ANTIGENIC MODULATION; ARTICLE; CELL LINE; CONTROLLED STUDY; DIAGNOSTIC VALUE; FLOW CYTOMETRY; FLUORESCENCE ANALYSIS; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; MUSCLE CELL; MYASTHENIA GRAVIS; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN DOMAIN; RECEPTOR BINDING; RECEPTOR DOWN REGULATION;

ANTIBODIES, MONOCLONAL; ANTIGEN-ANTIBODY REACTIONS; AUTOANTIBODIES; CELL LINE; DOWN-REGULATION; FLOW CYTOMETRY; FLUORESCENCE; HUMANS; MYASTHENIA GRAVIS; RECEPTORS, CHOLINERGIC; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

EID: 66949146079     PISSN: 15524949     EISSN: 15524957     Source Type: Journal    
DOI: 10.1002/cyto.b.20454     Document Type: Article

References (19)

1. Conti-Fine BM, Milani M, Kaminski HJ. Myasthenia gravis: Past, present, and future. J Clin Invest 2006;116:2843-2854.
2. Heinemann S, Bevan S, Kullberg R, Lindstrom J, Rice J. Modulation of acetylcholine receptor by antibody against the receptor. Proc Natl Acad Sci USA 1977;74:3090-3094.
3. Reiness CG, Weinberg CB, Hall ZW. Antibody to acetylcholine receptor increases degradation of junctional and extrajunctional receptors in adult muscle. Nature 1978;274:68-70.
4. Merlie JP, Heinemann S, Einarson B, Lindstrom JM. Degradation of acetylcholine receptor in diaphragms of rats with experimental autoimmune myasthenia gravis. J Biol Chem 1979;254:6328-6332.
5. Lindstrom J, Einarson B. Antigenic modulation and receptor loss in experimental autoimmune myasthenia gravis. Muscle Nerve 1979;2: 173-179.
6. Psaridi-Linardaki L, Trakas N, Mamalaki A, Tzartos SJ. Specific immunoadsorption of the autoantibodies from myasthenic patients using the extracellular domain of the human muscle acetylcholine receptor alpha-subunit. Development of an antigen-specific therapeutic strategy. J Neuroimmunol 2005;159:183-191.
7. Newsom-Davis J, Pinching AJ, Vincent A, Wilson SG. Function of circulating antibody to acetylcholine receptor in myasthenia gravis: Investigation by plasma exchange. Neurology 1978;28:266-272.
8. Lindstrom JM, Einarson BL, Lennon VA, Seybold ME. Pathological mechanisms in experimental autoimmune myasthenia gravis. I. Immunogenicity of syngeneic muscle acetylcholine receptor and quantitative extraction of receptor and antibody-receptor complexes from muscles of rats with experimental autoimmune myasthenia gravis. J Exp Med 1976;144:726-738.
9. Buschman E, van Oers N, Katz M, Murgita RA. Experimental myasthenia gravis induced in mice by passive transfer of human myasthenic immunoglobulin. Evidence for an ameliorating effect by alpha-fetoprotein. J Neuroimmunol 1987;13:315-330.
10. Tzartos S, Hochschwender S, Vasquez P, Lindstrom J. Passive transfer of experimental autoimmune myasthenia gravis by monoclonal antibodies to the main immunogenic region of the acetylcholine receptor. J Neuroimmunol 1987;15:185-194.
11. Sideris S, Lagoumintzis G, Kordas G, Kostelidou K, Sotiriadis A, Poulas K, Tzartos SJ. Isolation and functional characterization of anti-acetylcholine receptor subunit-specific autoantibodies from myasthenic patients: Receptor loss in cell culture. J Neuroimmunol 2007;189:111-117.
12. Beeson D, Jacobson L, Newsom-Davis J, Vincent A. A transfected human muscle cell line expressing the adult subtype of the human muscle acetylcholine receptor for diagnostic assays in myasthenia gravis. Neurology 1996;47:1552-1555.
13. Loutrari H, Kokla A, Tzartos SJ. Passive transfer of experimental myasthenia gravis via antigenic modulation of acetylcholine receptor. Eur J Immunol 1992;22:2449-2452.
14. Mamalaki A, Trakas N, Tzartos SJ. Bacterial expression of a singlechain Fv fragment which efficiently protects the acetylcholine receptor against antigenic modulation caused by myasthenic antibodies. Eur J Immunol 1993;23:1839-1845.
15. Barchan D, Asher O, Tzartos SJ, Fuchs S, Souroujon MC. Modulation of the anti-acetylcholine receptor response and experimental autoimmune myasthenia gravis by recombinant fragments of the acetylcholine receptor. Eur J Immunol 1998;28:616-624.
16. Psaridi-Linardaki L, Mamalaki A, Remoundos M, Tzartos SJ. Expression of soluble ligand- and antibody-binding extracellular domain of human muscle acetylcholine receptor alpha subunit in yeast Pichia pastoris. Role of glycosylation in alpha-bungarotoxin binding. J Biol Chem 2002;277:26980-26986.
17. Tzartos S, Langeberg L, Hochschwender S, Swanson LW, Lindstrom J. Characteristics of monoclonal antibodies to denatured Torpedo and to native calf acetylcholine receptors: Species, subunit and region specificity. J Neuroimmunol 1986;10:235-253.
18. Barkas T, Gabriel JM, Mauron A, Hughes GJ, Roth B, Alliod C, Tzartos SJ, Ballivet M. Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 6176 of the alpha subunit. J Biol Chem 1988;263:5916-5920.
19. Venkatesh N, Im SH, Balass M, Fuchs S, Katchalski-Katzir E. Prevention of passively transferred experimental autoimmune myasthenia gravis by a phage library-derived cyclic peptide. Proc Natl Acad Sci USA 2000;97:761-766.