Effect of different culture conditions for dextransucrase production in Escherichia coli using lactose as inducer

African Journal of Biotechnology

Volumn 8, Issue 8, 2009, Pages 1577-1582

  Zhang, Hong Bin ^a   Wang, Ya Jie ^a   Hu, Xue Qin ^a   Zhu, Hui Xia ^a   Wei, Zhao Jun ^a  

^a HEFEI UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Components; Culture conditions; Dextransucrase; Lactose; Medium

Indexed keywords

DEXTRANSUCRASE; GLUCOSE; GLYCEROL; LACTOSE; MAGNESIUM SULFATE; SODIUM CHLORIDE;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CULTURE MEDIUM; CULTURE OPTIMIZATION; CULTURE TECHNIQUE; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME SYNTHESIS; ESCHERICHIA COLI; GENETIC ENGINEERING; NONHUMAN; TEMPERATURE SENSITIVITY;

ESCHERICHIA COLI;

EID: 66749158762     PISSN: None     EISSN: 16845315     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Demuth B, Jordening HJ, Buchholz K (1999). Modelling of oligosaccharide synthesis by dextransucrase. Biotechnol. Bioeng. 62: 583-592.
2. Hartwig A (2001). Role of magnesium in genomic stability.Mutat.Res. 45: 113-121.
3. Jeans A, Haynes WC, Williams CA, Rankin JC, Melvin EH, Austin MJ, Cluskey JE, Fisher BE, Tsuchiya HM, Rist CE (1954). Characterization and classification of dextrans from ninety-six strains of bacteria. J.Am.Chem.Soc.76: 5041 -5052.
4. ZP, Zhang X, Tan TW (2006). Lactose-induced production of human soluble B lymphocyte stimulator(hsBLyS)in E.coli with different culture strategies. Biotechnol.Lett.28: 477-483.
5. Malten M, Hollmann R, Deckwer WD, Jahn D (2005). Production and secretion of recombinant Leuconostoc mesenteroides dextransu-crase DsrS in Bacillus megaterium. Biotechnol.bioeng. 89: 207-218.
6. Monchois V, Remaud-Simeon M, Monsan P, Willemot RM (1998). Cloning and sequencing of a gene coding for an extracellur dextransucrase (DSRB) from Leuconostoc mesenteroides NRRL B- 1229 synthesizing only a a(1-6) glucan. FEMS. Microbiol. Lett. 159: 307-315.
7. Monchois V, Remaud-Simeon M, Russell RB, Monsan P, Willemot RM (1997). Characterization of Leuconostoc mesenteroides NRRL B- 512F dextransucrase (DSR-S) and identification of amino-acid residues playing a key role in enzyme activity. Appl. Microbiol. Biotechnol. 48: 465-472.
8. Paliy O, Thusitha S, Gunasekera (2007). Growth of E.coli BL21 in minimal media with different gluconeogenic carbon sources and salt contents. Appl. Microbiol. Bitotechnol. 73: 1169-1172.
9. Richard G, Yu S, Monsan P, Remaud-Simeon M, Morel S (2005). A novel family of glucosyl 1,5-anhydro-D-fructose derivatives synthe- sised by transglucosylation with dextransucrase from Leuconostoc mesenteroides NRRL B-512F. Carbohydrate Res. 340: 395-401.
10. Rodrigues S, Lona LM, Franco T (2003). Effect of phosphate concentration on the production of dextransucrase by Leuconostoc mesenteroides NRRL B512F. Bioprocess Biosyst. Eng. 26: 57-62.
11. Wilson CJ, Zhan H, Swint-Kruse L, Matthews KS (2007). The lactose repressor systems for regulation allosteric behavior and protein folding. Cell. Mol. Life Sci. 64: 3-16.
12. Zhang HB, Hu YJ, Zhu CB, Zhu BQ, Wang YJ (2008a). Cloning, sequencing and expression of a dextransucrase gene (dexYG) from Leuconostoc mesenteroides LM-0326. Biotechnol. Lett. 30: 1441-1446.
13. Zhang HB, Zhu CB, Hu YJ (2008b). Constrution and culture conditions of dextransucrase secreting engineered strain. Acta. Microbiol. Sinica. 48: 492-497.