Characterization of WbpB, WbpE, and WbpD and reconstitution of a pathway for the biosynthesis of UDP-2,3-diacetamido-2,3-dideoxy-D-mannuronic acid in Pseudomonas aeruginosa

Journal of Biological Chemistry

Volumn 284, Issue 18, 2009, Pages 11854-11862

  Westman, Erin L ^a   McNally, David J ^b   Charchoglyan, Armen ^a   Brewer, Dyanne ^a   Field, Robert A ^c   Lam, Joseph S ^a  

^a UNIVERSITY OF GUELPH   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c JOHN INNES CENTRE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYMATIC SYNTHESIS; FUNCTIONAL CHARACTERIZATION; LIPOPOLYSACCHARIDE; MALDI-TOF MASS SPECTROMETRY; MANNURONIC ACIDS; N-ACETYLTRANSFERASE; NMR SPECTROSCOPY; PSEUDOMONAS AERUGINOSA; RECOMBINANT PROTEIN;

ACIDS; BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; CHROMATOGRAPHIC ANALYSIS; ENZYMES; HIGH PRESSURE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; POLYSACCHARIDES; PURIFICATION; SUGAR (SUCROSE); SUGARS; SYNTHESIS (CHEMICAL);

INTERNET PROTOCOLS;

BACTERIAL PROTEIN; HISTAMINE; MANNURONIC ACID; PROTEIN WBPB; PROTEIN WBPD; PROTEIN WBPE; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE 2,3 DIACETAMIDO 2,3 DIDEOXY DEXTRO MANNURONIC ACID; URIDINE DIPHOSPHATE GLUCURONIC ACID; ACYLTRANSFERASE; AMINOTRANSFERASE; HYBRID PROTEIN; LIPOPOLYSACCHARIDE; UDP 2,3 DIACETAMIDO 2,3 DIDEOXY D MANNURONIC ACID; UDP-2,3-DIACETAMIDO-2,3-DIDEOXY-D-MANNURONIC ACID; URONIC ACID;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME MECHANISM; ENZYME PURIFICATION; ENZYME RECONSTITUTION; ENZYME STRUCTURE; ENZYME SUBSTRATE; ENZYME SYNTHESIS; HETEROLOGOUS EXPRESSION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PURIFICATION; PSEUDOMONAS AERUGINOSA; SIGNAL TRANSDUCTION; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETICS; MASS SPECTROMETRY; METABOLISM; METHODOLOGY;

PSEUDOMONAS AERUGINOSA;

ACYLTRANSFERASES; BACTERIAL PROTEINS; LIPOPOLYSACCHARIDES; PSEUDOMONAS AERUGINOSA; RECOMBINANT FUSION PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRANSAMINASES; URONIC ACIDS;

EID: 66449116171     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M808583200     Document Type: Article

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