Isolation, characterisation and kinetic studies on seva TB ES-31 antigen, a metallo-serine protease of interest in serodiagnosis

Indian Journal of Tuberculosis

Volumn 56, Issue 1, 2009, Pages 22-29

  Upadhye, Vijay J ^a   Gomashe, Ashok V ^b   Kumar, Safish ^a   Harinath, Bhaskar C ^a  

^a MAHATMA GANDHI INSTITUTE OF MEDICAL SCIENCES   (India)

^b Shivaji College of Science   (India)

Author keywords

Metallo Serine Protease; Mycobacterium tuberculosis; SEVA TB ES 31 Ag

Indexed keywords

AMMONIUM SULFATE; AZOCASEIN; ENZYME; METALLOSERINE PROTEASE; SERINE PROTEINASE; SEVA TUBERCULOSIS EXCRETORY SECRETORY 31 ANTIGEN; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIGEN PURIFICATION; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CELL SEPARATION; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; FAST PROTEIN LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; LUNG TUBERCULOSIS; MASS SPECTROMETRY; MYCOBACTERIUM TUBERCULOSIS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; SERODIAGNOSIS; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ANTIGENS, BACTERIAL; BIOLOGICAL MARKERS; CELL CULTURE TECHNIQUES; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; HUMANS; MASS SPECTROMETRY; MYCOBACTERIUM TUBERCULOSIS; SERINE ENDOPEPTIDASES; SEROLOGIC TESTS; TUBERCULOSIS;

EID: 66249130680     PISSN: 00195707     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (21)

1. Goldman RC and Laughon BE. Tuberculosis drugs and drug targets. Infectious disorders - drug targets. 2007; 7 (2):71-72.
2. Cole ST et al.Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 1998; 393 :537-544.
3. Nair ER, Banerjee S, Gupta S, Kumar S, Reddy MVR and Harinath BC. Seroreactivity and characterization of 31 kDa M.tb. secretory protease of diagnostic interest in tuberculosis. Trends Clin Biochem Lab Med 2003; 330-338.
4. Shende N, Gupta S, Bhatia AS, Kumar S and Harinath BC. Detection of free and immune-complexed serine protease and its antibody in TB patients with and without HIV coinfection. Int J Tuberc Lung Dis 2005; 9(8): 915-919.
5. 37Rv: a family of subtilicin-like serine proteases. Gene 2000; 254: 147-155.
6. Dave JA, Pittius NCG, Beyers AD, Ehlers MRW and Brown GD. Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages. BMC Microbiol 2002; 2: 1-8.
7. Skeiky YA, Lodes MJ, Guderian JA, Mohamath R, Bement T, Alderson MR and Reed SG. Cloning, expression, and immunological evaluation of two putative secreted serine protease antigens of Mycobacterium tuberculosis. Infect Immun 1999; 67(8): 3998-4007.
8. Cameron RM, Stevenson K, Inglis NF, Klausen J and Sharp JM. Identification and characterization of a putative serine protease expressed in vivo by Mycobacterium avium subsp. paratuberculosis. Microbiology 1994; 140 (Pt 8):1977-82.
9. Pramanik J, Lodam AN, Reddy MVR, Narang P and Harinath BC. Increased yield of excretory secretory antigen with thyroxine supplement in in vitro culture of tubercle bacilli. Indian J Tuberc 1997; 44:185-190.
10. Nair ER, Banerjee S, Kumar S, Reddy MVR. and Harinath BC. Purification and characterization of a 31kDa mycobacterial excretory - secretory antigenic protein with a diagnostic potential in pulmonary tuberculosis. Ind J Chest Dis Allied Sci 2001; 43: 81-90.
11. SSAZOC03-revised 10/14/98, Suitability Assay for Azocasein as a substrate for Trypsin http://www.sigmaaldrich.com/img/assets/18160/ Azocasein_substrate_trypsin.pdf. The date last assessed 16-01-2007
12. O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem 1975; 250: 4007-4021.
13. Harth G, Lee BY, Horwitz MA. High-level heterologous expression and secretion in rapidly growing nonpathogenic mycobacteria of four major Mycobacterium tuberculosis extracellular proteins considered to be leading vaccine candidates and drug targets. Infect Immun 1997; 65(6): 2321-2328.
14. Harth G, Clemens DL and Horwitz MA. Glutamine synthetase of Mycobacterium tuberculosis: extracellular release and characterization of its enzymatic activity. Proc Natl Acad Sci USA 1994; 91:9342-9346.
15. Zhang Y, Lathigra R, Garbe T, Catty D, and Young D. Genetic analysis of superoxide dismutase, the 23 kilodalton antigen of Mycobacterium tuberculosis. Mol Microbiol 1991; 5: 381-391.
16. Belisle JT, Sievert T, Takayama K and Besra GS. Identification of a mycolyltransferase from Mycobacterium tuberculosis: extracellular release and characterization of its enzymatic activity. 1995 p. 212-216. In: Programme of the 30th Joint Conference on Tuberculosis and Leprosy 1995. U.S.-Japan Cooperative Medical Science Program, National Institute of Allergy and Infectious Diseases, National Institute of Health, Ft. Collins, Colo.
17. Markaryan A, Morozova I, Yu H. and Kolattukudy P. Purification and characterization of an elastinolytic metalloprotease of Aspergillus fumigatus and immunoelectron microscopic evidence of this enzyme by the fungus invading the murine lung. Infect Immun 1994; 62(6): 2149-2157.
18. Ishioka GY, Lamont AG, Thomson D, Bulow A, Sette FCA, Grey HM. MHC interaction and T-cell recognition of carbohydrate and glycoproteins. J Immunol 1992; 148:2446-51.
19. Virji M, Saunders Jr, Sims G, Makepeace K, Maskell D, Ferguson DJP. Pilus- facilitated adherence of Neisseria meningitis to human epithelial and endothelial cells: Modulation of adherence phenotype occurs concurrently with changes in primary amino acid sequence and the glycosylation status of pilin. Mol Microbiol 1993; 10: 1013-28.
20. 37Ra bacilli. Scand J Infect Dis 2000; 32: 551-556.
21. Harth G, Lee BY, Horwitz MA. An inhibitor of exported Mycobacterium tuberculosis Glutamine synthetase selectively blocks the growth of pathogenic mycobacteria in axenic culture and in human monocytes:extracellular proteins as potential novel drug targets. J Exp Med 1999; 189(9): 1425-1435.