Creation of a new eye lens crystallin (Gambeta) through structure-guided mutagenic grafting of the surface of βb2 crystallin onto the hydrophobic core of γb crystallin

FEBS Journal

Volumn 276, Issue 12, 2009, Pages 3341-3353

  Kapoor, Divya ^b   Singh, Balvinder ^a,b   Subramanian, Karthikeyan ^b   Guptasarma, Purnananda ^a,b  

^a INSTITUTE OF MICROBIAL TECHNOLOGY   (India)

^b NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Beta sheet remodeling; Lens structural proteins; Protein engineering; Protein folding and stability; Protein surface grafting

Indexed keywords

BETA B 2 CRYSTALLIN; CRYSTALLIN; GAMBETA; GAMMA B CRYSTALLIN; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; CRYOPRECIPITATION; LENS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN STRUCTURE;

AMINO ACID SEQUENCE; BETA-CRYSTALLIN B CHAIN; CALCIUM; CIRCULAR DICHROISM; CRYSTALLINS; ESCHERICHIA COLI; GAMMA-CRYSTALLINS; GENE EXPRESSION; HOT TEMPERATURE; HYDROPHOBICITY; MOLECULAR SEQUENCE DATA; MUTAGENESIS; PROTEIN BINDING; PROTEIN DENATURATION; PROTEIN ENGINEERING; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SOLUBILITY;

EID: 66249117639     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.07059.x     Document Type: Article

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