Cre recombination-mediated cassette exchange for building versatile transgenic human embryonic stem cells lines

Stem Cells

Volumn 27, Issue 5, 2009, Pages 1032-1041

  Du, Zhong Wei ^a   Hu, Bao Yang ^a   Ayala, Melvin ^a   Sauer, Brian ^b   Zhang, Su Chun ^a,c  

^a UNIVERSITY OF WISCONSIN SCHOOL OF MEDICINE AND PUBLIC HEALTH   (United States)

^b STOWERS INSTITUTE FOR MEDICAL RESEARCH   (United States)

^c UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Cre loxP system; Differentiation; Human embryonic stem cells; RMCE; Transgene expression

Indexed keywords

CRE RECOMBINASE;

ARTICLE; CELL DIFFERENTIATION; CELL LINE; ELECTROPORATION; EMBRYONIC STEM CELL; FLOW CYTOMETRY; GENE CASSETTE; GENE EXPRESSION; GENE IDENTIFICATION; GENE LOCUS; GENE SILENCING; GENETIC RESISTANCE; GENETIC TRANSFECTION; GERM LAYER; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; IMMUNOHISTOCHEMISTRY; LOXP SITE; MICROSCOPY; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; STEM CELL EXPANSION; TRANSGENE;

ANIMALS; BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTORS; CELL DIFFERENTIATION; CELL LINE; CELL MEMBRANE PERMEABILITY; EMBRYONIC STEM CELLS; GENE EXPRESSION REGULATION; GENE SILENCING; GREEN FLUORESCENT PROTEINS; HUMANS; INTEGRASES; MICE; MUTAGENESIS, INSERTIONAL; NERVE TISSUE PROTEINS; NEURONS; ORGAN SPECIFICITY; RECOMBINATION, GENETIC; TRANSFECTION; TRANSGENES;

EID: 66149186862     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.38     Document Type: Article

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