Epitope specificity of anti-factor VIII antibodies from inhibitor positive acquired and congenital haemophilia A patients using synthetic peptides spanning A and C domains

Thrombosis and Haemostasis

Volumn 101, Issue 5, 2009, Pages 834-839

  Gharagozlou, Soheila ^a,b   Sharifian, Ramazan A ^c   Khoshnoodi, Jalal ^a   Karimi, Katayoon ^c   Milani, Monica ^d   Okita, David K ^d   Shokri, Fazel ^a,b,e   Conti Fine, Bianca M ^d  

^a TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b PASTEUR INSTITUTE OF IRAN   (Iran)

^c TEHRAN UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^d UNIVERSITY OF MINNESOTA   (United States)

^e AVICENNA RESEARCH INSTITUTE   (Iran)

Author keywords

Epitope mapping; Factor VIII inhibitor; FVIII; Haemophilia A

Indexed keywords

BLOOD CLOTTING FACTOR 8; BLOOD CLOTTING FACTOR 8 INHIBITOR; BLOOD CLOTTING FACTOR 9; PHOSPHATIDYLSERINE; SYNTHETIC PEPTIDE; VON WILLEBRAND FACTOR;

ADULT; AMINO ACID SEQUENCE; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIBODY TITER; ANTIGEN SPECIFICITY; ARTICLE; BINDING SITE; BLOOD SAMPLING; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; FEMALE; HEMOPHILIA A; HUMAN; MALE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN STRUCTURE;

ADULT; ANTIBODY SPECIFICITY; AUTOANTIBODIES; BINDING SITES, ANTIBODY; BINDING, COMPETITIVE; CASE-CONTROL STUDIES; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITOPE MAPPING; EPITOPES; FACTOR VIII; FEMALE; HEMOPHILIA A; HUMANS; IMMUNOGLOBULIN G; MALE; MIDDLE AGED; PEPTIDES; PROTEIN STRUCTURE, TERTIARY;

EID: 66149170090     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/TH08-08-0508     Document Type: Article

References (40)

1. Lenting PJ, Van Mourik JA, Mertens K. The life cycle of coagulation factor VIII in view of its structure and function. Blood 1998; 92: 3983-3996. (Pubitemid 28544310)
2. Hoyer LW. Factor VIII inhibitors. Curr Opin Hematol 1995; 2: 365-371.
3. Pipe SW. Recombinant clotting factors. Thromb Haemost 2008; 99: 840-850.
4. +T Cells to coagulation factor VIII: Response in congenital and acquired haemophilia patients and in healthy subjects. Thromb Haemost 2000; 84: 643-652.
5. Cohen Aj, Kessler CM. Acquired inhibitors. Baillieres Clin Haematol 1996; 9: 331-354. (Pubitemid 26185952)
6. Fulcher CA, Mahoney S, Zimmerman TS. Factor VIII inhibitor IgG subclass and factor VIII polypeptide specificity determined by immunoblotting. Blood 1987; 69: 1475-1480. (Pubitemid 17085310)
7. Scandella D, Mattingly M, Prescott R. A recombinant factor VIII A2 domain polypeptide quantitatively neutralizes human inhibitor antibodies that bind to A2. Blood 1993; 82: 1767-1775. (Pubitemid 23278767)
8. Palmer DS, Dudani AK, Drouin J et al. Identification of novel factor VIII inhibitor epitopes using synthetic peptide arrays. Vox Sang 1997; 72: 148-161. (Pubitemid 27187371)
9. Scandella D, Mattingly M, De Graaf S, et al. Localization of epitopes for human factor VIII inhibitor antibodies by immunoblotting and antibody neutralization. Blood 1989; 74: 1618-1626. (Pubitemid 19251538)
10. Lavigne-Lissalde G, Tarrade C, Lapalud P, et al. Simultaneous detection and epitope mapping of antifactor VIII antibodies. Thromb Haemost 2008; 99: 1090-1096.
11. Healy JF, Lubin IM, Nakai H, et al. Residues 484-508 contain a major determinant of the inhibitors epitope in the A2 domain of human factor VIII. J Biol Chem 1995; 270: 14505-14509.
12. Scandella D, Gilbert GE, Shima M, et al. Some factor VIII inhibitor antibodies recognize a common epitope corresponding to C2 domain amino acids 2248-2312 which overlap a phospholipid binding Site. Blood 1995; 86: 1811-1819.
13. Lollar P. Characterization of factor VIII B-cell inhibitory epitopes. Thromb Haemost 1999; 82: 505-508.
14. Healey JF, Barrow RT, Tamim HM, et al. Residues Glu2181-Val2243 contain a major determinant of the inhibitory epitope in the C2 domain of human factor VIII. Blood 1998; 92: 3701-3709. (Pubitemid 28525197)
15. Kasper C. A more uniform measurement of FVIII inhibitors. Thromb Diath Haemorrh 1975; 34: 869-872.
16. Gharagozlou S, Ghods R, Samadi Bahrami Z, et al. Characterization of human hybridoma clones isolated from haemophilia patients with specificity for different domains of coagulating factor VIII. Hum Antibodies 2003; 12: 67-76. (Pubitemid 37475947)
17. Hu GL, Okita DK, Conti-Fine BM. T cell recognition of the A2 domain of coagulation factor VIII in haemophilia patients and healthy subjects. J Thromb Haemost 2004; 2: 1908-1917. (Pubitemid 40192743)
18. Pratt KP, Qian J, Ellaban E, et al. Immunodominant T-cell epitopes in the factor VIII C2 domain are located within an inhibitory antibody binding site. Thromb Haemost 2004; 92: 522-528. (Pubitemid 39255359)
19. + T cells on the A3 domain of coagulation factor VIII. J Thromb Haemost 2004; 2: 1385-1394.
20. + T-cell epitope repertoire on the C2 domain of coagulation factor VIII. J Thromb Haemost 2003; 1: 1777-1784.
21. + T cells of healthy humans. J Thromb Haemost 2003; 1: 2159-2166.
22. Sugihara T, Takahashi I, Kojima T, et al. Identification of Plasma antibody epitopes and gene abnormalities in Japanese haemophilia A patients with factor VIII inhibitor. Nagoya J Med Sci 2000; 63: 25-39.
23. Giambattista MD, Branckaert T, Laub R. Mapping of natural anti-factor VIII antibodies in plasma pools from healty donors: Use of rationally designed synthetic peptides. Biologicals 2001; 20: 229-232. (Pubitemid 34093645)
24. Albert T, Egler C, Jakuschev S, et al. The B-cell epitope of the monoclonal anti-facto VIII antibody ESH8 characterized by peptide array analysis. Thromb Haemost 2008; 99: 634-637.
25. Scandella D. Epitope specificity and inactivation mechanisms of factor VIII inhibitor antibodies.Vox Sang 1999; 77 (Suppl 1): 17-20.
26. Scandella D. Human anti-factor VIII antibodies: Epitope localization and inhibitory function. Vox Sang 1996; 70 (Suppl 1): 9-14. (Pubitemid 26055988)
27. Scandella DH, Nakai H, Felch M, et al. In haemophilia A and autoantibody inhibitor patients: the factor VIII A2 domain and light chain are most immunogenic. Thromb Res 2001; 101: 377-385.
28. Chaves DG, Velloso-Rodrigues C, Moreau V, et al. Reactivity profile of anti-factor VIII antibodies with designed synthetic peptides mimicking epitopes of the C2 and a1 domains. Br J Haematol 2008; 141: 708-715.
29. Towfighi F, Gharagozlou S, Sharifian RA, et al. Comparative measurement of anti-factor VIII antibody by Bethesda assay and ELISA reveals restricted isotype profile and epitope specificity. Acta Haematol 2005; 114: 84-90. (Pubitemid 41160935)
30. van Helden PM, van den Berg HM, Gouw SC, et al. IgG subclasses of anti-FVIII antibodies during immune tolerance induction in patients with haemophilia A. Br J Haematol 2008; 142: 644-652.
31. Spiegel PC Jr, Stoddard BL. Optimization of factor VIII replacement therapy: Can structural studies help in evading antibody inhibitors? Br J Haematol 2002; 119: 310-322.
32. Van den Brink EN, Turendhout EAM, Bank CMC, et al. Molecular analysis of human anti-factor VIII antibodies by V gene phage display identifies a new epitope in the acidic region following the A2 domain. Blood 2000; 95: 540-545. (Pubitemid 30463373)
33. Saenko El, Scandella D. The acidic region of the factor VIII light chain and the C2 domain together form the high affinity binding site for von Willebrand factor. J Biol Chem 1997; 272: 18007-18014. (Pubitemid 27306379)
34. Gilbert GE, Drinkwater D. Specific membrane binding of factor VIII is mediated by O- Phospho- L-Serine, a moiety of phosphatidylserine. Biochemistry 1993; 32: 9577-9585. (Pubitemid 23296801)
35. Jacquemin MG, Desqueper BG, Benhida A, et al. Mechanism and kinetics of factor VIII inactivation: Study with an IgG4 monoclonal antibody derived from a haemophilia A Patient with inhibitor. Blood 1998; 92: 496-506. (Pubitemid 28323712)
36. Meeks SL, Healey JF, Parker ET, et al. Non-classical anti-C2 domain antibodies are present in patients with factor VIII inhibitors. Blood 2008; 112: 1151-1153.
37. Fulcher CA, Lechner K, Mahoney SDG. Immunoblot analysis shows changes in factor VIII inhibitor chain specificity in factor VIII inhibitor over time. Blood 1988; 72: 1348-1356. (Pubitemid 18265903)
38. Hoyer LW, Scandella D. Factor VIII inhibitors: Structure and function in autoantibody and haemophilia A Patients. Semin Hematol 1994; 31 (Suppl 4): 1-5. (Pubitemid 24185238)
39. Gharagozlou S, Kardar GA, Rabbani H, et al. Molecular analysis of the heavy chain variable region genes of human hybridoma clones specific for coagulation factor VIII. Thromb Haemost 2005; 94: 1131-1137. (Pubitemid 41819606)
40. Bellone M, Karachunski P, Ostlie N, et al. Clustering of B and T epitopes within short sequence regions of the nicotinic acetylcholine receptor. Scand J Immunol 1995; 41: 135-140.