Absolute quantification of phosphorylation on the kinase activation loop of cellular focal adhesion kinase by stable isotope dilution liquid chromatography/mass spectrometry

Analytical Chemistry

Volumn 81, Issue 9, 2009, Pages 3304-3313

  Ciccimaro, Eugene ^a   Hanks, Steven K ^b   Yu, Kenneth H ^c   Blair, Ian A ^a  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c 854 BRB II/III ^*

Author keywords

[No Author keywords available]

Indexed keywords

ABSOLUTE QUANTIFICATION; ACTIVATION LOOPS; ANTICANCER DRUG; CELLULAR SIGNAL TRANSDUCTION; FOCAL ADHESION KINASE; FOCAL ADHESIONS; IMMUNOPRECIPITATION; IN-CELL; IN-VITRO; ISOTOPICALLY LABELED; KINASE ACTIVATION; LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY; MASS BALANCE; MOUSE EMBRYONIC FIBROBLASTS; POST-TRANSLATIONAL MODIFICATIONS; SIGNALING PATHWAYS; STABLE ISOTOPE DILUTION; STABLE ISOTOPES; TRYPSIN DIGESTION; TRYPTIC PEPTIDES; TYROSINE KINASE;

ADHESION; AMINATION; AMINES; AMINO ACIDS; CELL CULTURE; CHEMICAL ACTIVATION; CHROMATOGRAPHIC ANALYSIS; ISOTOPES; LIQUID CHROMATOGRAPHY; LIQUIDS; ORGANIC ACIDS; SIGNAL TRANSDUCTION; SPECTROMETERS; SPECTROMETRY;

PHOSPHORYLATION;

FOCAL ADHESION KINASE; PHOSPHOTRANSFERASE; PROTEIN TYROSINE KINASE; STAT1 PROTEIN; TRYPSIN; YTTRIUM;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ASSAY; ENZYME BINDING; ENZYME PHOSPHORYLATION; FIBROBLAST; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; ISOTOPE DILUTION ASSAY; ISOTOPE LABELING; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; ENZYME ACTIVATION; FOCAL ADHESION PROTEIN-TYROSINE KINASES; HUMANS; IMMUNOPRECIPITATION; ISOTOPE LABELING; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDES; PHOSPHORYLATION; REPRODUCIBILITY OF RESULTS; TRYPSIN;

EID: 66149118232     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac900204f     Document Type: Article

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