Development and evaluation of a multiplex test for the detection of atypical bacterial DNA in community-acquired pneumonia during childhood

Clinical Microbiology and Infection

Volumn 15, Issue 5, 2009, Pages 473-480

  Carrillo, J A ^a   Gutiérrez, José ^b   Garcia F ^c   Munoz A ^b   Villegas, E ^b   Rojas, J ^a   Sorlozano A ^b   Rojas, A ^a  

^a VIRCELL S L   (Spain)

^b UNIVERSITY OF GRANADA   (Spain)

^c HOSPITAL UNIVERSITARIO SAN CECILIO   (Spain)

Author keywords

Chlamydophila pneumoniae; Coxiella burnetii; Legionella spp.; Mycoplasma pneumoniae; Pneumonia

Indexed keywords

BACTERIAL ANTIGEN; BACTERIAL DNA; FUNGAL DNA;

ADOLESCENT; ANALYTIC METHOD; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CHLAMYDOPHILA PNEUMONIAE; COMMUNITY ACQUIRED PNEUMONIA; CONTROLLED STUDY; COXIELLA BURNETII; DNA DETERMINATION; FEMALE; HUMAN; INFANT; LEGIONELLA; MAJOR CLINICAL STUDY; MALE; MULTIPLEX POLYMERASE CHAIN REACTION; MYCOPLASMA PNEUMONIAE; NONHUMAN; PRIORITY JOURNAL; Q FEVER; SENSITIVITY AND SPECIFICITY; SEROLOGY; STREPTOCOCCUS PNEUMONIA; STREPTOCOCCUS PNEUMONIAE;

BACTERIA (MICROORGANISMS); CHLAMYDOPHILA PNEUMONIAE; COXIELLA BURNETII; LEGIONELLA; MYCOPLASMA PNEUMONIAE; STREPTOCOCCUS PNEUMONIAE;

EID: 65949112947     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2009.02711.x     Document Type: Article

References (33)

1. Fernández R, Suárez I, Rubinos G, Medina A, Gullón JA, González I. Neumonía adquirida en la comunidad por gérmenes atípicos: Tratamiento y evolución. Arch Bronconeumol 2006; 42: 430-433.
2. Saldias PF, Pérez CC. National consensus for management of community acquired pneumonia in adults. Rev Chilena Infectol 2005; 22 (suppl 1): 7-10.
3. Khanna M, Fan J, Pehler-Harrington K et al. The pneumoplex assay, a multiplex PCR-enzyme hybridization assay that allows simultaneous detection of five organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei and Bordetella pertussis, and its real-time counterpart. J Clin Microbiol 2005; 43: 565-571.
4. Messmer TO, Skelton SK, Moroney JF, Daugharty H, Fields BS. Application of a nested, multiplex PCR to psittacosis outbreaks. J Clin Microbiol 1997; 35: 2043-2046.
5. Dowell SF, Peeling RW, Boman J et al. Standardizing Chlamydia pneumoniae assays: Recommendations from the centers for disease control and prevention (USA) and the laboratory centre for disease control (Canada). Clin Infect Dis 2001; 33: 492-503.
6. Jonas D, Bosenbaum A, Weyrich S, Bhakdi S. Enzyme-linked immunoassay for detection of PCR-amplified DNA of legionellae in bronchoalveolar fluid. J Clin Microbiol 1995; 33: 1247-1252.
7. Uldum SA, Jensen JS, Sondergard-Andersen J, Lind K. Enzyme immunoassay for detection of immunoglobulin M (IgM) and IgG antibodies to Mycoplasma pneumoniae. J Clin Microbiol 1992; 30: 1198-1204.
8. Munder RU. Other Legionella species. In: Mdell GL, Bennett JE, Dolin R, eds. Principles and practice of infectious diseases, 5th edn, Vol. 2. Philadelphia, PA: Churchill Livingstone, 2000; 2435-2441.
9. Hayden RT, Uhl JR, Qian X et al. Direct detection of Legionella species from bronchoalveolar lavage and open lung biopsy specimens: Comparison of Lightcycler PCR, in.situ hybridization, direct fluorescence, antigen detection and culture. J Clin Microbiol 2001; 39: 2618-2626.
10. Fields BS, Benson RF, Besser RE. Legionella and Legionnaires' disease: 25 years of investigation. Clin Microbiol Rev 2002; 15: 506-526.
11. Waterer GW, Baselski VS, Wunderink RG. Legionella and community-acquired pneumonia: A review of current diagnostic tests from a clinician's viewpoint. Am J Med 2001; 110: 41-48.
12. Rodríguez JC, MasiáM, Gutiérrez F, Royo G. Legionella pneumophila en la neumonía adquirida en la comunidad: interpretación de las pruebas microbiológicas. Med Clin (Barc) 2004; 112: 277-278.
13. Dominguez J, Gali N, Matas L et al. Evaluation of a rapid immunochromatographic assay for the detection of Legionella antigen in urine samples. Eur J Clin Microbiol Infect Dis 1999; 18: 896-898.
14. Raoult F, Urvolgyi J, Etienne J, Roturier M, Puel J, Chaudet H. Diagnosis of endocarditis in acute Q-fever by immunofluorescence serology. Acta Virol 1988; 32: 70-74.
15. Dupuis G, Péter O, Peacock M, Burgdorfer W, Haller E. Immunoglobulin responses in acute Q.fever. J Clin Microbiol 1985; 22: 484-487.
16. Diederen BM, de Jong CM, Marmouk F, Kluytmans JA, Peeters MF, Van der Zee A. Evaluation of real-time PCR for the early detection of Legionella pneumophila DNA in serum samples. J Med Microbiol 2007; 1: 94-101.
17. Gouriet F, Drancourt M, Raoult D. Multiplexed serology in atypical bacterial pneumonia. Ann NY Acad Sci 2006; 1078: 530-540.
18. Stralin K, Korsgaard J, Olcen P. Evaluation of a multiplex PCR for bacterial pathogens applied to bronchoalveolar lavage. Eur Respir J 2006; 28: 568-575.
19. Del Castillo Martin F, García Miguel MJ, García S. Manejo racional de la neumonía aguda de la comunidad. An Esp Pediatr 1999; 51: 609-616.
20. Brines Solanes J, Hernández Marco R. Neumonías agudas en la infancia. In: Cruz Hernández M, ed. Tratado de pediatría, 9th edn, Vol. 2. Madrid: Ergón, 2006; 1323-1339.
21. Liu H, Li Y, Huang W, Kawamura Y, Ezaki T. Use of the dnaj gene for the detection and identification of all Legionella pneumophila serogroups and description of the primers used to detect 16S rDNA gene sequences of major members of the genus Legionella. Microbiol Immunol 2003; 47: 859-869.
22. Zhang GQ, Nguyen SV, To H et al. Clinical evaluation of a new PCR assay for detection of Coxiella burnetii in human serum samples. J Clin Microbiol 1998; 36: 77-80.
23. Stralin K, Backman A, Holmberg H, Fredlund H, Olcen P. Design of a multiplex PCR for Streptococcus pneumoniae, Haemophilus influenzae, Mycoplasma pneumoniae and Chlamydophila pneumoniae to be used on sputum samples. APMIS 2005; 113: 99-111.
24. Ginevra C, Barranger C, Ros A et al. Development and evaluation of Chlamylege, a new commercial test allowing simultaneous detection and identification of Legionella, Chlamydophila pneumoniae, and Mycoplasma pneumoniae in clinical respiratory specimens by multiplex PCR. J Clin Microb 2005; 43: 3247-3254.
25. Meijer TO, Kwakkel GJ, de Vries A, Schouls LM, Ossewaarde JM. Species identification of Chlamydia isolates by analyzing restriction fragment length polymorphism of the 16S-23S rRNA spacer region. J Clin Microbiol 1997; 35: 1179-1183.
26. Madico G, Quinn TC, Boman J, Gaydos CA. Touchdown enzyme time release-PCR for detection and identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci using the 16S and 16S-23S spacer rRNA genes. J Clin Microbiol 2000; 38: 1085-1093.
27. Templeton KE, Scheltinga SA, Graffelman AW et al. Comparison and evaluation of real-time PCR, real-time nucleic acid sequence-based amplification, conventional PCR, and serology for diagnosis of Mycoplasma pneumoniae. J Clin Microbiol 2003; 41: 4366-4371.
28. Corsaro D, Valassina M, Venditti D, Venard V, Le Faou A, Valensin PE. Multiplex PCR for rapid and differential diagnosis of Mycoplasma pneumoniae and Chlamydia pneumoniae in respiratory infections. Diagn Microbiol Infect Dis 1999; 35: 105-108.
29. Tong CYW, Donnelly C, Harvey G, Sillis M. Multiplex polymerase chain reaction for the simultaneous detection of Mycoplasma pneumoniae, Chlamydia pneumoniae, and Chamydia psittaci in respiratory samples. J Clin Pathol 1999; 52: 257-263.
30. Pinar A, Bozdemir N, Kocagoz T, Alacam R. Rapid detection of bacterial atypical pneumonia agents by multiplex PCR. Cent Eur J Public Health 2004; 12: 3-5.
31. Fournier PE, Raoult D. Comparison of PCR and serology assays for early diagnosis of acute Q.fever. J Clin Microb 2003; 41: 5094-5098.
32. Fenollar F, Fournier PE, Raoult D. Molecular detection of Coxiella burnetii in the sera of patients with Q fever endocarditis or vascular infection. J Clin Microbiol 2004; 42: 4919-4924.
33. Petitjean-Lecherbonnier J, Vabret A, Gouarin S, Dina J, Legrand L, Freymuth F. Mycoplasma pneumoniae infections: Retrospective study in Basse-Normandie, 1997-2005. Epidemiology-diagnostic utility of serology and PCR for a rapid diagnostic. Pathol Biol 2006; 54: 603-611.