Touchdown enzyme time release-PCR for detection and identification of Chlamydia trachomatis, C. pneumoniae, and C. psittaci using the 16S and 16S- 23S spacer rRNA genes

Journal of Clinical Microbiology

Volumn 38, Issue 3, 2000, Pages 1085-1093

  Madico, Guillermo ^a,b   Quinn, Thomas C ^a,b   Boman, Jens ^c   Gaydos, Charlotte A ^a  

^a JOHNS HOPKINS UNIVERSITY   (United States)

^b NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES   (United States)

^c UMEÅ UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; RNA 16S; RNA 23S;

ARTICLE; BACTERIUM DETECTION; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CHLAMYDOPHILA PNEUMONIAE; CHLAMYDOPHILA PSITTACI; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; ORNITHOSIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESPIRATORY TRACT INFECTION; VAGINITIS;

ANIMALIA; BACTERIA (MICROORGANISMS); CHLAMYDIA PECORUM; CHLAMYDIA PNEUMONIAE; CHLAMYDIA PSITTACI; CHLAMYDIA TRACHOMATIS; CHLAMYDIAE; CHLAMYDOPHILA PECORUM; CHLAMYDOPHILA PNEUMONIAE; CHLAMYDOPHILA PSITTACI; RICKETTSIALES;

EID: 0034100293     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.38.3.1085-1093.2000     Document Type: Article

References (43)

1. Bauwens, J. G., A. M. Clark, M. J. Loeffelholtz, S. A. Herman, and W. E. Stamm. 1993. Diagnosis of Chlamydia trachomatis urethritis in men by polymerase chain reaction assay of first-catch urine. J. Clin. Microbiol. 31:3013-3016.
2. Black, C. M., P. I. Fields, T. O. Messmer, and B. P. Berdal. 1994. Detection of Chlamydia pneumoniae in clinical specimens by polymerase chain reaction using nested primers. Eur. J. Clin. Microbiol. Infect. Dis. 13:752-756.
3. Bobo, L., F. Coutlee, R. H. Yolken, T. C. Quinn, and R. Viscidi. 1990. Diagnosis of Chlamydia trachomatis cervical infection by detection of amplified DNA with enzyme immunoassay. J. Clin. Microbiol. 28:1968-1973.
4. Bobo, L., B. Munos, R. Viscidi, T. C. Quinn, H. Mkocha, and S. West. 1991. Diagnosis of Chlamydia trachomatis eye infection in Tanzania by polymerase chain reaction/enzyme immunoassay. Lancet 338:847-850.
5. Boman, J., A. Allard, K. Persson, M. Lundborg, P. Juto, and G. Wadell. 1997. Rapid diagnosis of respiratory Chlamydia pneumoniae infection by nested touchdown polymerase chain reaction compared with culture and antigen detection by EIA. J. Infect. Dis. 175:1523-1526.
6. Campbell, L. A., M. P. Melgosa, D. J. Hamilton, C. C. Kuo, and J. T. Grayston. 1992. Detection of Chlamydia pneumoniae by polymerase chain reaction. J. Clin. Microbiol. 30:434-439.
7. Chamberlain, J. S., R. A. Gibbs, J. E. Ranier, P. N. Nguyen, and C. T. Caskey. 1988. Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification. Nucleic Acids Res. 16:11141-11156.
8. Chernesky, M., S. Castriciano, J. Sellor, I. Stewart, I. Cunningham, S. Landis, W. Seidelman, L. Grant, C. Devlin, and J. Mahony. 1990. Detection of Chlamydia trachomatis antigens in urine as an alternative to swabs and cultures. J. Infect. Dis. 161:124-126.
9. Crotchfelt, K. A., B. Pare, C. A. Gaydos, and T. C. Quinn. 1998. Detection of Chlamydia trachomatis by Gen-Probe AMPLIFIED Chlamydia trachomatis Assay (AMP CT) in urine specimens from men and women and endocervical specimens from women. J. Clin. Microbiol. 36:391-394.
10. Don, R. H., P. T. Cox, B. J. Wainwright, K. Baker, and J. S. Mattick. 1991. "Touchdown" PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res. 19:4008.
11. Everett, K. D. E., and A. A. Andersen. 1997. The ribosomal intergenic spacer and domain I of the 23S rRNA gene are phylogenetic markers for Chlamydia spp. Int. J. Syst. Bacteriol. 47:461-473.
12. Feinstein, A. 1985. Clinical epidemiology. The W. B. Saunders Co., Philadelphia, Pa.
13. Fleiss, J. L. 1981. Statistical methods for rates and proportions. 2nd ed. John Wiley & Sons, Inc., New York, N.Y.
14. Gaydos, C. A., T. C. Quinn, and J.J. Eiden. 1992. Identification of Chlamydia pneumoniae by DNA amplification of the 16S rRNA gene. J. Clin. Microbiol. 30:796-800.
15. Gaydos, C. A., L. Palmer, T. C. Quinn, S. Falkow, and J. Eiden. 1993. Phylogenetic relationship of Chlamydia pneumoniae to Chlamydia psittaci and Chlamydia trachomatis as determined by analysis of 16S ribosomal DNA sequences. Int. J. Syst. Bacteriol. 43:610-612.
16. Gaydos, C. A., J. T. Summersgill, N. N. Sahney, J. A. Ramirez, and T. C. Quinn. 1996. Replication of Chlamydia pneumoniae in vitro in human macrophages, endothelial cells, and aortic artery smooth muscle cells. Infect. Immun. 64:1614-1620.
17. Gaydos, C. A., C. L. Fowler, V. J. Gill, J. J. Eiden, and T. C. Quinn. 1993. Detection of Chlamydia pneumaniae by polymerase chain reaction-enzyme immunoassay in an immunocompromised population. Clin. Infect. Dis. 17: 718-723.
18. Gelfand, D. H., and T. J. White. 1990. Thermostable DNA polymerases, p. 129-141. In M. A. Innis, D. H. Gelfand, J. J. Sninsky, and T. J. White (ed.), PCR protocols, a guide to methods and applications. Academic Press, Inc., San Diego, Calif.
19. Hammerschlg, M. R. 1994. Chlamydia trachomatis in children. Pediatr. Ann. 23:349-353.
20. Holland, S. M., C. A. Gaydos, and T. C. Quinn. 1990. Detection and differentiation of Chlamydia trachomatis, Chlamydia psittaci, and Chlamydia pneumoniae hy DNA amplification. J. Infect. Dis. 162:984-987.
21. Jantos, C. A., R. Roggendorf, F. N. Wuppermann, and J. H. Hegemann. 1998. Rapid detection of Chlamydia pneumoniae by PCR-enzyme immunoassay. J. Clin. Microbiol. 36:1890-1894.
22. Jaschek, G., C. A. Gaydos, L. E. Welsh, and T. C. Quinn. 1993. Direct detection of Chlamydia trachomatis in urine specimens from symptomatic men using a rapid polymerase chain reaction assay. J. Clin. Microbiol. 31: 1209-1212.
23. Kay, I. D., S. Palladino, R. Alexander, B. J. Leahy, and J. W. Pearman. 1997. Evaluation of commercial polymerase chain reaction assay for the detection of Chlamydia trachomatis. Diagn. Microbiol. Infect. Dis. 27:75-79.
24. Kuo, C. C., L. A. Jackson, and J. T. Grayston. 1995. Chlamydia pneumoniae (TWAR). Clin. Microbiol. Rev. 8:451-461.
25. Lee, H., M. Chernesky, J. Schachter, J. Burczak, W. Andrews, S. Muldoon, G. Leckie, and W. Stamm. 1995. Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine. Lancet 345:213-216.
26. Logo, M. C., M. S. Beringer, and J. L. Hartley. 1990. Use of uracil DNA glycosylase to control carry-over contamination in polymerase chain reactions. Gene 93:125-128.
27. Maass, M., and K. Dalhoff. 1994. Comparison of sample preparation methods for detection of Chlamydia pneumoniae in bronchioalveolar lavage fluid by PCR. J. Clin. Microbiol. 32:2616-2619.
28. Maass, M., and K. Dalhoff. 1995. Transport and storage conditions for cultural recovery of Chlamydia pneumoniae. J. Clin. Microbiol. 33:1793-1796.
29. Madico, G., T. C. Quinn, A. Rompalo, K. T. McKee, Jr., and C. Gaydos. 1998. Diagnosis of Trichomonas vaginalis infection by PCR using vaginal swab samples. J. Clin. Microbiol. 36:3205-3210.
30. Madico, G., R. H. Gilman, A. Jabra, L. Rojas, H. Hernandez, J. Fukuda, C. Bern, and M. Steinhoff. 1995. The role of pulse oximetry. Its use as an indicator of severe respiratory disease in Peruvian children living at sea level. Arch. Pediatr. Adolesc. Med. 149:1259-1263.
31. Meijer, A., G. J. Kwakkel, A. de Vries, L. M. Schouls, and J. M. Ossewaarde. 1997. Species identification of chlamydia isolates by analyzing restriction fragment length polymorphism of the 16S-23S rRNA spacer region. J. Clin. Microbiol. 35:1179-1183.
32. Messmer, T. O., S. K. Skelton, J. F. Moroney, H. Daugharty, and B. S. Fields. 1997. Application of a nested, multiplex PCR to psittacosis outbreaks. J. Clin. Microbiol. 35:2043-2046.
33. Pudjiatmoko, H. Fukushi, Y. Ochiai, T. Yamaguchi, and K. Hirai. 1997. Phylogenetic analysis of the genus Chlamydia based on 16S rRNA gene sequences. Int. J. Syst. Bacteriol. 47:425-431.
34. Quinn, T. C. 1994. Recent advances in the diagnosis of sexually transmitted diseases. Sex. Transm. Dis. 21(Suppl. 2):S19-S27.
35. Quinn, T. C., L. Welsh, A. Lentz, K. Crotchfelt, J. Zenilman, J. Newhall, and C. A. Gaydos. 1996. Diagnosis by AMPLICOR PCR of Chlamydia trachomatis infection in urine samples from women and men attending sexually transmitted disease clinics. J. Clin. Microbiol. 34:1401-1406.
36. Ramirez, J. A., and the Chlamydia pneumoniae/Atherosclerosis Study Group. 1996. Isolation of Chlamydia pneumoniae from the coronary artery of a patient with coronary atherosclerosis. Ann. Intern. Med. 125:979-982.
37. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harhor, N.Y.
38. Schlossberg, D. 1995. Chlamydia psittaci (psittacosis), p. 1693-1696. In G. L. Mandell, J. E. Bennett, and R. Dolin (ed.), Principles and practice of infectious diseases. Churchill Livingstone, New York, N.Y.
39. Sillis, M., P. White, E. Q. Caul, I. D. Paul, and J. D. Treharne. 1992. The differentiation of Chlamydia species hy antigen detection in sputum specimens from patients with community-acquired acute respiratory infections. J. Infect. 25:77-86.
40. Tong, C. Y. W., and M. Sillis. 1993. Detection of Chlamydia pneumoniae and Chlamydia psittaci in sputum samples by PCR. J. Clin. Pathol. 46:313-317.
41. Toye, B., R. W. Peeling, P. Jessamine, P. Claman, and I. Gemmill. 1996. Diagnosis of Chlamydia trachomatis infections in asymptomatic men and women hy PCR assay. J. Clin. Microbiol. 34:1396-1400.
42. Walsh, P. S., D. A. Metzger, and R. Higuchi. 1991. Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechmqucs 10:506-513.
43. Wilson, P. A., J. Phipps, D. Samuel, and N. A. Saunders. 1996. Development of a simplified polymerase chain reaction-enzyme immunoassay for the detection of Chlamydia pneumoniae. J. Appl. Bacteriol. 80:431-438.